Plgel 5 μm mixed c

The molecular weight was measured employing GPC and depending on the samples and their solubility three different instruments were used.
A GPC instrument coupled with multi-angle light scattering employing an Agilent HPLC was fitted with one guard column and three gel permeation columns (PLgel 5 μm MIXED-C). The differential refractometer (TrEX) and light scattering detector (miniDAWN TREOS) employed are from Wyatt Technology. THF was used as eluent at a flow rate of 1.0 mL min⁻¹. This equipment was employed for PT6HP-co-PγBL copolymers.
A Waters University 1500 GPC with one guard column and two columns (PLgel 5 μm MIXED-C) was used for the measurements carried out at 40 °C and a flow rate of 1.0 mL min⁻¹ using DMF as eluent. This equipment was employed to measure PγBL homopolymer.
A GPC instrument consisting of an Agilent HPLC system with one guard column and two columns (PLgel 5 μm MIXED-C) was coupled with a Wyatt DAWN HELEOS II multi (18)-angle light scattering detector and a Wyatt Optilab Trex dRI detector. The measurements were performed at 40 °C employing chloroform as eluent. PT6HP was measured employing this equipment.

PLA granulate was supplied from Fillamentum Manufacturing Czech s.r.o., Hulín, Czech Republic. Measured polymer parameters were as follows: number-average molecular weight (M_n), 123,500 g/mol; weight-average molecular weight (MW), 235,300 g/mol; dispersity, 1.90. PHB powder was acquired from NAFIGATE Corporation a.s., Prague, Czech Republic. Measured polymer parameters were as follows: number-average molecular weight (M_n), 85,040 g/mol; weight-average molecular weight (MW), 211,400 g/mol; dispersity, 2.49. All measurements of polymer properties were measured via GPC (Agilent 1100, Santa Clara, CA, USA) in chloroform (CHCl₃) and the analysis parameters were as follows: mobile phase flow 1 mL/min; column temperature 30 °C, used column: PLgel 5 μm MIXED-C (300 × 7.5 mm). Aliphatic alcohols for depolymerization (methanol 99%, ethanol 99%) were supplied by Honeywell Research Chemicals, Charlotte, NC, USA (used alcohols were not claimed either synthetic or bio-source by the supplier). The catalyst for alcoholyses (p-toluensulphonic acid monohydrate), methacrylic anhydride (94%), potassium hydroxide (p.a.), d-chloroform (CDCl₃; 99.8%), and 2-ethylhexanoic acid (for synthesis) were all acquired from Sigma-Aldrich, Prague, Czech Republic.