Lc 2010cht chromatography system

All reagents and solvents were used as received. Reactions were monitored by thin layer chromatography on Merck silica gel 60-F₂₅₄-coated 0.25 mm plates, detected by UV. Flash chromatography was performed with the indicated solvents on silica gel (particle size 0.064–0.210 mm). Yields reported are for isolated, spectroscopically pure compounds. HPLC was performed on a SHIMADZU LC-2010CHT chromatography system (Kyoto, Japan) and a CLASS-VPTM analysis data system (Kyoto, Japan). ¹H-NMR spectra were recorded on Brüker AVANCE III-400 spectrometer (Biospin, Switzerland).

All reagents and solvents were used as received. Reactions were monitored by thin-layer chromatography on Merck silica gel 60-F₂₅₄ coated 0.25-mm plates, detected by ultraviolet (UV). Flash chromatography was performed with the indicated solvents on silica gel (particle size 0.064–0.210 mm). Yields reported are for isolated, spectroscopically pure compounds. HPLC was performed on a SHIMADZU LC-2010CHT chromatography system and a CLASS-VPTM analysis data system. ¹HNMR spectra were recorded on a Brüker AVANCE III-400 spectrometer. Chemical shifts are given in ppm (d), referenced to tetramethylsilane (TMS). The letters s, d, t, q, m, and br stand for singlet, doublet, triplet, quartet, multiplet, and broad, respectively. Melting points were recorded on a Mettler Toledo MP70 Melting Point System and are not corrected.