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Recombinant human ccn3

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Recombinant human CCN3 is a protein produced through recombinant DNA technology. It functions as an extracellular matrix-associated protein that plays a role in cellular processes.

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Lab products found in correlation

Anti mouse and anti rabbit igg conjugated horseradish peroxidase, by Santa Cruz Biotechnology (1 mentions) Sc 136967, by Santa Cruz Biotechnology (1 mentions) β actin sc 130656, by Santa Cruz Biotechnology (1 mentions) Protein a g bead, by Santa Cruz Biotechnology (1 mentions) Collagen 3, by Abcam (1 mentions) α sma, by Abcam (1 mentions) Tgf β, by Abcam (1 mentions) P mek, by Abcam (1 mentions) P raf, by Abcam (1 mentions) P erk, by Abcam (1 mentions) Collagen 1, by Abcam (1 mentions) Vimentin, by Abcam (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) E cadherin, by Abcam (1 mentions)

2 protocols using recombinant human ccn3

1

Antibody-based Protein Expression Analysis

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Protein A/G beads, anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase, mouse monoclonal or rabbit polyclonal antibodies specific for CCN3 (SC-136967), Twist (SC-81417), N-cadherin (ab76057), E-cadherin (ab40772), p-FAK (SC-11765-R), FAK (SC-932), p-Akt (SC-16646-R), Akt (SC-5298), HIF-1α (ab1), and β-actin (SC-130656) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) or Abcam (Cambridge, UK). Recombinant human CCN3 was purchased from PeproTech (Rocky Hill, NJ, USA). All other chemicals were obtained from Sigma–Aldrich (St Louis, MO).
Chen P.C., Tai H.C., Lin T.H., Wang S.W., Lin C.Y., Chao C.C., Yu H.J., Tsai Y.C., Lai Y.W., Lin C.W, & Tang C.H. (2017). CCN3 promotes epithelial-mesenchymal transition in prostate cancer via FAK/Akt/HIF-1α-induced twist expression. Oncotarget, 8(43), 74506-74518.
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2

Protein Expression and Signaling Pathway Analysis

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Immunohistochemistry and immunoblotting were performed as previously described [8 (link)]. Protein concentrations were determined by the BCA Protein Assay Kit (Beyotime, Shanghai, China). Primary antibodies used for immunohistochemistry and/or immunoblotting were as follow: α-SMA, Collagen I, Collagen III, CCN3, TGF-β, p-RAF, p-MEK, p-ERK, E-cadherin, Vimentin and Actin (Abcam, Cambridge, MA, USA). Recombinant human CCN3 was purchased from Peprotech (Rocky Hill, NJ, USA). TGF-β from LX2 was quantified by ELISA (R&D Labs, Minneapolis, MN, USA). Assays were performed according to the manufacturer’s instructions in quadruplicate as previously described [9 (link)].
Li W., Liao X., Ning P., Cao Y., Zhang M., Bu Y., Lv J, & Jia Q. (2019). Paracrine effects of CCN3 from non-cancerous hepatic cells increase signaling and progression of hepatocellular carcinoma. BMC Cancer, 19, 395.
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