Six- to 8-wk-old C57BL/6 mice were purchased from Japan SLC (Hamamatsu). Slc3a2flox/flox mice crossed with CD4-Cre transgenic mice were generated [14 (link)]. Thy1.1 or CD45.1 C57BL/6 mice and OT-II TCR transgenic mice were purchased from The Jackson Laboratory and Taconic Farms, Inc, respectively. All mice were housed under specific pathogen-free conditions. The studies in this manuscript were approved by the Committee on the Ethics of Animal Experiments of Tokushima University and the care and use of animals complied with institutional guidelines.
Ot 2 tcr transgenic mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
OT-II TCR transgenic mice express a T cell receptor (TCR) specific for the ovalbumin (OVA) peptide presented by the MHC class II molecule I-A^b. These mice are a useful tool for the study of CD4+ T cell responses in the context of antigen presentation and immune system activation.
Automatically generated - may contain errors
Lab products found in correlation
B6 sjl mice, by Jackson ImmunoResearch (3 mentions)
Cd4 cre transgenic mice, by Jackson ImmunoResearch (2 mentions)
Omnibank retroviral gene trapping technique, by Taconic Biosciences (2 mentions)
Tcrb d, by Jackson ImmunoResearch (2 mentions)
Rag1 mice, by Jackson ImmunoResearch (2 mentions)
2d2 tcr transgenic mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6 mice, by Japan SLC (1 mentions)
Cx3cr1 gfp transgenic mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Genentech (1 mentions)
Cd11c cre gfp line, by Jackson ImmunoResearch (1 mentions)
B6 cd45, by Jackson ImmunoResearch (1 mentions)
C57bl 6 background, by Jackson ImmunoResearch (1 mentions)
Wild type c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Ot 1 tcr transgenic mice, by Jackson ImmunoResearch (1 mentions)
Rag1 ko mice, by Jackson ImmunoResearch (1 mentions)
Pten floxed mice, by Jackson ImmunoResearch (1 mentions)
Wild type c57bl 6, by Jackson ImmunoResearch (1 mentions)
Balb c, by Jackson ImmunoResearch (1 mentions)
Female c57bl 6 b6 mice, by Orient Bio (1 mentions)
Balb c mice, by Orient Bio (1 mentions)
22 protocols using ot 2 tcr transgenic mice
1
Generating Transgenic Mice Overexpressing Slc3a2
2
Generating CD11c+ Cell-Specific gp96-Deficient Mice
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CD11c+ cell specific gp96-deficient mice (CD11cCre+Hsp90b1flox/flox) and control littermates (CD11cCre−Hsp90b1flox/flox) were generated by crossing our Hsp90b1flox/flox (gp96 is encoded by Hsp90b1) mice36 (link) with CD11c-cre transgenic mice59 (link). These gp96-deficient mice were further crossed with CX3CR1-GFP transgenic mice46 (link) (Jackson laboratory) to define CX3CR1+ population. OT-II TCR transgenic mice were purchased from Jackson laboratory and bred onto the Ly5.1 background. All animal experimental protocols were approved by the Medical University of South Carolina Institutional Animal Care and Use Committee (IACUC). All methods were carried out in accordance with federal regulation as well as established institutional guidelines and regulations.
3
Mouse Anesthesia and Tissue Harvesting
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Eight- to 16-week-old male and female mice were used for experiments; sexes are recorded in each figure legend. Animals were housed in the Dean McGee Eye Institute's specific-pathogen-free vivarium for at least 1 week prior to experimentation. Wild-type (WT) C57BL/6 and OT-II TCR transgenic mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). Transgenic C57BL/6-background alpha smooth muscle actin GFP-reporter mice (α-SMA-GFP)32 (link) were bred in-house. Transgenic C57BL/6-background mice expressing a floxed vascular endothelial growth factor A allele (Vegfaflox)33 (link),34 (link) were bred within our institution's Rodent Barrier Facility and were originally provided by Genentech (South San Francisco, CA, USA). Mice were anesthetized by intraperitoneal (IP) injection with xylazine (6.6 mg/kg) and ketamine (100 mg/kg) for all procedures and euthanized by exsanguination via intracardiac perfusion with 10 mL PBS prior to tissue collection. The Institutional Animal Care and Use Committee at the University of Oklahoma Health Sciences Center approved all procedures. Experiments were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
4
Transgenic Mouse Models for Immunology
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B6. OT-II TCR-transgenic mice (stock no: 004194) and CD4-Cre–transgenic mice (stock no: 022071) were purchased from The Jackson Laboratory. Rag2–/–/Il2rg–/– mice (Model 4111-F) were obtained from Taconic Biosciences. Traf4fl/fl mice were generated by Cyagen Biosciences using gene-targeting technology. The experimental mice were female and age matched (8–12 weeks). All mice were bred on C57BL/6 background and maintained under specific pathogen–free conditions. All animal experiments were performed with the approval of the Cleveland Clinic’s IACUC.
5
Transgenic Mice for Immunological Studies
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OT-II TCR-transgenic mice (The Jackson Laboratory, Bar Harbor, ME) and C57BL/6N (B6) mice (Kyudo, Saga, Japan) were purchased and kept under specific pathogen-free conditions in the animal facility of Fukuoka Dental College. 6- to 8-week-old mice were used in all experiments. All experiments were performed in accordance with the guidelines of the committee of Ethics of Animal Experiments of Fukuoka Dental College.
6
Generation of FLIP-deficient CD11c+ Mice
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We generated a mouse line with FLIP deficient in CD11c+ cells (CD11c-Flip-KO), aka HUPO mice on a C57BL/6 background, genotyped as Flipflox/floxCD11ccre. HUPO mice are generated by crossing Flipflox/flox mice with CD11c-Cre-GFP transgenic mice (CD11c-Cre-GFP line 4097, Jackson stock 007567).26 (link),27 (link) Control mice were littermates or age and gender matched mice without Flip genomic deletions, genotyping as Flipflox/+CD11ccre or Flipflox/floxCD11c+. We generated HUPO-Rag-/- mice line by crossing HUPO with Rag-/- mice (RAG2 KO, Jackson stock 008449). The HUPO or control mice on C57BL/6 CD45.1+ background were generated by crossing with CD45.1 congenic strain (B6 CD45.1, Jackson stock 002014). OTII TCR-transgenic mice are from Jackson Laboratory (OTII, Jackson stock 004194). All mice were confirmed by PCR genotyping employing genomic DNA extracted from tail biopsies. Also, the CD45.1 or CD45.2 backgrounds were determined by blood flow cytometry employing antibodies to CD45.1 or CD45.2. All mice were breed on the C57Bl/6 background. All mice were bred and maintained in Northwestern University barrier animal facility and all procedures followed ethical guidelines and approved by Northwestern IACUC.
7
Genetically Modified Mice for Immunological Studies
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C57BL/6 wild-type mice and OTII TCR transgenic mice (C57BL/6 background) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Vstm5-knockout mice were generated by Macrogen using CRISPR/Cas9 (Seoul, Korea). LFA-1-knockout mice were provided by Dr. Minsoo Kim (University of Rochester, NY, USA). TLR4-knockout mice were provided by Dr. Sang-Myeong Lee (Chonbuk National University, Iksan, Korea). All mice were housed in specific pathogen-free conditions. All experimental methods and protocols were approved by the Institutional Animal Care and Use Committee of the School of Life Sciences, Gwangju Institute of Science and Technology (Gwangju, Korea) and performed in accordance with their approved guidelines.
8
Senp7-floxed Mice and T Cell Regulation
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Senp7-floxed mice were generated at the GemPharmatech using a LoxP-targeting system. The Senp7-floxed mice were crossed with Cd4-Cre transgenic mice (The Jackson Laboratory) to produce age-matched Senp7fl/fl and Senp7fl/flCd4-Cre mice. The Pten-floxed mice (The Jackson Laboratory) were crossed with Senp7fl/flCd4-Cre mice to produce Senp7+/+Ptenfl/flCd4-Cre (Pten-KO) and Senp7fl/flPtenfl/flCd4-Cre (DKO) mice. Rag1-KO mice, OT-I TCR–transgenic mice, OT-II TCR–transgenic mice, and B6.SJL mice were from The Jackson Laboratory. Male and female mice were sex matched and used at 6 to 10 weeks of age. Mice were maintained in a specific pathogen–free (SPF) facility at the Shanghai Jiao Tong University School of Medicine.
9
Murine Models for Immunological Studies
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C57BL/6 wild-type, BALB/c, OTI, and OTII TCR transgenic mice (C57BL/6 background) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). All mice were housed in specific pathogen-free conditions. All experimental methods and protocols were approved by the Institutional Animal Care and Use Committee of the School of Life Sciences, Gwangju Institute of Science and Technology, and performed in accordance with their approved guidelines. In general, sex-matched, 8-week-old mice were used.
10
Mouse Models for Immunological Studies
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Female C57BL/6 (B6) mice and BALB/c mice were purchased from Orient Bio (Seoul, Korea). OT-II TCR transgenic mice and IL-6 KO mice (B6 background), were from the Jackson Laboratory (Bar Harbor, ME). Mice were maintained under specific pathogen-free condition and were used between 6 and 10 weeks of age. All animals were handled in strict accordance with good animal practice as defined by the relevant national and/or local animal welfare bodies, and all animal work was approved by Ewha Womans University’s institutional animal care and use committee (IACUC, Approval Number.15-069).
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