PAH quantification standard consisted of 19 parent PAHs including the 16 US EPA PAHs and benzo(e)pyrene, perylene, and dibenzothiophene. 2-methylphenanthrene, 3,6-dimethylphenanthrene, 1,2,6-trimethylphenanthrene, and 1,2,6,9-tetramethylphenanthrene were used to quantify alkylated phenanthrenes. For a full list of PAHs used for quantification and the corresponding deuterated internal standards see Table A.1 (supplementary data). All PAHs and deuterated PAHs were acquired from CIL (Cambridge, UK, purity >97%). From the mixture of unlabeled PAHs, seven calibration solutions were prepared in isooctane (pro analysis grade, Merck) in the concentration range 3-300 ng/mL. The mixture of deuterated internal standards was prepared in isooctane and had a concentration of each compound of 300 ng/mL. The PAH quantification standard and deuterated internal standard mixture were further diluted 20 times in ethyl acetate (>99.7%, Sigma-Aldrich) before addition of 200 μL to each of the 4 mL extracts. The final internal standard concentration was 0.75 ng/mL.
Isooctane
Manufactured by Merck Group
Sourced in Germany, United States, Italy, Belgium, Poland
Isooctane is a colorless, volatile hydrocarbon liquid used as a reference standard and solvent in various analytical applications. It has a high purity and is primarily used for calibrating and testing laboratory equipment.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform, by Merck Group (18 mentions)
Methanol, by Merck Group (16 mentions)
N hexane, by Merck Group (13 mentions)
Ethanol, by Merck Group (9 mentions)
Potassium iodide, by Merck Group (9 mentions)
Acetic acid, by Merck Group (9 mentions)
Hydrochloric acid (hcl), by Merck Group (8 mentions)
Toluene, by Merck Group (7 mentions)
Acetone, by Merck Group (7 mentions)
P anisidine, by Merck Group (7 mentions)
Potassium hydroxide, by Merck Group (6 mentions)
Ethyl acetate, by Merck Group (6 mentions)
Diethyl ether, by Merck Group (6 mentions)
Methanol, by Thermo Fisher Scientific (6 mentions)
Sodium hydroxide (naoh), by Merck Group (6 mentions)
Folin ciocalteu reagent, by Merck Group (5 mentions)
Hexane, by Merck Group (5 mentions)
Dichloromethane dcm, by Merck Group (5 mentions)
Sodium chloride (nacl), by Merck Group (5 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (5 mentions)
74 protocols using isooctane
1
Polycyclic Aromatic Hydrocarbon Quantification
2
Chemically Profiling Turkish CS Seeds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CS seeds used in the study were collected from different locations, at different altitudes, longitudes and latitudes, as determined by a Global Positioning System (GPS), at their maturation stage from June to August, 2017, according to their maturation levels. Samples were collected from the provinces of Mardin, Van, Gaziantep, Bitlis, Erzincan, Diyarbakır, Ağrı, Şanlıurfa, Siirt, Muş and Batman, all located in Turkey (Figure 1). Three groups of samples, each consisting of fifteen plants, were collected per location. Seed samples were coded as CS 1, CS 2 …, and CS 11 , according to the locations from where they were collected, as given in Table 1. Folin-Ciocalteu's reagent, methanol, n-hexane, isooctane, potassium persulfate methanol, isooctane, potassium persulfate, α-, β-, γ-and δ-tocopherol standards were obtained from Merck (Darmstadt, Germany). 2,2-diphenyl-1picrylhydrazyl (DPPH), 2,2+-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, 5-methyl 2 hexanone, trolox and standards of fatty acid methyl esters (37 FAME mix) were obtained from Sigma Chemical Co. (Sigma-Aldrich GmbH, Sternheim, Germany).
3
Synthesis of Metal-Organic Nanostructures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zn(NO3)2·6H2O and Al(NO3)3·9H2O were purchased by Riedel-de-Haën (Selze, Germany). AgNO3 by Carlo Erba (Milan, Italy), cetyltrimethylammonium bromide (CTABr), n-butanol, isooctane, NH3, cefazolin, and nalidixic acid are a Sigma-Aldrich (Darmstadt, Germany) product. Deionized water was obtained by a reverse osmosis process using a Milli Q system (Millipore, Rome, Italy).
4
Fatty Acid Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were used as received. Iso-octane was acquired from Sigma-Aldrich (St. Louis, MO, USA) and Folin–Ciocalteu’s reagent from VWR International (Rosny-sous-Bois, Paris, France). Standard gallic acid compound was acquired from Sigma-Aldrich (St. Louis, MO, USA). Double-deionized water was obtained with a Milli-Q water purification system (Millipore, Bedford, MA, USA). Potassium hydroxide pellets were supplied by Merck (Darmstadt, Germany) and n-heptane and methanol were purchased from VWR International (Fontenay-sous-Bois, Paris, France). Supelco 37-Component FAME mix, used as a standard solution for identification of FA-components, was purchased from Sigma-Aldrich (Bellefonte, PA, USA). H and air for GC were supplied by Nippon Gases Portugal.
5
Chitosan-Based Nanoparticle Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan powder with a degree of deacetylation about 89% was purchased from Ruji Biotechnology Co., Ltd. (Shanghai, China). All of the chemical reagents including KOH, LiOH·H2O, urea, NaOH, HCl, CoCl2, epichlorohydrin, chloroform, methylbenzene acetone anhydrous DMSO, alcohol and NH3·H2O were analytical grade from commercial sources in China. Tween-80, Span-80, glutaraldehyde, toluene and isooctane were supplied by Sigma-Aldrich Company. NaCl, EDTA·2Na and acetic acid were obtained from VWR International.
6
Fatty Acid Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lipids extracted from LM were methylated according to the method by Lepage and Roy (1986) (link) and the fatty acid composition was analyzed using gas chromatography. Approximately 200 μL of each lipid extract were converted into fatty acid methyl esters by adding 2 mL methanol:benzene (4:1, vol:vol) solution and 200 μL of acetyl chloride, and heated for 40 min at 100°C on a heating block (Lab & Tools, Anyang, Korea). One mL isooctane (Sigma-Aldrich, Saint Louis, MO, USA) and 8 mL of 6% potassium carbonate (Junsei, Tokyo, Japan) were added, and the solution was centrifuged (1580R, Labogene, Daejeon, Korea) for 10 min at 500×g. One (1) μL of the supernatant was subjected to analysis with a gas chromatography (Clarus 500; Perkin Elmer, Shelton, CT, USA) equipped with a fused column SP 2560 (100 m in length, 0.25 mm in diameter, 0.2 um film thickness) using nitrogen as the carrier gas (flow rate of 1.0 mL/min). The initial oven temperature was 210°C; the injector and detector temperatures were 240°C and 250°C, respectively.
7
Stability of Milk Powder under Antioxidants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Skim milk powder was collected from a local dairy company, and the same batch was used for the production of all FFMPs. Antioxidant-free refined sunflower oils were purchased from a local store (SuperValu, Dublin, Ireland), and stored in closed containers, in the dark under refrigeration (4 °C) until use.
1,1,3,3-tetraethoxypropane (TEP), trichloroAcetic acid (TCA), starch, potassium iodide, 37% hydrochloric acid (HCl), isooctane, BHA, BHT, rosmarinic acid, 1-pentanol, 2-heptanone, 2-pentanone, 3-octen-2-one, 2, 4-decadienal, hexanal, heptanal, octanal, pentanal, 2-nonenal (E), acetone, 2-nonanone, undecanal 2-methyl-4-pentanol, 2-methyl-3-heptanone, isovaleraldehyde and ethyl butyrate were purchased from Sigma-Aldrich (now Merck, Wicklow, Ireland), whereas carnosol and carnosic acid were obtained from Extrasynthese (Extrasynthese Co., Genay Cedex, France). Thiobarbituric acid (TBA) and sodium thiosulfate were obtained from VWR (VWR International Ltd., Dublin, Ireland). Isopropanol (PrOH) was acquired from Romil (Lennox Laboratory Supplies LTD, Dublin, Ireland) and n-hexane from Honeywell (Honeywell, Dublin, Ireland). Acetic acid was obtained from AppliChem GmbH (Darmstadt, Germany). Milli-Q® water (H2O) (18 mΩ) (Merck Millipore, Molsheim, France) or deionized H2O was used throughout the experiments.
1,1,3,3-tetraethoxypropane (TEP), trichloroAcetic acid (TCA), starch, potassium iodide, 37% hydrochloric acid (HCl), isooctane, BHA, BHT, rosmarinic acid, 1-pentanol, 2-heptanone, 2-pentanone, 3-octen-2-one, 2, 4-decadienal, hexanal, heptanal, octanal, pentanal, 2-nonenal (E), acetone, 2-nonanone, undecanal 2-methyl-4-pentanol, 2-methyl-3-heptanone, isovaleraldehyde and ethyl butyrate were purchased from Sigma-Aldrich (now Merck, Wicklow, Ireland), whereas carnosol and carnosic acid were obtained from Extrasynthese (Extrasynthese Co., Genay Cedex, France). Thiobarbituric acid (TBA) and sodium thiosulfate were obtained from VWR (VWR International Ltd., Dublin, Ireland). Isopropanol (PrOH) was acquired from Romil (Lennox Laboratory Supplies LTD, Dublin, Ireland) and n-hexane from Honeywell (Honeywell, Dublin, Ireland). Acetic acid was obtained from AppliChem GmbH (Darmstadt, Germany). Milli-Q® water (H2O) (18 mΩ) (Merck Millipore, Molsheim, France) or deionized H2O was used throughout the experiments.
8
Antioxidant Activity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethanol (Sigma–Aldrich; Merck KGaA, Darmstadt, Germany), 1,1-diphenyl-2-picrylhydryl (DPPH, Sigma–Aldrich, Taufkirchen, Germany), isooctane (Sigma–Aldrich Chemie GmbH, Munich, Germany), glacial acetic acid (Sigma–Aldrich Chemie GmbH, Munich, Germany), benzene (Sigma–Aldrich Chemie GmbH, Munich, Germany), thiobarbituric acid (Sigma–Aldrich Chemie GmbH, Munich, Germany), PBS isotonic solution (RemedLab, Bucharest, Romania) and egg albumin (Oxford Lab Fine Chem, Maharashtra, India).
All reagents used for chemical analysis were purchased from Sigma–Aldrich Chemie GmbH (München, Germany) and Geyer GmbH (Renningen, Germany) and were of analytical quality.
All reagents used for chemical analysis were purchased from Sigma–Aldrich Chemie GmbH (München, Germany) and Geyer GmbH (Renningen, Germany) and were of analytical quality.
9
Tryptophan Quantification in Infected Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At 48 hpi, infected cells were pelleted and resuspended in perchloric acid (Sigma-Aldrich) (10% wt/vol) solution containing 1 mM diethylenetriamine pentaacetate (Sigma-Aldrich) for cell lysis, followed by 4°C centrifugation at 1,500 rpm. Tryptophan concentrations were measured in supernatants using high-performance liquid chromatography-linked tandem mass spectrometry (HPLC-MS/MS) with the EZ-FAAST aa derivatization kit (Phenomenex, Torrance, CA) as previously described (70 (link)). Briefly, supernatants containing tryptophan were mixed with an internal standard (tyrosine 3,3-D2), and pellets were mixed with a strong cation exchange resin (Phenomenex, Torrance, CA). Derivatization was performed using the manufacturer’s protocols. Derivative products were extracted with isooctane (Sigma-Aldrich), dried under nitrogen, and reconstituted with a mobile phase. Analytes were quantified based on purified standard compounds. Data were normalized against Ct 16S rRNA gene transcription levels.
10
Synthesis of Semiconductor Nanocrystals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cadmium oxide (CdO, 99.99%, trace metals), trioctylphosphine (TOP, 90% technical grade), sulfur (S, 99.98%), 1-octadecene (ODE, 90%, technical grade), iso-Octane (99.7%, HPLC grade), 1-Octanethiol (> 98.5%), trimethylammonium chloride (TMACl, > 98%), potassium hydroxide (KOH, 99.99%), dimethyl sulfoxide (DMSO, > 99.9%) magnesium acetate tetrahydrate (99%), zinc acetate dihydrate (> 98%), lithium acetate (99.95%) and 1-butanol (anhydrous, 99.8%) were purchased from Sigma-Aldrich. Zinc acetate anhydrous (+ 99.9%) and ethyl acetate (> 99.5%, ACS certified) were purchased from Thermoscientific. Selenium (Se, 99.999%, metals basis) and oleic acid (90%, technical grade) were purchased from Alfa-Aesar. Octane (+ 99%, extra pure) was purchased from Acros Organics. All the reagents were used as received. Poly(ethylene dioxythiophene): polystyrene sulfonate (PEDOT: PSS) was purchased from Ossila. Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4’-(N-(p-butylphenyl)) diphenylamine)] (TFB) was purchased from American Dye Source. Polyvinylpyrrolidone (PVP10) with an average molecular weight of 10,000 was purchased from Sigma-Aldrich. Patterned ITO-glass substrates with 15 Ω resistance and 25.4 mm × 25.4 mm × 0.7 mm were purchased from Luminescence Technology Corp.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!