Aceton

Manufactured by Merck Group

Sourced in Italy, Germany

Acetone is a clear, colorless liquid that is used as a solvent in various laboratory applications. It is a common component found in many chemical and scientific products. Acetone has a distinct, characteristic odor and is highly volatile.

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Lab products found in correlation

6 protocols using aceton

Spectrophotometric Assay of Oxidative Enzymes

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Dimethylformamide (DMF), trichloracetic acid (TCA), horseradish peroxidase (HRP) (type VI, ≥250 units/mg), catalase (from bovine liver, 2,000–5,000 units/mg), 4-aminoantipyrine (4-AP), phenol, graphene oxide (GO), activated charcoal, agar powder, potassium chloride, silver nitrate, potassium hexachloroplatinate (IV), gold(III) chloride trihydrate, aceton, 2-isopropanol, ethanol, and other chemicals were purchased from Sigma-Aldrich (St. Louis, United States). All reagents were of analytical grade. Mn(III) meso-tetra(N-methyl-4-pyridyl) porphine pentachloride (MnTMPyP) and Mn(III) meso-tetra(4-pyridyl) porphine chloride (MnTPyP) (>95%) were obtained from Frontiers Scientific Inc. Phosphate buffer (PB) solutions (0.1 M, pH 7.4) were prepared from disodium hydrogen phosphate and sodium dihydrogen phosphate. The pH of the solutions was controlled by the pH-meter 765 (Knick GmbH). All solutions were prepared using a Milli-Q water purification system (Merk KGaA, Germany).

Mourzina Y.G., Ermolenko Y.E, & Offenhäusser A. (2021). Synthesizing Electrodes Into Electrochemical Sensor Systems. Frontiers in Chemistry, 9, 641674.

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Ultrastructural Analysis of Biological Samples

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Sample primary fixation was done by 4% PFA, 2% Glutaraldehyde (Carl Roth #4157) in 0.1M Cacodylate Buffer (Science Services #11650). For transmission electron microscopy (TEM), the tissue was then post-fixed in 0.5% Osmium tetroxide (Science Services #E19150) in ddH₂O for 60min on ice and then washed 6x in ddH₂O. The tissue was incubated in 1% aqueous Uranyl acetate solution (Science Services #E22400–1) for 2h in the dark and washed 2x in ddH₂O. Dehydration was performed by 15min incubation steps in 30%, 50%, 70%, 90% and 2× 100% Ethanol (Fisher Scientific #32205) and 2× 100% Aceton (Sigma-Aldrich #179124). After embedding in Durcupan resin (Sigma-Aldrich #44611 and #44612), ultrathin sections (55nm) were performed using a UC7 Ultramicrotome (Leica), collected on Formvar-coated (Science Services #E15830–25) copper grids (Plano #G2500C). Post-staining was done for 1min with 3% Lead Citrate (Delta Microscopies #11300). For scanning electron microscopy (SEM), the fixated samples were dehydrated in 70%, 80%, 90% and 100% Ethanol (each step for 1h at RT) and incubated in a 1:1 solution of Ethanol and Hexamethyldisilazan (HMDS; Carl Roth #3840.2) for 30min. After incubation in 100% HMDS, the solvent was allowed to evaporate. The dehydrated tissue was mounted onto sample holders and sputtered with gold using a Polaron Cool Sputter Coater E 5100.

Tasca A., Helmstädter M., Brislinger M., Haas M., Mitchell B, & Walentek P. (2021). Notch signaling induces either apoptosis or cell fate change in multiciliated cells during mucociliary tissue remodeling. Developmental cell, 56(4), 525-539.e6.

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Synthesis and Characterization of PHEA-Based siRNA Delivery System

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Triethylamine (TEA), Bis(4-nitrophenyl)carbonate (BNPC), anhydrous N,N’-dimethylformamide (a-DMF), 1,2-Bis(3-aminopropylamino)ethane (bAPAE), O-(2-Aminoethyl)-O’-methyl poly(ethylene glycol) 2000 (H₂N-PEG₂₀₀₀) (0.4 mmol NH₂/g), disuccinimidylcarbonate (DSC), dichloromethane, aceton, diethylether, 2,4,6-Trinitrobenzenesulfonic acid (TNBS), agarose, ethidium bromide, mucine from porcin stomach were purchased from Sigma-Aldrich (Milan, Italy). All used reagents were of analytic grade.
Duplexed siRNA were purchased from Biomers.net (Ulm, Germany). The gene target sequences (5′→3′) are: CAGUUCCGCCACUUGCCAA (sense), UUGGCAAUGGCGGAACUG (antisense).
α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) was synthetized via polysuccinimide (PSI) reaction with ethanolamine in DMF solution, and purified according to a previously reported procedure [11 (link)].
¹H-NMR (300 MHz, D₂O, 25°C, TMS): δ 2.71 (m, 2H_PHEA, –COCHCH₂CONH–), δ 3.24 (m, 2H_PHEA, –NHCH₂CH₂O–), δ 3.55 (m, 2H_PHEA, –NHCH₂CH₂OH), δ 4.59 [m, 1H_PHEA, –NHCH(CO)CH₂–].

Craparo E.F., Drago S.E., Mauro N., Giammona G, & Cavallaro G. (2020). Design of New Polyaspartamide Copolymers for siRNA Delivery in Antiasthmatic Therapy. Pharmaceutics, 12(2), 89.

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Immunofluorescence Analysis of Mouse Skin Samples

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Punch biopsies were taken from harvested mouse skin and frozen at –20°C. Sections of 8 μm were generated on glass microscope slides (Star Frost, Laborchemie GmbH, Vienna, Austria) with a cryotome (MICROM HM 550, Thermo Fisher Scientific Inc., Runcorn, UK). For histological analysis sections were stained with hematoxylin (Merck) and eosin (Merck). For immunofluorescence staining sections were fixed in ice cold aceton (Merck) for 2 min and blocked in 0.5% BSA (Sigma-Aldrich) in PBS (Invitrogen) for 60 min. Primary antibodies were: pAb against the NC-1 domain of type VII collagen (LH7.2, kindly provided by Dr. Alexander Nyström from Freiburg) produced in rabbit diluted 1:3000 in PBS for 90 min, monoclonal anti-involucrin (Santa Cruz Biotechnology, Heidelberg, Germany), 1:100 for 2 h. The secondary antibody anti-rabbit AF594 and anti-mouse AF488 (Invitrogen) were incubated for 1 h in a 1:400 dilution in PBS in the dark. DAPI (Sigma-Aldrich) staining of the nuclei (Sigma-Aldrich) was performed in dilution of 1:2000 for 10 min. After mounting, using a fluorescence aqueous mounting medium (Dako), the sections were observed under the confocal laser scanning unit (Axio Observer Z1 attached to LSM710, Zeiss) and images were converted to TIFF files with the ZEN Blue 2012 (Carl Zeiss) software.

Peking P., Koller U., Duarte B., Murillas R., Wolf S., Maetzig T., Rothe M., Kocher T., García M., Brachtl G., Schambach A., Larcher F., Reichelt J., Bauer J.W, & Murauer E.M. (2017). An RNA-targeted therapy for dystrophic epidermolysis bullosa. Nucleic Acids Research, 45(17), 10259-10269.

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Fabrication and Characterization of Photocatalytic Composites

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The commercial TiO₂ (mainly anatase grade) and Al₂O₃ (neutral activity I-II) were purchased from MERCK KGaA (Germany). The locally available kaolinite precursors were procured from Bijoypur clay. NaOH pellets, hydrochloric acid (37 %) and buffer solutions (pH 4 and pH 7) were purchased from Active Fine Chemicals Ltd., Dhaka, Bangladesh. Aceton, m ethanol and ethanol were procured from Merck KGaA, Germany. Ascorbic acid (C₆H₈O₆), 2-propanol ((CH₃)₂CHOH), and diammonium oxalate monohydrate ((NH₄)₂C₂O₄·H₂O) were also purchased from Merck KGaA, Germany. Methylene blue (MB) (CAS-122965-43-9) was purchased from Sigma-Aldrich (Merck), and Lab-grade Remazol Red (RR) dye was purchased from local dye suppliers of the textile industry. The stock solutions of MB and RR were prepared using purified deionized water obtained from a UV water purification system, procured from Labtron Equipment Ltd., UK. All chemicals and reagents were utilized without further purification.

Lata N.P., Hussain M.S., Abdulla-Al-Mamun M., Rashid T.U, & Shamsuddin S.M. (2024). Fabrication and synergistically enhanced photocatalytic activity of ternary kaolinite, TiO2, and Al2O3 (K65T30A5) nanocomposite for visible-light-induced degradation of methylene blue and remazol red dye. Heliyon, 10(8), e29255.

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Cellulose Acetate Functionalization Protocols

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Cellulose acetate (CA) with 39. 7 wt% acetyl content, corresponding to the degree of substitution of 2.4, and an average Mn of 50 kDa was purchased from Sigma-Aldrich. CA powder was dried under vacuum at 100 °c for 48 h to remove the sorbed moisture prior to use. 4-Chlorobu tyryl chloride (98%) was purchased from Across Organics. N-methyl imidazole (> 99%), N-methylpyrrolidine (> 99%), 2dimethylamino ethanol (99%), and triethylamine (99%) were pur chased from Sigma-Aldrich. Dichloromethane (99.5%) was purchased from Scharlab. Bis(trifluoromethylsulfonyl)imide lithium salt (LiTf 2 N) was purchased from IoLiTec.
Matrimid® 9725 was kindly provided by Huntsman (Switzerland) and the non-woven polypropylene/polyethylene (PP/PE) fabric Nova texx® 2483 by Freudenberg (Germany). P-xylylenediamine (XDA, > 98%) cross-linker was purchased from Fluka. N-methylpyrrolidinone (NMP, Acros, 99%), tetrahydrofuran (THF, Acros, 99.5%), aceton (Merck, 99.8%), ethanol (Fisher Scientific, 99.5%), methanol (Acros, 99.8%), isopropanol (IPA, VWR, 99.5%), n-hexane (Merck, 99%) were all used without further purication. Polydimethylsiloxane (PDMS) was acquired from Momentive Performance Materials in the form of RTV615 silicone rubber compound kit (Leverkusen, Germany).

Nikolaeva D., Verachtert K., Azcune I., Jansen J.C, & Vankelecom I.F.J. (2021). Influence of ionic liquid-like cationic pendants composition in cellulose based polyelectrolytes on membrane-based CO₂ separation. Carbohydrate polymers, 255.

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