3 16pk centrifuge system

The chromatographic analysis was performed using an Agilent 1220 Infinity HPLC-UV system (Agilent Technologies, Santa Clara, CA, USA). The system comprised of: (i) the degasser, (ii) the column oven, (iii) the manual injector, and (iv) the UV detector. The OpenLAB software (Agilent Technologies, Santa Clara, CA, USA) and the Method and Run control package were employed to monitor the analysis. The sonication of the samples was performed in a MRC:DC-150-H ultrasonic bath provided by MRC (Essex, UK). A vortex mixer purchased from VELP Scientifica (Usmate Velate, Italy) was used for agitation. Centrifugation was carried out using a 3-16PK centrifuge system supplied by Sigma (Osterode am Harz, Germany). Prior to the injection in the chromatographic system, all samples were filtered through QMax RR 25 mm 0.22 μm PTFE syringe filters purchased from Frisenette ApS (Knebel, Germany).

A 1220 Infinity HPLC-UV system from Agilent Technologies (Santa Clara, CA, USA) was used for the chromatographic analysis of the phenolic compounds and tocopherols. The HPLC system consisted of a degasser, a column oven, a manual injector, and a UV detector. For monitoring the analysis, the OpenLAB software (Agilent Technologies, Santa Clara, CA, USA) was used with the Method and Run Control package. For peak identification and data processing, the Data Analysis software package (Agilent Technologies, Santa Clara, CA, USA) was used. QMax RR syringe filters (0.22 µm Nylon; and 0.22 PTFE) were acquired from Frisenette ApS (Knebel, Denmark) to filter the samples prior to analysis. For agitation, a vortex mixer from VELP Scientifica (Usmate Velate, Italy) was used. Centrifugation was carried out using a 3-16PK centrifuge system from Sigma (Osterode am Harz, Germany). For the green extractions, a MARS X Model 1000 microwave oven (CEM, Matthews, NC, USA) equipped with a 14-position holder, PTFE vessels, and a stirring mechanism was used.