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Pentobarbital sodium

Manufactured by Guangzhou Chemical
Sourced in Japan

Pentobarbital sodium is a barbiturate compound commonly used as a sedative and hypnotic drug. It is a white, crystalline powder that is soluble in water. Pentobarbital sodium is utilized in various laboratory and research applications, but its specific functions and uses should be determined by the appropriate professionals.

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2 protocols using pentobarbital sodium

1

Quantitative PCR and Immunohistochemistry Protocol

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Pure AST is provided by Shanghai Yuanye Biotechnology Co., Ltd. In the experiment, pure AST was dissolved with phosphate buffer (PBS) and diluted with saline. Pentobarbital sodium, papain, and cysteine were purchased from Guangzhou Chemical Reagent Factory. Hematoxylin, water-soluble eosin Y, toluidine blue O, paraformaldehyde, and Ethylene Diamine Tetraacetic Acid (EDTA) were from TAKARA, Japan. Anti- PEG2, β-actin, and other monoclonal antibodies were purchased from Abcam, UK. Finally, the immunohistochemical kit was purchased from Boster, Wuhan, China) The fluorescence quantitative PCR detection kit was purchased from Baoshi, Dalian, China, and the Prime Script RT kit were purchased from Takara, Dalian, China.
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2

Western Blot Analysis of ERβ Signaling

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Taq Master Mix (SinoBio, Shanghai, China), agarose (Biowest, Nuaille, France), sterile double distilled water, sterile double distilled water, monoclonal rabbit β-actin antibody (dilution, 1:5,000; cat. no. MA5-15739; Invitrogen Life Technologies, Carlsbad, CA, USA), polyclonal rabbit ERβ antibody (dilution, 1:500; cat. no. BD-PT1637; BioLegend, Inc., San Diego, CA, USA), monoclonal rabbit phosphorylation ERβ antibody (dilution, 1:1,000; cat. no. orb10614; Cell Signaling Technology, Inc., Danvers, MA, USA), 0.9% stroke-physiological saline solution (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), pentobarbital sodium (Guangzhou Chemical Reagent Factory, Guangzhou, China), TRIzol (Invitrogen Life Technologies), AngII (RayBiotech, Norcross, GA, USA), and acetic acid (Guangzhou Chemical Reagent Factory) constituted the reagents used in this study. Phosphate buffer was purchased from Gibco Life Technologies (Carlsbad, CA, USA).
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