N isopropylacrylamide

N-Isopropylacrylamide (≥ 98%, NIPAm) was acquired from Polysciences Inc. (Warrington, PA, USA). Dialysis membrane tubing was purchased from Spectrum Laboratories (Dominguez, CA). (1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (≥ 98%, HBTU), N-hydroxybenzotriazole (> 97%, HOBT), N,N′-methylenebisacrylamide (99%, MBA), sodium dodecyl sulfate (SDS; 10% w/v in water), 2-acrylamido-2-methyl-1-propanesulfonic acid (99%, AMPSA), dithiothreitol (98%, DTT), fluorescein o-acrylate (98%, F), N,N-Diisopropylethylamine (99%, DIPEA), potassium persulfate (99%, K₂S₂O₈), N,N′-bis(acryloyl)cystamine (98%, BAC) and dimethyl sulfoxide (DMSO) were acquired from Sigma-Aldrich (St. Louis, MO, USA). N,O-dimethacryloyl hydroxylamine (98%, DMHA) were synthesized based on a previous protocol^{30 (link)}. NIPAm, MBA, and AMPSA were stored under nitrogen at 4 °C. All water used in synthesis, dialysis, and testing was treated by a Milli-Q system (Millipore, Billerica, MA, USA; 18.2 MΩ·cm resistivity). Acrylate-PEG₂₀₀₀ was purchased from Laysan Bio (AL).

N-Isopropylacrylamide (NIPAm, ≥98%) was acquired from Polysciences Inc. (Warrington, PA). Dialysis membrane tubing was purchased from Spectrum Laboratories (Dominguez, CA). (1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (≥98%, HBTU), N-hydroxybenzotriazole (>97%, HOBT), N,N’-methylenebisacrylamide (99%, MBA), sodium dodecyl sulfate (SDS; 10% w/v in water), 2- acrylamido-2-methyl-1-propanesulfonic acid (99%, AMPSA), dithiothreitol (98%, DTT), fluorescein o-acrylate (98%), N,N-Diisopropylethylamine (99%, DIPEA), potassium persulfate (99%, K₂S₂O₈), N,N’-bis(acryloyl)cystamine (98%, BAC), trifluoroacetic acid and dimethyl sulfoxide (DMSO) were acquired from Sigma-Aldrich (St. Louis, MO). NIPAm, MBA, and AMPSA were stored under nitrogen at 4°C. Acrylate-PEG2000 was purchased from Laysan Bio (Arab, AL). Triisopropyl silane was purchased from TCI America (Boston, MA), and ethane dithiol was purchased from Alfa Aesar (Ward Hill, MA). All water used in synthesis, dialysis, and testing was treated by a Millipore Milli-Q system (Billerica, MA; 18.2 MΩ•cm resistivity).