A Malvern ZetaSizer Nano-ZS analyser (Malvern Instruments, UK) was used for dynamic light scattering (DLS) measurements. The 4 mW HeNe laser was set at λ = 633 nm with the detector angle at 173° for backscattering measurements. The NISTmAb samples were adjusted with 25 mmol/L His-buffer (pH 6.0) to give a concentration of 1 mg/mL. His-buffer was filtered through a Millipore Millex-GV 0.22 μm PVDF filter. DLS spectra were acquired in a disposable polystyrene ultra-micro cuvette at room temperature. Each spectrum was collected over three runs consisting of twelve ten second scans. The three runs were then averaged.
Millex gv 0.22 μm pvdf filter
Manufactured by Merck Group
Sourced in Morocco
The Millex-GV 0.22 μm PVDF filter is a laboratory filtration device designed to remove particulate matter from liquid samples. It features a polyvinylidene fluoride (PVDF) membrane with a pore size of 0.22 micrometers, which can effectively filter out a wide range of microorganisms and particulates while allowing the liquid to pass through.
Automatically generated - may contain errors
3 protocols using millex gv 0.22 μm pvdf filter
1
Dynamic Light Scattering of NISTmAb
2
Dynamic Light Scattering of Protein Samples
3
Formulation and Stability Assessment of Lyophilized Myoglobin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mb was solubilized and dialyzed
thoroughly in 2.5 mM potassium phosphate buffer, pH 7.0 at 4 °C.
The dialyzed Mb stock solution (6.8 mg/mL) was used to prepare the
formulations shown in Table1 . The samples
were filtered using Millex-GV 0.22 μm PVDF filter (Millipore,
Billerica, MA) and filled as 2.2 mL into 8 mL glass vial (Nuova Ompi,
Padova, Italy) using a peristaltic pump (Flexicon PF6, Flexicon, Wilmington,
MA). The vials were loaded and freeze-dried in a Boc Edwards Lyophilizer
(Lyomax 0.4) (Boc Edwards, Tonawanda, NY). Lyophilization was initiated
by precooling the shelves to −2 °C, followed by freezing
at −40 °C. Primary drying was carried out under vacuum
(70 mTorr) at −35 °C for 75 h followed by secondary drying
at 25 °C for 12 h and 5 °C for 12 h. All vials were backfilled
with nitrogen prior to sealing. After lyophilization, the Mb formulations
were analyzed for any excipient and/or lyophilization induced changes
using dynamic light scattering (DLS), size exclusion chromatography
(SEC), FTIR, and ssHDX-MS, as described below. The lyophilized samples
were then placed on stability at 25 °C and 60% RH or 40 °C
and 75% RH over a period of 1 year. Samples were withdrawn at intervals
and analyzed for aggregate content by SEC and DLS as described below.
thoroughly in 2.5 mM potassium phosphate buffer, pH 7.0 at 4 °C.
The dialyzed Mb stock solution (6.8 mg/mL) was used to prepare the
formulations shown in Table
were filtered using Millex-GV 0.22 μm PVDF filter (Millipore,
Billerica, MA) and filled as 2.2 mL into 8 mL glass vial (Nuova Ompi,
Padova, Italy) using a peristaltic pump (Flexicon PF6, Flexicon, Wilmington,
MA). The vials were loaded and freeze-dried in a Boc Edwards Lyophilizer
(Lyomax 0.4) (Boc Edwards, Tonawanda, NY). Lyophilization was initiated
by precooling the shelves to −2 °C, followed by freezing
at −40 °C. Primary drying was carried out under vacuum
(70 mTorr) at −35 °C for 75 h followed by secondary drying
at 25 °C for 12 h and 5 °C for 12 h. All vials were backfilled
with nitrogen prior to sealing. After lyophilization, the Mb formulations
were analyzed for any excipient and/or lyophilization induced changes
using dynamic light scattering (DLS), size exclusion chromatography
(SEC), FTIR, and ssHDX-MS, as described below. The lyophilized samples
were then placed on stability at 25 °C and 60% RH or 40 °C
and 75% RH over a period of 1 year. Samples were withdrawn at intervals
and analyzed for aggregate content by SEC and DLS as described below.
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