Carisoprodol

Meprobamate, carisoprodol, pentobarbital, diazepam, THIP (4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol), salts and buffers were purchased from Sigma Aldrich (St. Louis, MO). GABA was obtained from Acros Organics (New Jersey, US). Bemegride was obtained from Pfaltz & Bauer, Inc. (Waterbury, CT). Stock solutions of Meprobamate, pentobarbital and carisoprodol were made using dimethylsulfoxide (DMSO). Drugs were diluted in normal saline, so that the final DMSO concentration (vol/vol) of the test solutions was ≤0.3%. GABA, diazepam and bemegride stock solutions were prepared using de-ionized H₂O. All stock solutions were stored at −20 °C. On experimental days, drug-containing solutions were prepared from stock by serial dilution into external solution.

Carisoprodol, pentobarbital, THIP (4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol), salts and buffers were purchased from Sigma Aldrich (St. Louis, MO). GABA was obtained from Acros Organics (New Jersey, US). GABA stock solution (500 mM) was prepared in de-ionized water. A stock solution (1 or 3M) of Carisoprodol and THIP (1M) was made in DMSO. Final concentrations of DMSO in working solutions were 0.3% or less, a concentration that does not affect GABA-gated current. Diazepam and furosemide were utilized from a stock solution prepared in water. All stock solutions were stored at −20C. On experimental days, drug-containing solutions were prepared from stock by serial dilution into external solution.