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Rabbit polyclonal anti egfr antibody

Manufactured by Merck Group
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Rabbit polyclonal anti-EGFR antibody is a laboratory reagent used for the detection and quantification of the epidermal growth factor receptor (EGFR) protein in various biological samples.

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Lab products found in correlation

Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Protease inhibitor, by Merck Group (2 mentions) Immobilon p transfer membrane, by Merck Group (2 mentions) Peroxidase conjugated secondary antibody, by Bio-Rad (2 mentions)

Close spelling variants of this product

Anti-EGFR (21 citations) Anti-EGFR antibody (7 citations) Rabbit anti-EGFR (4 citations) EGFR antibody (2 citations) Rabbit anti- (2 citations)

2 protocols using rabbit polyclonal anti egfr antibody

1

EGFR Expression Analysis in U87 Cells

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Total protein extracts were prepared from U87 and U87EGFRvIII cells infected with RRV(AC3-emd or AC3-yCD2) or uninfected, in RIPA buffer containing protease inhibitor (Sigma-Aldrich, St. Louis, MO, USA). Proteins contents were quantified using the Bradford assay. Proteins (20 μg) were resolved in Laemmli buffer by SDS-PAGE and transferred to an Immobilon-P Transfer Membrane (Millipore, Billerica, MA, USA). Western blotting was performed using rabbit polyclonal anti-EGFR antibody (Millipore, Billerica, MA, USA) and rabbit polyclonal anti-beta actin antibody (Abcam, Cambridge, MA, USA). Membranes were treated with peroxidase-conjugated secondary antibodies (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed with SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific, Fremont, CA, USA).
Takahashi M., Valdes G., Hiraoka K., Inagaki A., Kamijima S., Micewicz E., Gruber H.E., Robbins J.M., Jolly D.J., McBride W.H., Iwamoto K.S, & Kasahara N. (2014). Radio-sensitization of gliomas by intracellular generation of 5-fluorouracil potentiates prodrug activator gene therapy with a retroviral replicating vector. Cancer gene therapy, 21(10), 405-410.
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2

EGFR Expression Analysis in U87 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein extracts were prepared from U87 and U87EGFRvIII cells infected with RRV(AC3-emd or AC3-yCD2) or uninfected, in RIPA buffer containing protease inhibitor (Sigma-Aldrich, St. Louis, MO, USA). Proteins contents were quantified using the Bradford assay. Proteins (20 μg) were resolved in Laemmli buffer by SDS-PAGE and transferred to an Immobilon-P Transfer Membrane (Millipore, Billerica, MA, USA). Western blotting was performed using rabbit polyclonal anti-EGFR antibody (Millipore, Billerica, MA, USA) and rabbit polyclonal anti-beta actin antibody (Abcam, Cambridge, MA, USA). Membranes were treated with peroxidase-conjugated secondary antibodies (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed with SuperSignal West Femto Chemiluminescent Substrate (Thermo Scientific, Fremont, CA, USA).
Takahashi M., Valdes G., Hiraoka K., Inagaki A., Kamijima S., Micewicz E., Gruber H.E., Robbins J.M., Jolly D.J., McBride W.H., Iwamoto K.S, & Kasahara N. (2014). Radio-sensitization of gliomas by intracellular generation of 5-fluorouracil potentiates prodrug activator gene therapy with a retroviral replicating vector. Cancer gene therapy, 21(10), 405-410.
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