3,3 Dithiopropionic acid (DTPA), anhydrous methanol, anhydrous ethanol, hydrazine hydrate (HH), hexane, hyaluronic acid sodium salt (HA) from Streptococcus equi, N-3-dimethylaminopropyl-N′-ethylcarbodiimide hydrochloride (EDC), 5,5'-dithiobis-2-nitrobenzoic acid (Ellman's reagent) were purchased from Sigma Aldrich (St. Louis, MO, USA). Dithiothreitol (DTT) was purchased from Gold Biotechnology (St. Louis, MO, USA). Poly(ethylene glycol) divinyl sulfone (PEGDVS) 5 kDa was purchased from JenKem Technology USA (Allen, TX, USA).
Hydrazine hydrate
Manufactured by Merck Group
Sourced in United States, Germany, India, France, China
Hydrazine hydrate is a colorless, crystalline compound. It is the hydrate form of hydrazine, a reducing agent used in various industrial applications. Hydrazine hydrate has a chemical formula of N₂H₄·H₂O and is soluble in water and polar organic solvents.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (25 mentions)
Ethanol, by Merck Group (16 mentions)
Hydrogen peroxide, by Merck Group (11 mentions)
Hydrochloric acid (hcl), by Merck Group (9 mentions)
Methanol, by Merck Group (8 mentions)
Acetonitrile, by Merck Group (7 mentions)
Acetic acid, by Merck Group (7 mentions)
Potassium permanganate, by Merck Group (7 mentions)
Silver nitrate, by Merck Group (6 mentions)
Nitric acid, by Merck Group (6 mentions)
Graphite powder, by Merck Group (6 mentions)
Ethylene glycol, by Merck Group (6 mentions)
Sulfuric acid, by Merck Group (5 mentions)
Sodium nitrate, by Merck Group (5 mentions)
Formic acid, by Thermo Fisher Scientific (5 mentions)
Absolute ethanol, by Merck Group (5 mentions)
Sulphuric acid, by Merck Group (5 mentions)
Potassium hydroxide, by Merck Group (5 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
N n dimethylformamide (dmf), by Merck Group (4 mentions)
144 protocols using hydrazine hydrate
1
Preparation of Thiolated Hyaluronic Acid
2
Synthesis and Characterization of Hydrazones
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Ethyl acetoacetate (EAA), phenylhydrazine,
2,4-dinitrophenylhydrazine (DNP), hydrazine hydrate, p-aminoacetophenone, sodium nitrite, benzaldehyde, 3,5-dimethoxybenzaldehyde,
4-(N,N-dimethylamino)benzaldehyde,
4-hydroxybenzaldehyde, 2-nitrobenzaldehyde, cinnamaldehyde, 4-(N,N-dimethylamino)cinnamaldehyde, sodium
hydroxide, ethyl alcohol, glacial acetic acid, trichloroacetic acid
(TCA), sodium pyrophosphate, reduced glutathione (GSH), and thiobarbituric
acid (TBA) were obtained from Sigma-Aldrich Chemical Co. (St. Louis,
MO, USA). Doxorubicin HCl injection, USP, was purchased from Pfizer
injectables.
2,4-dinitrophenylhydrazine (DNP), hydrazine hydrate, p-aminoacetophenone, sodium nitrite, benzaldehyde, 3,5-dimethoxybenzaldehyde,
4-(N,N-dimethylamino)benzaldehyde,
4-hydroxybenzaldehyde, 2-nitrobenzaldehyde, cinnamaldehyde, 4-(N,N-dimethylamino)cinnamaldehyde, sodium
hydroxide, ethyl alcohol, glacial acetic acid, trichloroacetic acid
(TCA), sodium pyrophosphate, reduced glutathione (GSH), and thiobarbituric
acid (TBA) were obtained from Sigma-Aldrich Chemical Co. (St. Louis,
MO, USA). Doxorubicin HCl injection, USP, was purchased from Pfizer
injectables.
3
Spray-Dried G-Se Hybrid Microballs
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First, Se and GO suspensions were mixed in a volume ratio of 3:1. Second, 10 mL of hydrazine hydrate (Sigma Aldrich), serving as the reducing agent, was slowly added dropwise to the mixture prepared in the first step. Next, the Se/GO/hydrazine hydrate suspension was maintained at 5 °C for inhibiting the reduction of GO to reduced GO (RGO) before spray-drying. Subsequently, a commercial spray dryer (B-290, Buchi) was utilised to form G–Se hybrid microballs from a stable Se/GO/hydrazine hydrate precursor suspension. During spray-drying, the suspension was injected at a feeding rate of 250 mL h−1 and atomised using a spray nozzle, generating aerosol microdroplets. These aerosol microdroplets were sprayed downwards towards a heated zone at 200 °C, which is significantly greater than the boiling point of water. As the aerosol microdroplets passed through the heated zone, the water in the microdroplets evaporated, and the chemical reduction of the nonconductive GO sheets occurred, resulting in conductive RGO sheets. Once the water completely evaporated, the Se particles were encapsulated in the RGO microballs. Subsequently, the final product was washed with ethanol and acetone for removing the residual Triton X-100.
4
Enamel Deproteinization and Calcium Analysis
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Lanthanum nitrate hexahydrate (La(NO3)3·6 H2O, Sigma-Aldrich ChemieGmbH, Steinheim, Germany, ≥99%) was used for the calcium analysis by atomic adsorption spectroscopy. Chloramine T trihydrate solution (Sigma-Aldrich, ≥98%) was used for the storage of extracted teeth as received. Citric acid (Merck, Stettlen, Switzerland, ≥99.5%) was used for the preparation of erosive solutions. Hydrazine hydrate (Sigma-Aldrich, Cat. No. 225819) was used to deproteinate the enamel samples.
5
Synthesis and Characterization of Fluorescent Naphthalimide Derivatives
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4-Bromo-1,8-naphthalic anhydride,
hydrazine hydrate, picolinaldehyde, sodium azide, chloroform, 2,2-dimethylformamide
(DMF), ethanol, all of the anions,l -cysteine, homocysteine,
glutathione and all other chemicals were purchased from Sigma-Aldrich
Pvt. Ltd. (India) and obtained from commercial suppliers. All of the
materials were used directly as purchased. The solvents were dried,
maintaining the conditions of standard procedures. Elix Millipore
water was used throughout the experiments. A Bruker 400 MHz instrument
was employed for detailing 1H and 13C NMR spectra
with the solvents CD3CN, CDCl3, dimethyl sulfoxide
(DMSO)-d6 and D2O. Tetramethylsilane
was used as an internal standard. Chemical shifts are given in δ
ppm units and 1H–1H and 1H–C
coupling constants in Hertz. The following abbreviations are used
to describe spin multiplicities in 1H NMR spectra: s, singlet;
d, doublet; t, triplet; and m, multiplet. A micromass Q-TOF micro
instrument was used to record the mass spectrum using methanol as
the solvent. A PerkinElmer model LS55 spectrophotometer and a Shimadzu
UV-3101PC spectrophotometer were used for recording fluorescence and
UV–vis spectra, respectively. A PerkinElmer 2400 series CHNS/O
analyzer was used for elemental analysis of the compounds.
hydrazine hydrate, picolinaldehyde, sodium azide, chloroform, 2,2-dimethylformamide
(DMF), ethanol, all of the anions,
glutathione and all other chemicals were purchased from Sigma-Aldrich
Pvt. Ltd. (India) and obtained from commercial suppliers. All of the
materials were used directly as purchased. The solvents were dried,
maintaining the conditions of standard procedures. Elix Millipore
water was used throughout the experiments. A Bruker 400 MHz instrument
was employed for detailing 1H and 13C NMR spectra
with the solvents CD3CN, CDCl3, dimethyl sulfoxide
(DMSO)-d6 and D2O. Tetramethylsilane
was used as an internal standard. Chemical shifts are given in δ
ppm units and 1H–1H and 1H–C
coupling constants in Hertz. The following abbreviations are used
to describe spin multiplicities in 1H NMR spectra: s, singlet;
d, doublet; t, triplet; and m, multiplet. A micromass Q-TOF micro
instrument was used to record the mass spectrum using methanol as
the solvent. A PerkinElmer model LS55 spectrophotometer and a Shimadzu
UV-3101PC spectrophotometer were used for recording fluorescence and
UV–vis spectra, respectively. A PerkinElmer 2400 series CHNS/O
analyzer was used for elemental analysis of the compounds.
6
Synthesis of Substituted Aryl Compounds
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Unless otherwise specified, air-sensitive manipulations were carried either in a N2 filled glove box or using standard Schlenk techniques under Ar. 2-Formylphenyl boronic acid, N-iodosuccinimide (AK Scientific); Pd(PPh3)4; Pd2(dba)3, (1,1′-diphenylphosphino)ferrocene (dppf), sodium tert-butoxide (NaOtBu), hydrazine hydrate (Sigma Aldrich), 4-chloro-2-nitronaniline (Combi-Blocks), Na2CO3, zinc, formic acid, and NiCl2·6H2O (Alfa Aesar) were used as purchased.
Organic solvents were dried and distilled using appropriate drying agents prior to use. 1- and 2D NMR spectra were recorded on Bruker Avance 300 MHz or Bruker Avance – III 500 MHz spectrometers. 1H and 13C{1H} NMR spectra were referenced to residual solvent peaks. Elemental analyses were performed at the University of Manitoba using a PerkinElmer 2400 Series II CHNS/O Elemental Analyzer. tBuLH and CF3LH were synthesized according to literature procedures.19 (link)
Organic solvents were dried and distilled using appropriate drying agents prior to use. 1- and 2D NMR spectra were recorded on Bruker Avance 300 MHz or Bruker Avance – III 500 MHz spectrometers. 1H and 13C{1H} NMR spectra were referenced to residual solvent peaks. Elemental analyses were performed at the University of Manitoba using a PerkinElmer 2400 Series II CHNS/O Elemental Analyzer. tBuLH and CF3LH were synthesized according to literature procedures.19 (link)
7
Synthesis of Copper Selenide Nanoparticles
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The raw materials used in this work are sodium
selenide (Na2SeO3, purity ≥99% Sigma-Aldrich),
hydrazine hydrate (N2H4·H2O,
purity ≥80% Sigma-Aldrich), and copper (II) chloride 2-hydrate
(CuCl2·2H2O, purity ≥99% HmBG chemicals).
All chemicals were of analytical grade and used without any purification
or treatment. Deionized water was used as a solvent.
selenide (Na2SeO3, purity ≥99% Sigma-Aldrich),
hydrazine hydrate (N2H4·H2O,
purity ≥80% Sigma-Aldrich), and copper (II) chloride 2-hydrate
(CuCl2·2H2O, purity ≥99% HmBG chemicals).
All chemicals were of analytical grade and used without any purification
or treatment. Deionized water was used as a solvent.
8
Peptide Synthesis via Fmoc SPPS
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Synthesis of peptide precursors was performed manually using fritted syringes on a vacuum manifold via 9‐fluorenylmethyloxycarbonyl (Fmoc) solid‐phase peptide synthesis (SPPS). All reagents were purchased from commercial sources and used without further purification. 2‐Chlorotrityl chloride resin (sub. = 1.2 mmol/g), Wang resin (sub. = 0.73 mmol/g), Oxyma Pure and all standard Fmoc amino acids were sourced from Mimotopes Pty Ltd (Australia). Fmoc‐L‐Dap(Boc)‐OH and Fmoc‐L‐Asp‐OMe were obtained from AK Scientific, Inc. (USA). Fmoc‐Cys(StBu)‐OH was sourced from CreoSalus, Inc. (USA). (1‐Cyano‐2‐ethoxy‐2‐oxoethylidenaminooxy)dimethylamino‐morpholino‐carbenium hexafluorophosphate (COMU), 1‐[bis (dimethylamino)methylene]‐1H‐1,2,3‐triazolo[4,5‐b]pyridinium‐3‐oxide hexafluorophosphate (HATU) and 2,2′‐dipyridyldisulfide (DPDS) were purchased from Combi Blocks (USA). Diisopropylcarbodiimide (DIC), 4‐dimethylaminopyridine (DMAP), diphenyldiselenide, acetoacetone, triisopropylsilane (TIPS), thioanisole, 2,2′‐(ethylenedioxy)diethanethiol (DODT), trifluoroacetic acid (TFA), piperidine, diisopropylethylamine (DIEA), N‐methylmorpholine (NMM), hydrazine hydrate, tris‐carboxyethylphosphine hydrochloride (TCEP.HCl), iodine, dimethylformamide (DMF), dichloromethane (DCM), diethyl ether, acetonitrile and trypsin were purchased from Sigma‐Merck (Australia).
9
Synthesis of Graphene Oxide Nanomaterials
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All materials
used in this research were purchased from Sigma-Aldrich and were of
analytical grade: graphite (fine powder extra pure), sulfuric acid
(H2SO4, 96%), sodium nitrates (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl), zinc sulfate
heptahydrate (ZnSO4·7H2O), ammonium bicarbonate
(NH4HCO3), absolute ethanol (C2H5OH), silver nitrate (AgNO3), copper sulfate (CuSO4), hydrazine hydrate (NH2NH2·H2O, 80%), and methylene blue (MB).
used in this research were purchased from Sigma-Aldrich and were of
analytical grade: graphite (fine powder extra pure), sulfuric acid
(H2SO4, 96%), sodium nitrates (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl), zinc sulfate
heptahydrate (ZnSO4·7H2O), ammonium bicarbonate
(NH4HCO3), absolute ethanol (C2H5OH), silver nitrate (AgNO3), copper sulfate (CuSO4), hydrazine hydrate (NH2NH2·H2O, 80%), and methylene blue (MB).
10
Synthesis of Copper Nanoparticles using Titanium Isopropoxide and Arabic Gum
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Titanium isopropoxide (Sigma-Aldrich, St. Louis, MO, USA, 97%), Arabic gum (Golden Bell, Zapopan, México), copper (II) sulfate pentahydrate (Karal, León, México), potassium iodide (J.T. Baker, Cuautitlán Izcalli, México), acetic acid (J.T. Baker), and hydrazine hydrate (Sigma-Aldrich, St. Louis, MO, USA, 60%) were all analytical grade and were used as received. The microbiological media Sabouraud dextrose agar (BD Bioxon, Cuautitlán Izcalli, México), potato dextrose agar (BD Bioxon, Cuautitlán Izcalli, México), and potato dextrose broth (BD DifcoTM, Cuautitlán Izcalli, México) were used according to the suppliers’ instructions.
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