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Flag f1804 antibody

Manufactured by Merck Group
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The FLAG (#F1804) antibody is a product offered by Merck Group. It is a monoclonal antibody that recognizes the FLAG epitope, a peptide sequence commonly used as a protein tag for recombinant protein expression and purification. The antibody can be used to detect and purify proteins that have been engineered to contain the FLAG tag.

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Lab products found in correlation

Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Nitrocellulose membrane, by GE Healthcare (2 mentions) Ecl detection reagent, by Cytiva (2 mentions) P 4e bp1 t37 46, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions) P 4ebp1 s65, by Cell Signaling Technology (2 mentions) P 4ebp1 t70, by Cell Signaling Technology (2 mentions) Gst antibody sc 138, by Santa Cruz Biotechnology (2 mentions) Raptor, by Cell Signaling Technology (2 mentions) Ecl kit, by Bio-Rad (1 mentions) Gapdh antibody sc 32233, by Santa Cruz Biotechnology (1 mentions) Ha antibody sc 805, by Santa Cruz Biotechnology (1 mentions) Strep hrp antibody 71591, by Merck Group (1 mentions) Ha antibody mms 101p, by Fortrea (1 mentions)

Close spelling variants of this product

F1804 (465 citations) Flag antibody (199 citations) Anti-FLAG antibody (F1804) (48 citations) FLAG (F1804) (21 citations) Anti-FLAG M2 antibody (F1804; (15 citations) Monoclonal anti-Flag M2 antibody (F1804), (6 citations) Mouse monoclonal anti-FLAG M2 antibody (F1804) (4 citations) Mouse anti-FLAG antibody (F1804, (4 citations) Antibody against FLAG (F1804) (3 citations) FLAG® M2 monoclonal antibody (F1804, (3 citations)

4 protocols using flag f1804 antibody

1

Immunoblotting Analysis of Protein Phosphorylation

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Cells were washed with PBS once, disrupted on ice for thirty minutes in NP-40 (50 mM Tris [pH 7.4], 1% NP-40, 150 mmol/L NaCl, 40 mmol/L NaF) or RIPA lysis buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitors (Pierce Chemical) and cleared by centrifugation. Protein concentration was determined with BCA reagent from Pierce. Equal amounts of protein (10 to 50 µg) in cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes (GE healthcare), immunoblotted with specific primary and secondary antibodies and detected by chemiluminescence with the ECL detection reagents from Amersham Biosciences. Antibodies for P-AKT (S473) (#4060L), P-p70S6K (T389) (#9234L), P-S6 (S240/244) (#5364L) and P-S6 (S235/236) (#4858L), P-4EBP1 (T37/46) (#9459L), P-4EBP1 (S65) (#9451L), P-4EBP1 (T70) (#9455L), β-actin (#4970S), mTOR (#2972S) and Raptor (#2280S) were purchased from Cell Signaling Technology. The FLAG (#F1804) antibody was purchased from Sigma. The GST antibody (#sc-138) was from Santa Cruz.
Rodrik-Outmezguine V.S., Okaniwa M., Yao Z., Novotny C.J., McWhirter C., Banaji A., Won H., Wong W., Berger M., de Stanchina E., Barratt D.G., Cosulich S., Klinowska T., Rosen N, & Shokat K.M. (2016). Overcoming mTOR Resistance Mutations with a New Generation mTOR Inhibitor. Nature, 534(7606), 272-276.
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2

Immunoblotting Analysis of Protein Phosphorylation

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Cells were washed with PBS once, disrupted on ice for thirty minutes in NP-40 (50 mM Tris [pH 7.4], 1% NP-40, 150 mmol/L NaCl, 40 mmol/L NaF) or RIPA lysis buffer (Thermo Scientific) supplemented with protease and phosphatase inhibitors (Pierce Chemical) and cleared by centrifugation. Protein concentration was determined with BCA reagent from Pierce. Equal amounts of protein (10 to 50 µg) in cell lysates were separated by SDS-PAGE, transferred to nitrocellulose membranes (GE healthcare), immunoblotted with specific primary and secondary antibodies and detected by chemiluminescence with the ECL detection reagents from Amersham Biosciences. Antibodies for P-AKT (S473) (#4060L), P-p70S6K (T389) (#9234L), P-S6 (S240/244) (#5364L) and P-S6 (S235/236) (#4858L), P-4EBP1 (T37/46) (#9459L), P-4EBP1 (S65) (#9451L), P-4EBP1 (T70) (#9455L), β-actin (#4970S), mTOR (#2972S) and Raptor (#2280S) were purchased from Cell Signaling Technology. The FLAG (#F1804) antibody was purchased from Sigma. The GST antibody (#sc-138) was from Santa Cruz.
Rodrik-Outmezguine V.S., Okaniwa M., Yao Z., Novotny C.J., McWhirter C., Banaji A., Won H., Wong W., Berger M., de Stanchina E., Barratt D.G., Cosulich S., Klinowska T., Rosen N, & Shokat K.M. (2016). Overcoming mTOR Resistance Mutations with a New Generation mTOR Inhibitor. Nature, 534(7606), 272-276.
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3

Western Blot Analysis of Protein Lysates

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Cells were lysed in buffer containing 50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1% (w/v) CHAPS and Sigma protease inhibitor cocktail, and the total cell lysates were resolved with SDS-PAGE gels. The following antibodies were used: GFP antibody (632381) from Clontech; GAPDH antibody (sc-32233) from Santa Cruz; Flag antibody (F1804) from Sigma; HA antibody (SC-805) from Santa Cruz. HRP-linked secondary antibodies were used and blots visualized with the ECL kit (Bio-Rad).
Yang Y., Fan X., Mao M., Song X., Wu P., Zhang Y., Jin Y., Yang Y., Chen L.L., Wang Y., Wong C.C., Xiao X, & Wang Z. (2017). Extensive translation of circular RNAs driven by N6-methyladenosine. Cell Research, 27(5), 626-641.
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4

Antibody Sourcing for Protein Detection

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The HA antibody (MMS-101P) was purchased from Covance. The PJA2 antibody (A302-991A) was purchased from Bethyl. The CCNT1 (sc-8127), CDK9 (sc-8338), and Tat (sc-65913) antibodies were purchased from Santa Cruz Biotechnology. The STREP-HRP antibody (71591) was purchased from Millipore. The FLAG antibody (F1804) was purchased from Sigma. The GAPDH antibody (AM4300) was purchased from Ambion.
Faust T.B., Li Y., Jang G.M., Johnson J.R., Yang S., Weiss A., Krogan N.J, & Frankel A.D. (2017). PJA2 ubiquitinates the HIV-1 Tat protein with atypical chain linkages to activate viral transcription. Scientific Reports, 7, 45394.
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