Trisodium citrate dehydrate

Gold(iii)chloride hydrate (HAuCl₄·4H₂O) 99% and trisodium citrate dehydrate (Na₃C₆H₅O₇·2H₂O) 99% were purchased from Sigma-Aldrich (Oakville, ON, Canada). All the chemicals were of analytical grade and used as received without further purification. Milli-Q water (resistance > 18.2 MΩ) was used for solution preparation and reaction was RNase-free. Human 8-oxoguanine DNA glycosylase (hOGG1), human alkyladenine DNA glycosylase (hAAG), human apurinic/apyrimidinic endonuclease 1 (APE1), and CutSmart (50 mM KAc, 20 mM Tris-Ac, 10 mM Mg(Ac)₂, 100 μg mL^–1 BSA, pH 7.9) were purchased from New England Biolabs Inc. (Beverly, MA, U.S.A.). HPLC-purified oligonucleotides (Table S1†) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) and Takara Biotechnology Co. Ltd. (Dalian, China).

Polyacrylamide (PAM) with a molecular weight of 5–6 megadaltons (MDa) was purchased from International Laboratory USA (South San Francisco, USA). Iron(iii) chloride hexahydrate (FeCl₃·6H₂O) was obtained from Acros (New Jersey, USA). Trisodium citrate dehydrate, urea, ethylenediamine, (3-mercaptopropyl)trimethoxysilane (MPS), urea, sodium diethyldithiocarbamate (DDTC, ≥99%) and matrix α-cyano-4-hydroxycinnamic acid (CHCA) were ordered from Sigma Aldrich (St. Louis, USA). Cisplatin (≥98%), glutathione (GSH), thioctic acid, carbonyldiimidazole, 1H-pyrazole-1-carboxamidine hydrochloride, tris(2-carboxyethyl)phosphine hydrochloride and triethanolamine were bought from Meryer (Shanghai, China).
For solvents, ethanol (EtOH), acetonitrile (MeCN), methanol (MeOH), and trifluoroacetic acid (TFA, 99%) were provided by VWR International Company (Radnor, Pennsylvania), while acetone (AC), dichloromethane (DCM), dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) were from RCI Labscan Ltd. (Bangkok, Thailand). All chemicals were of high performance liquid chromatography (HPLC) or analytical grade and utilized with no further purification, and distilled water (18.2 MΩ cm) was purified by using a Milli-Q ICW3000 system (Millipore, Billerica, MA) and degassed with nitrogen prior to usage.

Gold chloride trihydrate (HAuCl₄⋅3H₂O) and trisodium citrate dehydrate (Na₃C₆H₅O₇⋅2H₂O) were purchased from Sigma-Aldrich (St Louis, MO, USA). Milli-Q water with a resistance of 18.2 M Ω was used in all experiments. All secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Fully cytosine-phosphate-guanine-oligodeoxynucleotide (CpG) 1826 with a sequence of 5′-TCCATGACGTTCCTGACGTT-3′ were synthesized and purified in Takara Company (Daliang, People’s Republic of China). PCV2 strain was stored in our lab. Porcine Kidney-15 cells, DC2.4 dendritic cells, and RAW264.7 macrophages were obtained from ATCC (Manassas, VA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), RPMI-1640, penicillin, and streptomycin were purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS), were purchased from GIBCO (Carlsbad, CA, USA). Carbopol^® 971P NF polymer was purchased from Lubrizol (Cleveland, OH, USA). Freund’s adjuvant was purchased from Sigma-Aldrich. The oligopeptide 169-STIDYFQPNNKR was synthesized by GL Biochem (Shanghai, People’s Republic of China), a cysteine was added to the N-terminal end of the oligopep-tide, and then conjugated to bovine serum albumin (BSA) using 4-(N-maleimidomethyl) cyclohexanecarboxylic acid N-hydroxysuccinimide ester according to the manufacturer’s instruction (Sigma-Aldrich).