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N succinimidyl 3 2 pyridyldithio propionate spdp

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N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) is a heterobifunctional crosslinking reagent that contains an N-hydroxysuccinimide (NHS) ester and a pyridyldisulfide group. The NHS ester group reacts with primary amines, while the pyridyldisulfide group reacts with sulfhydryl groups, enabling the conjugation of two different molecules.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Polyvinyl alcohol mw 2000 pvoh, by Thermo Fisher Scientific (1 mentions) Dna grade nap 10 columns, by GE Healthcare (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dnase, by Merck Group (1 mentions) Ab1206, by Abcam (1 mentions) Ab81051, by Abcam (1 mentions) Mouse iggk bp hrp, by Santa Cruz Biotechnology (1 mentions) Imfinzi, by AstraZeneca (1 mentions) Human pd l1 simplestep elisa kit, by Abcam (1 mentions) Mirna mimic, by Thermo Fisher Scientific (1 mentions) Mirna mimic, by Qiagen (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Cetylpyridinium chloride cpc, by Merck Group (1 mentions)

Close spelling variants of this product

Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (2 citations) Propionate (2 citations)

4 protocols using n succinimidyl 3 2 pyridyldithio propionate spdp

1

Multifunctional Immunostimulatory Hydrogel Nanoparticles

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Poly(ethylene glycol) diacrylate (Mn 700) (PEG700DA), 2-aminoethyl methacrylate hydrochloride (AEM), diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO), thiol modified CpG 1826 (C6-S-S-C6-tccatgacgttcctgacgtt), dithiothreitol (DTT), sucrose, and DNase, RNase free sterile water were purchased from Sigma-Aldrich. Tetraethylene glycol monoacrylate (HP4A) was synthesized in house. Cysteine modified OVA257–264 (CSIINFEKL) were purchased from Peptide 2.0. Trifluoroacetic acid, methanol, dimethyl sulfoxide (DMSO), PTFE (polytetrafluoroethylene) syringe filters (13 mm membrane, 0.22-μm pore size), HPLC grade water, and acetonitrile were obtained from Fisher Scientific. Conventional filters (2 μm) were purchased from Agilent Technologies, and poly(vinyl alcohol) (Mw 2000) (PVOH) was purchased from Acros Organics. N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) was purchased from Thermo Scientific. Ortho-pyridyldisulfide-PEG-N-hydroxylsuccinimide ester (NHS-PEG (2k)-OPSS) was purchased from Creative PEGworks. PRINT molds (80 nm × 320 nm) were obtained from Liquidia Technologies. DNA grade NAP-10 columns were purchased from GE Healthcare. RPMI1640 medium, penicillin and streptomycin, L-glutamine, and fetal bovine serum (FBS) were all from Life Technologies.
Kapadia C.H., Tian S., Perry J.L., Luft J.C, & DeSimone J.M. (2016). Reduction Sensitive PEG Hydrogels for Codelivery of Antigen and Adjuvant To Induce Potent CTLs. Molecular pharmaceutics, 13(10), 3381-3394.
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2

Durvalumab Binding Assay Protocol

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Durvalumab (Imfinzi; AstraZeneca) was obtained from Universal Oncology Center. The FITC‐anti‐human PD‐L1 (ab224074; Abcam), goat polyclonal Ab to human IgG (FITC) (ab81051; Abcam), Rb polyclonal Ab to 6× His‐tag (FITC) (ab1206; Abcam), mouse‐IgGK BP‐HRP (sc‐516102; Santa Cruz Biotechnology). N‐succinimidyl 3‐(2‐pyridyldithio)propionate (SPDP, 21857; Thermo Fisher Scientific). Human PD‐L1 SimpleStep ELISA Kit (ab214565; Abcam).
Zhang C., Xiong J., Lan Y., Wu J., Wang C., Huang Z., Lin J, & Xie J. (2020). Novel cucurmosin‐based immunotoxin targeting programmed cell death 1‐ligand 1 with high potency against human tumor in vitro and in vivo. Cancer Science, 111(9), 3184-3194.
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3

Synthesis of Multifunctional Nanodrugs

Cited 4 times
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The nanodrugs consisted of magnetic nanoparticles (MN, magnetic resonance imaging moiety) conjugated to LNA ASO or miRNA mimics (Exiqon, Denmark) as a therapeutic moiety (Fig. 1). The sequences of miRNA mimics, inhibitors and scramble controls used for the synthesis of the nanodrugs are included in Supplemental Table 1. Nanoparticles with a size of ~30 nm were used for conjugation to the oligonucleotides28 . Synthesis of the prototype nanodrugs have been previously described by us6 (link),29 (link) and involved: (1) synthesis of dextran-coated magnetic nanoparticles30 (link); (2) conjugation of Cy5.5 fluorescent dye. The number of dyes per magnetic nanoparticle was determined as 3.2 (6); (3) conjugation of antisense LNA inhibiting oligonucleotides (ASO) or miRNA mimics through heterobifunctional linker N-succinimidyl 3-[2-pyridyldithio]-propionate (SPDP; Thermo Scientific Co., Rockford, IL) to produce MN-ASO or MN-miRNA31 (link)–33 (link). ASO-scrambled (MN-ASOscr, single stranded) and mimics-scrambled (MN-miRNAscr, double stranded) nanodrugs were also synthesized as controls. The number of oligos per MN was determined as 4.0 using the electrophoresis analysis method described previously33 (link).
Wang P., Liu Q., Zhao H., Bishop J.O., Zhou G., Olson L.K, & Moore A. (2020). miR-216a-targeting theranostic nanoparticles promote proliferation of insulin-secreting cells in type 1 diabetes animal model. Scientific Reports, 10, 5302.
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4

Chitosan-PEI Complexation for Gene Delivery

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Chitosan (MW = 10 kDa) with a 92% degree of deacetylation was supplied by AK Biotech Ltd. (Jinan, Shandong, China). Polyethylenimine (PEI,MW = 1.8 and 25 kDa) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Shanghai, China). N-Succinimidyl 3-(2-pyridyldithio)-propionate (SPDP) was provided by Thermo Scientific (Rockford, LA, USA). PEI 25 kDa was purified by dialysis in deionized water and lyophilization before used as positive control for gene delivery. Alizarin Red S, cetylpyridinium chloride (CPC), and alkaline phosphatase (ALP) yellow liquid substrate were obtained from Sigma. The BCA™ protein assay kit was obtained from Thermo.
Yue J., Wu J., Liu D., Zhao X, & Lu W.W. (2015). BMP2 gene delivery to bone mesenchymal stem cell by chitosan-g-PEI nonviral vector. Nanoscale Research Letters, 10, 203.
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