Immunostaining reactivity was observed using light microscopy (Olympus BX-53 with CCD DP74, Shinjuku, Japan). The results were analyzed by two pathologists (Xi Y and Chen H) who acted independently and were blind to the clinicopathological characteristics of the study. The analyses of the two pathologists were compared, and any discrepancies were reassessed to arrive at a consensus. The expressions of GOLM1 and FAM49B in tumor and immune cells were recorded for further research.
Ccd dp74
Manufactured by Olympus
Sourced in Japan
The CCD DP74 is a digital camera module designed for use in microscopy and imaging applications. It features a high-resolution CCD (Charge-Coupled Device) sensor that captures detailed images. The CCD DP74 provides reliable image capture capabilities for various scientific and research purposes.
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Lab products found in correlation
3 protocols using ccd dp74
1
Immunostaining Analysis of GOLM1 and FAM49B
2
Blinded Pathologist Immunostaining Scoring
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Immunostaining reactivity was observed using light microscopy (Olympus BX-53 with CCD DP74). Results were scored by two pathologists (Guo W and Chen H) who were independent and blinded to the clinicopathological characteristics of the study. The scores of the two pathologists were compared, and any discrepancies were reassessed to achieve a consensus.
3
Characterization of Nano-Sized Bubbles
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The morphology of NBAV was observed by scanning electron microscopy (SEM, S-4800, HITACHI, Japan) and the images were captured by software (HITACHI S-4800, PC-SEM). During the NBAV preparation, the lipid dye DiI (5 μM) was dissolved in the lipid solution for better observation. All other procedures remained the same. The obtained NBAV were examined under a fluorescence microscope (Olympus CKX53, Japan), and images were captured (Camera: Olympus CCD DP74).
The characterization of NBAV, such as size distribution, zeta potential, polydispersity index and stability of NBAV, were measured as described below. The diluted NBAV (4.5 ± 1.0×108 bubbles/mL) solutions were kept at 4 ℃, and then the size distribution was analyzed after 0, 12, 24, 36, 48, 60 and 72 h, respectively, by a NanoPlus-3 zeta/nano particle analyzer (Micromeritics Instrument CORP, USA). Furthermore, 1mL of NBAV stock solution was diluted with a 9 mL mixture of PBS (0.01 M) and fetal bovine serum (10%, FBS, HyClone, USA), and then size was measured after 0, 20, 40, 60, 80, 100 and 120 min at 37 ℃. The zeta potential of NBAV was also measured with this analyzer. All the experiments were repeated three times.
The characterization of NBAV, such as size distribution, zeta potential, polydispersity index and stability of NBAV, were measured as described below. The diluted NBAV (4.5 ± 1.0×108 bubbles/mL) solutions were kept at 4 ℃, and then the size distribution was analyzed after 0, 12, 24, 36, 48, 60 and 72 h, respectively, by a NanoPlus-3 zeta/nano particle analyzer (Micromeritics Instrument CORP, USA). Furthermore, 1mL of NBAV stock solution was diluted with a 9 mL mixture of PBS (0.01 M) and fetal bovine serum (10%, FBS, HyClone, USA), and then size was measured after 0, 20, 40, 60, 80, 100 and 120 min at 37 ℃. The zeta potential of NBAV was also measured with this analyzer. All the experiments were repeated three times.
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