Immulite 1000 analyzer

Manufactured by Siemens

Sourced in Germany, United States, United Kingdom

The Immulite 1000 Analyzer is an automated immunoassay system used for the detection and measurement of various analytes in clinical laboratory settings. It is designed to perform a range of immunoassay tests, including those for hormones, proteins, and other biomolecules. The Immulite 1000 Analyzer is capable of processing multiple samples simultaneously and provides accurate and reliable results.

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Spelling variants of this product

Immulite 1000 (226 citations) Immulite analyzer (23 citations) DPC IMMULITE 1000 analyzer (6 citations) IMMULITE 1000 Immunoassay analyzer (3 citations) Immulite 1000 auto analyzer (2 citations)

35 protocols using immulite 1000 analyzer

Measuring Serum IGF-I and GH Levels

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Serum IGF-I levels were analyzed using a solid-phase enzyme labeled chemiluminescent immunometric assay with an IMMULITE 1000 analyzer (Siemens Healthcare Diagnostics Products Ltd., Llanberis, Gwynedd, UK) with an intra-assay coefficient of variation (CV) of 3.1%–4.3%. The IGF-I international reference preparation was 87/518. GH serum levels were determined using a solid-phase two-site immunometric chemiluminescent assay with an IMMULITE 1000 analyzer (Siemens Healthcare Diagnostics Products Ltd., Llanberis, Gwynedd, UK) with inter- and intra-assay CVs of 5.5%–6.2% and 5.3%–6.5%, respectively. The international reference preparation for GH was 98/574.
The IGF-I and GH levels obtained in the last measurement were considered for the definition of disease control. The controlled disease cases were defined as those in which the patient presented a baseline GH level < 1 mg/L and an IGF-I level within the normal range for their age and sex [17 (link)].

GuimarãesSá A.M., Ferreira P.A., Souza M.T., Nascimento G.C., da Silva Pereira Damianse S., de Carvalho Rocha V.C., dos Santos Faria M, & de Souza Paiva Ferreira A. (2018). Higher Income and Integration into the Workforce Are the Main Factors Associated with Quality of Life in Acromegalic Patients in Northeastern Brazil. International Journal of Endocrinology, 2018, 6135080.

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Cytokine Analysis Using Immulite 1000

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High-sensitivity TNF, IL-1b, IL-6 and IL-10 were analyzed by immunometric assays on an Immulite 1000 Analyzer (Siemens Healthcare Diagnostics, Los Angeles, CA, USA) according to the instructions of the manufacturer.

Hilderman M., Qureshi A.R., Al-Abed Y., Abtahi F., Lindecrantz K., Anderstam B, & Bruchfeld A. (2015). Cholinergic anti-inflammatory pathway activity in dialysis patients: a role for neuroimmunomodulation?. Clinical Kidney Journal, 8(5), 599-605.

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Cytokine Profiling in Serum

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A chemiluminescent immunometric assay was used to detect serum interleukin-2 receptor (IL-2R), IL-1β, IL-10, IL-8, and tumor necrosis factor-α (TNF-α) (IMMULITE 1000 Analyzer, Siemens). Serum IL-6 was measured using the electrochemiluminescence method (Roche Diagnostics, South San Francisco, CA, USA) [23 (link)].

Gong Z., Gao R., Ba L., Liu Y., Hou H, & Zhang M. (2023). The Peripheral Immune Traits Changed in Patients with Multiple System Atrophy. Brain Sciences, 13(2), 205.

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Comprehensive Metabolic Profiling in Fasting Blood

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Fasting venous blood samples were collected for measurement of blood hemoglobin, serum urea, creatinine, calcium, phosphate, intact parathyroid hormone, high-sensitivity C-reactive protein (hs-CRP), albumin, cholesterol, triglyceride, fetuin-A, N-terminal pro-brain natriuretic peptide (NT-pro-BNP), and cardiac troponin. Intact parathyroid hormone was measured by Immulite 1000 Analyzer (Siemens Healthcare Diagnostics, Deerfield, IL). hs-CRP was measured using the Tina-quant C-reactive protein latex ultra-sensitive assay. Albumin was measured using the bromcresol purple method, and total cholesterol and triglyceride were measured by the Hitachi 911 analyzer (Roche Diagnostics GmbH, Mannheim, Germany). High-density lipoprotein-cholesterol was measured by the precipitation of Apo B containing lipoproteins with phosphotungstate, and low-density lipoprotein-cholesterol was calculated using the Friedewald formula. Serum fetuin-A was determined using a human fetuin-A enzyme-linked immunosorbent assay kit (Epitope Diagnostics, San Diego, CA). NT-pro-BNP and cardiac troponin T were quantified by electrochemiluminescence immunoassay on the Elecsys 2010 analyzer (Roche Diagnostics, Indianapolis, IN).

Wang A.Y., Sea M.M., Ng K., Wang M., Chan I.H., Lam C.W., Sanderson J.E, & Woo J. (2019). Dietary Fiber Intake, Myocardial Injury, and Major Adverse Cardiovascular Events Among End-Stage Kidney Disease Patients: A Prospective Cohort Study. Kidney International Reports, 4(6), 814-823.

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Thyroid Function Assessment Protocol

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Serum and urine samples were immediately stored at −20°C for subsequent analysis. Serum TSH, FT4, TT4, TT3, and anti-TPO were estimated by the Chemiluminescence technique using commercially available kits from Siemens Diagnostics (Germany) with Immulite-1000 analyzer. The analytical sensitivity and total precision values for TSH, FT4, TT4, and TT3 assays were 0.01 μIU/ml and 2.2%, 0.35 ng/dl and 2.7%, 0.4 μg/dl and 2.5%, and 35ng/dl and 2.2%, respectively. The laboratory reference ranges were TSH (0.4–4 μIU/ml), FT4 (0.8–1.9 ng/dl), TT4 (4.5–12 μg/dl), TT3 (81–178 ng/dl), and the interassay coefficients of variation (CV) for the assays were 8.9%, 5.5%, 6.7%, and 9.3%, respectively. The corresponding values for interassay CV, total precision, and analytical sensitivity for anti-TPO were 10.5%, 7.6%, and 7 IU/ml. Anti-TPO Ab was considered elevated if levels were >35 IU/ml. Urinary iodine concentration (UIC) was determined in all participants by the ammonium persulfate method based on the Sandell–Kolthoff reaction. The interassay CV for UIC was 4%.[20 (link)] The UIC <150 μg/L in pregnant women was taken as evidence of insufficient iodine intake.[21 ]

Pramanik S., Mukhopadhyay P., Bhattacharjee K., Bhattacharjee R., Mukherjee B., Mondal S.A., Bandhopadhay S., Biswas S., Chowdhury S, & Ghosh S. (2020). Trimester-Specific Reference Intervals for Thyroid Function Parameters in Indian Pregnant Women during Final Phase of Transition to Iodine Sufficiency. Indian Journal of Endocrinology and Metabolism, 24(2), 160-164.

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Comprehensive Biomarker Analysis of Fasting Plasma

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Blood samples were obtained in a fasting state in the morning the day before the surgical procedure, and stored in -80°C. Interleukin (IL)-6, IL-8, TNF, IGF-1 and testosterone were analysed by immunometric assays on an Immulite 1000 Analyzer (Siemens Healthcare Diagnostics, Los Angeles, CA, USA) according to the instructions of the manufacturer. Plasma pentosidine was analysed by reverse-phase high performance liquid chromatography (HPLC). The following biomarkers were analysed with ELISA technique; 8-OHdG (Japan Institute for the Control of Aging, Shizuoka, Japan), osteoprotegerin (OGP) (Quidel Corporation, San Diego, CA, USA), human soluble α-Klotho (Immuno-Biological Laboratories Co., Ltd, Fujioka-shi, Japan), C-terminal FGF-23 (Immutopics Inc, San Clemente, CA, USA), Human N-MID Osteocalcin ELISA (IDS Nordic a/s, Herlev, Denmark), GLA-OC) and GLU-OC (Takara Bio Inc, Kusatsu, Japan). N-MID OC ELISA measuring total OC is more stable and reproducible than assays measuring intact OC only. Analyses of hsCRP, intact parathyroid hormone (iPTH), plasma cholesterol, triglycerides, HDL-cholesterol, creatinine, albumin, calcium, magnesium, phosphate, alkaline phosphatase (ALP), 25(OH) and 1,25(OH) D-vitamin were performed with validated routine methods at the accredited Clinical Chemical Laboratory Lab at the Karolinska University Hospital, Stockholm, Sweden.

Stenvinkel P., Luttropp K., McGuinness D., Witasp A., Rashid Qureshi A., Wernerson A., Nordfors L., Schalling M., Ripsweden J., Wennberg L., Söderberg M., Bárány P., Olauson H, & Shiels P.G. (2017). CDKN2A/p16INK4a expression is associated with vascular progeria in chronic kidney disease. Aging (Albany NY), 9(2), 494-505.

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SARS-CoV-2 Diagnostic and Cytokine Profiling

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Routine laboratory tests. The blood routine, liver and kidney function, heart function, coagulation function and infection markers were performed by automated analyzers according to the manufacturers' instructions.
Real time RT-PCR. The clinical samples obtained from patients at admission or during the hospital stay were maintained in viral-transport medium. SARS-CoV-2 was confirmed by using TaqMan One-Step RT-PCR Kits from Shanghai Huirui Biotechnology Co.,Ltd and Shanghai BioGerm Medical Biotechnology Co.,Ltd. Briefly, RNA was extracted from clinical samples. 5 μL of RNA was used for real-time RT-PCR, which targeted the ORF1ab and N gene. Real-time RT-PCR was performed using the following conditions: 50 °C for 15 min and 95 °C for 5 min, 45 cycles of amplification at 95 °C for 10 s and 55 °C for 45 s. The positive SARS-CoV-2 real time RT-PCR result was defined if both ORF1ab and N cycle thresholds were <35.
Cytokine profile analysis. Serum samples were collected from study participants. The levels of IL-1β, IL-2R, IL-8, IL-10, and TNF-α in serum were measured according to an automatic procedure of a solid-phase two-site chemiluminescent immunometric assay via IMMULITE 1000 Analyzer (Siemens). The level of IL-6 was measured by electrochemiluminescence method (Roche Diagnostics).

Wang F., Hou H., Wang T., Luo Y., Tang G., Wu S., Zhou H, & Sun Z. (2020). Establishing a model for predicting the outcome of COVID-19 based on combination of laboratory tests. Travel Medicine and Infectious Disease, 36, 101782.

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Biomarker Analysis in Kidney Disease

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Blood samples were collected from patients after they had fasted overnight. They were
collected from hemodialysis patients in the midweek and from patients with stages 3
to 5 chronic kidney disease undergoing peritoneal dialysis at regular clinic visits.
Plasma and serum were stored at −70°C pending biochemical analysis. Serum
concentrations of parathyroid hormone, interleukin-6, and high-sensitivity C-reactive
protein (hsCRP) were quantified using immunometric assays on an Immulite automatic
analyzer (Siemens Medical Solutions Diagnostics, USA). Other circulating risk markers
were measured by using a commercial ELISA kit (R&D Systems, Inc. USA): serum OPG
(R&D Systems Inc.) and plasma fibroblast growth factor-23 (FGF-23) (Millipore
Corporate Headquarters, USA). The serum level of cardiac troponin I level was
analyzed by an immunometric assay, using an Immulite 1000 Analyzer (Siemens Medical
Solutions Diagnostics) according to the manufacturer's instructions. Serum albumin,
creatinine, uric acid, urea, potassium, calcium, phosphate, and total and
high-density lipoprotein cholesterol concentrations were determined using a Konelab
20XT centrifuge analyzer (Thermo Electron Corporation, Finland). All analyses were
performed at the Renal Medicine Laboratory, Clinical Research Center, Karolinska
Institute, Stockholm, Sweden.

Nascimento M.M., Hayashi S.Y., Riella M.C, & Lindholm B. (2014). Elevated levels of plasma osteoprotegerin are associated with all-cause mortality risk and atherosclerosis in patients with stages 3 to 5 chronic kidney disease. Brazilian Journal of Medical and Biological Research, 47(11), 995-1002.

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Progesterone Quantification in Bovine Serum

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Blood was collected by venipuncture of the coccygeal vein into 10 mL dry vacutainers (Becton-Dickinson), allowed to clot and centrifuged (2000 g for 15 min) within 30 min of collection. Serum samples were transferred to the laboratory at 4 °C and then stored at − 20 °C until analysis. Progesterone concentrations were measured by a chemiluminescent immunoassay in an IMMULITE 1000 analyzer (Siemens Healthcare Diagnostics) using a commercial kit (IMMULITE 1000 Progesterone Kit, Siemens Healthcare Diagnostics). The assay’s sensitivity was 0.2 ng/mL, and the inter-assay coefficient of variation was < 10%. The cut-off value used to define cows with high P4 concentrations was 1 ng/mL. Means and range of values for the groups of cow with high and low P4 concentrations are shown in Table 1.

Pereira G., Guo Y., Silva E., Bevilacqua C., Charpigny G., Lopes-da-Costa L, & Humblot P. (2022). Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types. BMC Genomics, 23, 82.

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Serum Cytokine Profiling Assay

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Serum samples were collected from all participants upon admission. The levels of IL-1β, IL-2 R, IL-8, IL-10, and TNF-α were determined using an automated solid-phase two-site chemiluminescent immunometric assay on the IMMULITE 1000 Analyzer (Siemens). The level of IL-6 was measured using the electrochemiluminescence method with Roche Diagnostics. These assays were performed according to the manufacturer’s instructions to ensure accurate and reliable measurements of cytokine levels in the serum samples.

Wang Y., Yuan X., Wang T., Wei W., Wu S, & Hou H. (2024). Comprehensive evaluation of immune dysregulation in secondary hemophagocytic lymphohistiocytosis. Virulence, 15(1), 2342276.

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