Ovalbumin (OVA), lysozyme (Lyz), acrylamide (AM), N,N-methylene-bis-acrylamide (MBAM), ammonium persulfate (APS), N,N,N’,N’-tertramethylethylenediamine (TEMED), 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES), phosphate-buffered saline (PBS), 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid (iniferter agent), paraformaldehyde, Dulbecco’s modified Eagle’s medium with nutriment mixture F12 (DMEM/F12), fetal bovine serum (FBS), and PenStrep were provided by Sigma-Aldrich (Molsheim, France). Iron (II) chloride tetrahydrate (FeCl2·4H2O), iron (III) chloride hexahydrate (FeCl3·6H2O), iron (III) nitrate nonahydrate (Fe(NO3)3·9H2O), acetone, diethyl ether, ethanol at 96%, chloroform and methanol were provided by VWR Chemicals (Lutterworth, UK). Ammonia solution (NH3; 20%) and acetic acid were provided by Carlo Erba (Val-de-Reuil, France). Hydrochloric acid was provided by Merck (Molsheim, France). Acryloxyethylthiocarbamoyl-Rhodamine B was purchased from Polysciences, Inc. (Nanterre, France) The green fluorescent protein (GFP) was kindly provided to us by the Dahan group of the Curie Research Institute (Paris, France). All materials were used as received without any purification.
Iron 3 chloride hexahydrate
Manufactured by Avantor
Sourced in France
Iron (III) chloride hexahydrate is a chemical compound with the formula FeCl3·6H2O. It is a crystalline solid that is soluble in water and other polar solvents. Iron (III) chloride hexahydrate is commonly used as a coagulant in water treatment and as a reagent in various chemical synthesis processes.
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Lab products found in correlation
Iron 2 chloride tetrahydrate, by Avantor (3 mentions)
Iron 3 nitrate nonahydrate, by Avantor (2 mentions)
Iron 2 chloride tetrahydrate, by Merck Group (2 mentions)
Nitric acid, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Glutaraldehyde solution, by Merck Group (2 mentions)
Ethanol, by Avantor (2 mentions)
Polyvinylpyrrolidone, by Merck Group (2 mentions)
Diethylene glycol, by Merck Group (2 mentions)
Hydrazine hydrate, by Merck Group (2 mentions)
N methyldiethanolamine, by Merck Group (2 mentions)
Ammonium sulfide solution, by Merck Group (2 mentions)
Sodium cacodylate trihydrate, by Merck Group (2 mentions)
Copper 2 nitrate hemi pentahydrate, by Merck Group (2 mentions)
Pen strep, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Pentanoic acid, by Merck Group (1 mentions)
8 protocols using iron 3 chloride hexahydrate
1
Synthesis of Fluorescent Polymer Nanoparticles
2
Synthesis of Dextran-Coated Iron Oxide Nanoparticles
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UL-D nanoparticles were synthesized by classical co-precipitation of iron oxide in an alkaline solution in the presence of dextran, a high molecular weight polymer of d-glucose.20 ,21 (link) Six mmol iron (II) chloride tetrahydrate (FeCl2·4H2O) and 27.8 mmol iron (III) chloride hexahydrate (FeCl3·6H2O) (VWR International GmbH, Darmstadt, Germany) were dissolved in 150 mL H2O with 12.6 g dextran T70 (MW 70,000; Carl Roth GmbH & Co., KG, Karlsruhe, Germany). Iron hydroxide precipitated after dropwise addition of 150 mL 7.5% base ammonia (NH3) at room temperature. After heating the solution for 30 minutes to 60 minutes at 70°C, the iron hydroxide was reduced to iron oxide and Fe3O4 and dextran enclosed the particles. After synthesis, the particles were centrifuged for size separation and dialyzed against H2O. Several steps of separation and purification were performed to concentrate the nanoparticles.
3
Synthesis and Characterization of Metal Nanoparticles
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All reagents were of analytical purity and used without further purification. Iron(II) chloride tetrahydrate (FeCl2·4H2O, 99%), sodium hydroxide (NaOH, 99.99%), diethylene glycol (DEG, 99%), N-methyldiethanolamine (NMDEA, 99%), nitric acid (HNO3, 70%), copper(II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O ≥99.99%), polyvinylpyrrolidone (PVP, Mw 55 kDa), hydrazine hydrate (55%), ammonium sulfide solution ((NH4)2S, 20%), dihydrorhodamine 123 (DHR123 ≥95%), sodium cacodylatetrihydrate (≥98%), glutaraldehyde solution (25% in H2O), hydrogen peroxide solution (30 wt. % in H2O) and formalin solution (10%) were purchased from Sigma-Aldrich (France). Iron(III) chloride hexahydrate (FeCl3·6H2O, 99%) and ethanol were obtained from VWR (France). Alamar BlueTM cell viability reagent was purchased from Thermo-Fisher.
4
Synthesis and Functionalization of Iron Oxide Nanoparticles
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Iron(II) chloride tetrahydrate (FeCl2·4H2O, 99%), sodium hydroxide (NaOH, 99.99%), diethylene glycol (DEG, 99%), N-methyldiethanolamine (NMDEA, 99%), nitric acid (HNO3, 70%), copper(II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O, ≥99.99%), polyvinylpyrrolidone (PVP, Mw 55 kDa), poly(ethylene glycol) methyl ether thiol (PEG-SH, Mw 2 kDa) hydrazine hydrate (55%), ammonium sulfide solution ((NH4)2S, 20%), glutaraldehyde solution (25% in H2O), and sodium cacodylatetrihydrate (≥98%) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Iron(III) chloride hexahydrate (FeCl3·6H2O, 99%) and ethanol were obtained from VWR (Rosny-sous-Bois, France).
5
Optimizing Micronutrient Supplementation for Marine Organisms
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Sodium nitrate (plant and cell-culture tested), sodium molybdate dihydrate, copper (II) sulfate pentahydrate, cobalt (II) chloride hexahydrate, butylated hydroxytoluene (BHT) and triethylamine were purchased to Sigma-Aldrich (Arklow, Ireland). Iron (III) chloride hexahydrate, zinc sulphate heptahydrate, manganese (II) chloride tetrahydrate, potassium dihydrogen phosphate and HPLC grade methanol and ethanol 96% were purchased to VWR International Ltd (Dublin, Ireland). Thiamine HCl (vit. B1), biotin (vit. H), cyanocobalamin (vit. B12) and Supelco 37 Component FAME Mix were sourced from Sigma-Aldrich. Tropic Marine Pro-Reef marine salt (Tropic Marin, Wartenberg, Germany) were obtained from a local retailer (McGuire’s Garden Centre, Woodstown, Ireland). Carotenoid standards were obtained from CaroteNature GmbH (Müsingen, Switzerland). The Pierce BCA protein assay kit was obtained from Thermo Fisher Scientific (Dublin, Ireland). The commercial krill and Calanus oil supplements Prowise SUPERBAkrill Oil, Lot 1074600100 (Prowise Healthcare Ltd, London, UK) and Arctic Ruby (Calanus) Oil, Lot 1702071 (Calanus AS, Tromsø, Norway) were purchased online. HPLC grade methyl tert-butyl ether (MTBE) was supplied by Fisher Scientific (Dublin, Ireland).
6
Antioxidant Assay Protocol Optimization
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Magnesium(II) chloride hexahydrate, iron(III) chloride hexahydrate, copper(II) chloride dihydrate, 4 M sodium hydroxide (NaOH) stock solution, sodium chloride (NaCl), trisodium citrate dihydrate, ammonium acetate, methanol and the 2,2diphenyl-1-picrylhydrazyl (DPPH) radical were all purchased from VWR International (Radnor, USA) with analytical purity and were used as received. Aluminum(III) chloride hexahydrate and xanthine were acquired in analytical grade from Alfa Aesar (Karlsruhe, Germany). 2,2′-Bipyridyl (99%) and nitro blue tetrazolium (90%, NBT) chloride monohydrate were purchased from Acros Organics (Geel, Belgium). xanthine oxidase from bovine milk was purchased from Sigma-Aldrich. The sodium salt of poly(styrene sulfonate) (PSS, molecular mass of 10 kg mol -1 ) and 20 weight% aqueous poly(diallyldimethyl ammonium) chloride (PDADMAC, average molecular mass of 275 kg mol -1 ) solution were purchased from Sigma-Aldrich (Budapest, Hungary). Ultrapure water was produced by reverse osmosis and UV irradiation using a Puranity TU 3 + UV/UF system (VWR International).
7
Antioxidant Capacity Evaluation
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2,2 0 -Bipyridyl, ascorbic acid, trichloroacetic acid, DLdithiothreitol, Na 2 HPO 4 , NaH 2 PO 4 xH 2 0, N-Ethylmaleimide and citric acid monohydrate were from Sigma-Aldrich (Deisenhofen, Germany). Ortho-phosphoric acid 85%, Iron(III)chloride hexahydrate from VWR (Leuven, Belgium) and ethanol from by Fisher Scientific (Fair Lwan, NJ, USA).
Apple pur ee (brand: HIPP) and carrot pur ee (brand: POUCE) without added vitamin C were purchased in a local supermarket in Avignon/France.
Apple pur ee (brand: HIPP) and carrot pur ee (brand: POUCE) without added vitamin C were purchased in a local supermarket in Avignon/France.
8
Synthesis of Superparamagnetic Nanogels
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Iron (II) chloride tetrahydrate (FeCl 2 .4H 2 O), iron (III) chloride hexahydrate (FeCl 3 .6H 2 O), iron (III) nitrate nonahydrate (Fe (NO 3 ) 3 .9H 2 O), ammonia solution (NH 3 , 20 %), nitric acid (HNO 3 , 52.5 %) and hydrochloric acid (HCl, 37 %), diethyl ether and technical acetone were purchased from VWR for the preparation of superparamagnetic nanoparticles of maghemite (-Fe 2 O 3 ).
Poly(ethylene glycol) methyl ether methacrylate (OEGMA, Mn = 500 g.mol -1 ), di(ethylene glycol) methyl ether methacrylate (MEO 2 MA, Mn = 188.22 g.mol -1 ), poly(ethylene glycol) diacrylate (OEGDA, Mn = 250 g.mol -1 ), methacrylic acid (MAA), potassium persulfate (KPS), potassium hydroxide (KOH, flakes), nitric acid (HNO 3 , 52.5 %) were purchased from Sigma-Aldrich for the synthesis of bare and magnetic nanogels. Hepes hemisodium salt (dry powder) from Gibco, penicillin and Alamar Blue assay from Life Technologies were used for all the in vitro cell studies. All materials were used as received without any purification. Water was distilled and deionized.
Poly(ethylene glycol) methyl ether methacrylate (OEGMA, Mn = 500 g.mol -1 ), di(ethylene glycol) methyl ether methacrylate (MEO 2 MA, Mn = 188.22 g.mol -1 ), poly(ethylene glycol) diacrylate (OEGDA, Mn = 250 g.mol -1 ), methacrylic acid (MAA), potassium persulfate (KPS), potassium hydroxide (KOH, flakes), nitric acid (HNO 3 , 52.5 %) were purchased from Sigma-Aldrich for the synthesis of bare and magnetic nanogels. Hepes hemisodium salt (dry powder) from Gibco, penicillin and Alamar Blue assay from Life Technologies were used for all the in vitro cell studies. All materials were used as received without any purification. Water was distilled and deionized.
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