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2 protocols using recombinant murine ifn γ

1

Autophagy Regulation Pathway Assay

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The antibodies used in the present study are listed in table S1. Protein A+G agarose beads, LPS, recombinant murine IFN-γ, IL-4, and LysoTracker Red were obtained from Beyotime Biotechnology (Haimen, China), and the Ni2+-NTA agarose was purchased from QIAGEN (Dusseldorf, Germany). CHX was obtained from Calbiochem (508739; Darmstadt, Germany). Dimethyl sulfoxide (DMSO; D2650), MG-132 (C-2211), ammonium chloride (NH4Cl; A9434), 3-MA (M9281), CQ phosphate (PHR1258), and puromycin (P9620) were purchased from Sigma-Aldrich, China. Rapamycin (HY-10219) was obtained from MedChemExpress (Shanghai, China), Bafilomycin A1 (S1413) was purchased from Selleck (Houston, TX, USA), and EBSS was purchased from Gibco (Gaithersburg, MD, USA). A set of small interfering RNA of control (6568) and BECN1 (6246) was purchased from Cell Signaling Technology (Danvers, MA, USA). All other general reagents are commercially available and used as received.
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2

Macrophage Polarization Modulation Protocol

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Tetraethylorthosilicate (TEOS), Na2CO3, ethanol, methanol, ammoniumhydroxide (NH3·H2O), hexadecyl-trimethyl-ammoniumbromide (CTAB) and potassium permanganate (KMnO4) were purchased from SinopharmChemReagent Co., Ltd. (China). Metformin, lipopoly-saccharide (LPS) and Recombinant Murine IFN-γ were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Murine IL-4 was provided by PeproTech Biotechnology Co., Ltd. (Suzhou, China). Polyclonal antibodies CD47, CD80 and CD206 were obtained from Proteintech Group, Inc. (Wuhan, China). Coumarin-6 was ordered from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Mouse Tumor Necrosis Factor Alpha and Mouse IL-10 ELISA kit was provided by ABclonal Biotechnology Co., Ltd. (Wuhan, China). ELISA kits of Mouse iNOS and Mouse Arg-1 were obtained from Jonln Biotechnology Co., Ltd. (Shanghai, China). FITC-Anti-Mouse CD80 Antibody, FITC-Anti-Mouse CD206 and APC-Anti-Mouse CD206 Antibody were provided by Elabscience Biotechnology Co., Ltd. (Wuhan, China). β-Actin, AMPKα (D63G4) Rabbit mAb and Phospho-AMPKα (Thr172) (40H9) Rabbit mAb were ordered from Cell Signaling Technology, Inc. (MA, USA). DSPE-PEG-M2pep was purchased from SunLipo NanoTech Co., Ltd. (Shanghai, China).
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