Recombinant murine ifn γ

Manufactured by Beyotime

Sourced in China

Recombinant murine IFN-γ is a laboratory product that functions as the murine (mouse) version of the cytokine interferon-gamma. Interferons are signaling proteins involved in the immune response.

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Lab products found in correlation

Ni2 nta agarose, by Qiagen (1 mentions) Puromycin p9620, by Merck Group (1 mentions) Rapamycin hy 10219, by MedChemExpress (1 mentions) Interleukin 4 (il 4), by Beyotime (1 mentions) Bafilomycin a1 s1413, by Selleck Chemicals (1 mentions) Lipopolysaccharides (lps), by Beyotime (1 mentions) Protein a g agarose bead, by Beyotime (1 mentions) Lyso tracker red, by Beyotime (1 mentions) Metformin, by Beyotime (1 mentions) Cd206, by Proteintech (1 mentions) Apc anti mouse cd206 antibody, by Elabscience (1 mentions) Ampkα d63g4 rabbit mab, by Cell Signaling Technology (1 mentions) Phospho ampkα thr172 40h9 rabbit mab, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Lipopolysaccharide, by Beyotime (1 mentions)

2 protocols using recombinant murine ifn γ

Autophagy Regulation Pathway Assay

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The antibodies used in the present study are listed in table S1. Protein A+G agarose beads, LPS, recombinant murine IFN-γ, IL-4, and LysoTracker Red were obtained from Beyotime Biotechnology (Haimen, China), and the Ni²⁺-NTA agarose was purchased from QIAGEN (Dusseldorf, Germany). CHX was obtained from Calbiochem (508739; Darmstadt, Germany). Dimethyl sulfoxide (DMSO; D2650), MG-132 (C-2211), ammonium chloride (NH₄Cl; A9434), 3-MA (M9281), CQ phosphate (PHR1258), and puromycin (P9620) were purchased from Sigma-Aldrich, China. Rapamycin (HY-10219) was obtained from MedChemExpress (Shanghai, China), Bafilomycin A1 (S1413) was purchased from Selleck (Houston, TX, USA), and EBSS was purchased from Gibco (Gaithersburg, MD, USA). A set of small interfering RNA of control (6568) and BECN1 (6246) was purchased from Cell Signaling Technology (Danvers, MA, USA). All other general reagents are commercially available and used as received.

Li X., Xia Q., Mao M., Zhou H., Zheng L., Wang Y., Zeng Z., Yan L., Zhao Y, & Shi J. (2021). Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKKα stability via selective autophagy. Science Advances, 7(4), eabc5539.

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Macrophage Polarization Modulation Protocol

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Tetraethylorthosilicate (TEOS), Na₂CO₃, ethanol, methanol, ammoniumhydroxide (NH₃·H₂O), hexadecyl-trimethyl-ammoniumbromide (CTAB) and potassium permanganate (KMnO₄) were purchased from SinopharmChemReagent Co., Ltd. (China). Metformin, lipopoly-saccharide (LPS) and Recombinant Murine IFN-γ were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Murine IL-4 was provided by PeproTech Biotechnology Co., Ltd. (Suzhou, China). Polyclonal antibodies CD47, CD80 and CD206 were obtained from Proteintech Group, Inc. (Wuhan, China). Coumarin-6 was ordered from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Mouse Tumor Necrosis Factor Alpha and Mouse IL-10 ELISA kit was provided by ABclonal Biotechnology Co., Ltd. (Wuhan, China). ELISA kits of Mouse iNOS and Mouse Arg-1 were obtained from Jonln Biotechnology Co., Ltd. (Shanghai, China). FITC-Anti-Mouse CD80 Antibody, FITC-Anti-Mouse CD206 and APC-Anti-Mouse CD206 Antibody were provided by Elabscience Biotechnology Co., Ltd. (Wuhan, China). β-Actin, AMPKα (D63G4) Rabbit mAb and Phospho-AMPKα (Thr172) (40H9) Rabbit mAb were ordered from Cell Signaling Technology, Inc. (MA, USA). DSPE-PEG-M2pep was purchased from SunLipo NanoTech Co., Ltd. (Shanghai, China).

Chong L., Jiang Y.W., Wang D., Chang P., Xu K, & Li J. (2023). Targeting and repolarizing M2-like tumor-associated macrophage-mediated MR imaging and tumor immunotherapy by biomimetic nanoparticles. Journal of Nanobiotechnology, 21, 401.

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