Total proteins were extracted from the cells using RIPA buffer with protease inhibitors (Beyotime, Shanghai, China) on ice, subjected to SDS-PAGE and electrophoretically transferred to PVDF membranes (Merck Millipore, MA, USA). Membranes were incubated in 5% non fat milk dissolved in Tris-buffered saline (TBS) containing 0.1% Tween-20 for 2h at room temperature and then incubated with primary antibodies as follows: EPB41L3 (1:500, Abcam, Cambridge, MA, USA), GAPDH (1:2000, Proteintech, Wuhan, China), followed by incubation with appropriate correlated HRP-conjugated secondary antibody. Then the membranes were incubated with secondary antibodies (Proteintech, Wuhan, China) at room temperature for 2h. Immunoblots were visualized by enhanced chemiluminescence (ECL kit, Advansta, USA) and scanned using ECLTM chemiluminescence detection system (Pierce, USA).
Epb41l3
Manufactured by Abcam
Sourced in United States
EPB41L3 is a protein that belongs to the protein 4.1 family. It is involved in the regulation of cell membrane structure and cytoskeletal organization.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor, by Beyotime (1 mentions)
Ecl kit, by Advansta (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cyclin a, by Cell Signaling Technology (1 mentions)
Caspase 8, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Cyclin d, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Cyclin e, by Cell Signaling Technology (1 mentions)
2 protocols using epb41l3
1
Western Blot Analysis of Protein Expression
2
Western Blotting Antibodies and Parameters
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blotting was performed according to the standard protocol (21 (link)) with the following antibodies: EPB41L3 (1:2,000; Abcam, Cambridge, MA, USA), caspase-8 (1:1,000), caspase-9 (1:1,000), caspase-3 (1:1,000), CyclinA (1:2,000), CyclinD (1:1,000), CyclinE (1:1,000) (all from Cell Signaling Technology, Inc., Danvers, MA, USA), CyclinB1 (1:1,000), Cyclin-dependent kinase 1 (CDK1, also known as Cdc2; 1:1,000) (both from ProteinTech Wuhan Sanying Biotechnology, Wuhan, China). GAPDH (1:8,000; ProteinTech Wuhan Sanying Biotechnology) was used as loading control.
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