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Alpha amylase

Manufactured by MP Biomedicals
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Sourced in United States

Alpha-amylase is an enzyme that catalyzes the hydrolysis of alpha-1,4-glycosidic bonds in starch, glycogen, and related polysaccharides. It is a key component in the digestion and conversion of complex carbohydrates into smaller, more readily absorbable sugars.

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Lab products found in correlation

Bovine bile, by Merck Group (1 mentions) Sodium hydroxide (naoh), by ITW Reagents (1 mentions) Pancreatin from porcine pancreas 8usp, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Scharlab (1 mentions) Pepsin from porcine gastric mucosa, by Merck Group (1 mentions) Ethanol, by Scharlab (1 mentions) Magnesium chloride hexahydrate, by Scharlab (1 mentions) Sodium potassium tartrate, by Merck Group (1 mentions) Acarbose, by Merck Group (1 mentions) Xanthine, by Merck Group (1 mentions) Allopurinol, by Merck Group (1 mentions) Starch, by Merck Group (1 mentions)

2 protocols using alpha amylase

1

In Vitro Gastrointestinal Digestion

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Ethanol 96% (v/v) extra pure, sodium bicarbonate (NaHCO3), magnesium chloride hexahydrate (MgCl2·6H2O), ammonium carbonate ((NH4)2CO3), and hydrochloric acid (HCl) 37% (v/v) were purchased from Scharlau (Barcelona, Spain). Potassium chloride (KCl), potassium phosphate monobasic (KH2PO4), sodium chloride (NaCl), calcium chloride dihydrate (CaCl2·2H2O), and sodium hydroxide (NaOH) 1M were purchased from Panreac (Barcelona, Spain). Alpha-amylase used for in vitro simulated gastrointestinal digestion was purchased from MP Biomedicals (Aurora, Ohio, USA), and pepsin from porcine gastric mucosa, pancreatin from porcine pancreas (8USP), and bile bovine were purchased from Sigma Aldrich (Steinheim, Germany).
Reche C., Rosselló C., Eim V., León A.E, & Simal S. (2023). High-Power Ultrasound in Gas Phase: Effects on the Bioactive Compounds Release from Red Bell Pepper during In Vitro Gastrointestinal Digestion. Antioxidants, 12(2), 356.
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2

Extraction and Fractionation of Plant Compounds

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The solvents used for extraction and fractionation of plant material were of high-performance liquid chromatography (HPLC) grade. XO, xanthine, allopurinol, acarbose, and starch were purchased from Sigma-Aldrich, alpha-amylase from MP Biomedicals, sodium potassium tartrate from Merck and 3,5-dinitrosalisylic acid (DNS) from Fisher Scientific. All other reagents were of analytical grades.
Saeed R, & Ahmed D. (2015). Bioactive compounds from Carissa opaca roots and xanthine oxidase and alpha-amylase inhibitory activities of their methanolic extract and its fractions in different solvents. Pharmacognosy Research, 7(4), 295-301.
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