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2 protocols using sodium caseinate sc

1

Characterization of Milk Proteins and Nano-additives

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Bovine micellar casein and whey protein isolate were obtained by microfiltration as reported elsewhere [30] (link). Soy protein isolate (Premium Powders Pty Ltd), pea protein isolate (True Protein Pty Ltd), titanium dioxide (P25, ≥99.5%, 21 nm primary particle size, Sigma-Aldrich), two graphite materials (synthetic powder, <20 µm, Sigma-Aldrich; and natural flakes, −325 mesh, Sigma-Aldrich), chitosan (from shrimp shells, ≥75% deacetylated, Sigma-Aldrich), sodium caseinate (SC, Sigma-Aldrich), gold(III) chloride trihydrate (≥99.9%, Sigma-Aldrich), cyclohexane, decane, dodecane, tetradecane and hexadecane (≥99% for all alkanes, Sigma-Aldrich), sunflower oil, skim milk powder, butter (local supermarket), polyglycerol polyricinoleate (PGPR, Palsgaard A/S), single-layer graphene oxide suspension (SKU# GNO1W001, 10 mg mL−1, ACS Material, LLC) were used as received. Analytical grade chemicals, including sodium chloride, L-ascorbic acid, potassium permanganate, sulfuric acid, sodium hydroxide, hydrochloric acid, tri-potassium citrate, calcium chloride, dipotassium phosphate, were obtained from Chem-Supply. Milli-Q water was used in all experiments.
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2

Listeria monocytogenes Phage Preparation

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Listeria monocytogenes (CECT 934, ATCC 19114) pathogen strain was chosen for its impact on food-borne illness. The strain was maintained at −80 °C in a tryptone soy broth (TSB) supplemented with 20% glycerol. For experimental use, the stock culture was maintained by regular subculture at 4 °C on tryptone soy agar (TSA) and transferred monthly. Before use, a loopful of the strain was transferred to 10 mL of TSB and incubated at 37 °C for 24 h. All microbiological products were provided by Scharlab, Barcelona, Spain.
Bacteriophage preparation LISTEX™ P100 was obtained from MICREOS Food Safety Inc., Wageningen, The Netherlands. The bacteriophages were stored in the original suspension at 4 °C.
Polyvinyl alcohol (PVOH) was obtained from Gohsenol GH17, Nippon Synthetic Chemical Company, Osaka, Japan. Gelatin from porcine skin, type A, sodium caseinate (SC) (casein sodium salt from bovine milk), and sodium alginate (SA) were purchased from Sigma Aldrich (Madrid, Spain). Glycerol was obtained from Panreac Apllichem (Barcelona, Spain).
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