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Rpgrip1l

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RPGRIP1L is a gene that encodes a protein involved in the structure and function of the primary cilium, a specialized organelle found in many cell types. The RPGRIP1L protein plays a role in the assembly and maintenance of the primary cilium, which is important for various cellular processes, such as signal transduction, cell cycle regulation, and organ development. The RPGRIP1L gene is widely expressed in different tissues and has been associated with several genetic disorders that affect ciliary function.

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Lab products found in correlation

γ tubulin, by Abcam (2 mentions) Acetylated α tubulin, by Merck Group (2 mentions) Krt17, by Abcam (2 mentions) Bromodeoxyuridine (brdu), by Thermo Fisher Scientific (2 mentions) Phospho histone h3, by Cell Signaling Technology (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Krt14, by Fortrea (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions) Ds qi1mc camera, by Nikon (2 mentions)

2 protocols using rpgrip1l

1

Immunofluorescence and TUNEL Staining Protocol

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Immunofluorescence and TUNEL staining were performed as described previously (Dai et al., 2013 (link)). The following primary antibodies were used: RPGRIP1L, 1:100 (Origene, Rockville, MD); p63, 1:100 (Santa Cruz Biotechnology, Santa Cruz, CA); KRT14, 1:1,000 (Covance, Princeton, NJ); KRT1, 1:500 (Roop et al., 1987 (link)); KRT17, 1:1,000 (Abcam, Cambridge, MA); LOR, 1:100 (Covance); NGFR, 1:100 (Promega); LEF1, 1:100 (Cell Signaling, Danvers, MA); BrdU, 1:50 (Life Technologies); acetylated α-tubulin, 1:1,000 (Sigma, St. Louis, MO); γ-tubulin, 1:1,000 (Abcam); ARL13B, 1:1,000 (#73-287, NeuroMab, Davis, CA); phospho-histone H3, 1:100 (Cell Signaling). AlexaFluor-conjugated secondary antibodies (1:250) were from Life Technologies. Sections were sealed in mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Chen J., Laclef C., Moncayo A., Snedecor E.R., Yang N., Li L., Takemaru K.I., Paus R., Schneider-Maunoury S, & Clark R.A. (2014). The ciliopathy gene Rpgrip1l is essential for hair follicle development. The Journal of investigative dermatology, 135(3), 701-709.
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2

Immunofluorescence and TUNEL Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence and TUNEL staining were performed as described previously (Dai et al., 2013 (link)). The following primary antibodies were used: RPGRIP1L, 1:100 (Origene, Rockville, MD); p63, 1:100 (Santa Cruz Biotechnology, Santa Cruz, CA); KRT14, 1:1,000 (Covance, Princeton, NJ); KRT1, 1:500 (Roop et al., 1987 (link)); KRT17, 1:1,000 (Abcam, Cambridge, MA); LOR, 1:100 (Covance); NGFR, 1:100 (Promega); LEF1, 1:100 (Cell Signaling, Danvers, MA); BrdU, 1:50 (Life Technologies); acetylated α-tubulin, 1:1,000 (Sigma, St. Louis, MO); γ-tubulin, 1:1,000 (Abcam); ARL13B, 1:1,000 (#73-287, NeuroMab, Davis, CA); phospho-histone H3, 1:100 (Cell Signaling). AlexaFluor-conjugated secondary antibodies (1:250) were from Life Technologies. Sections were sealed in mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Chen J., Laclef C., Moncayo A., Snedecor E.R., Yang N., Li L., Takemaru K.I., Paus R., Schneider-Maunoury S, & Clark R.A. (2014). The ciliopathy gene Rpgrip1l is essential for hair follicle development. The Journal of investigative dermatology, 135(3), 701-709.
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