Whatman 50 filter paper

Manufactured by Cytiva

The Whatman 50 filter paper is a high-quality cellulose-based filtration product designed for general laboratory applications. It is characterized by its medium filtration speed and medium particle retention, making it suitable for a variety of filtering tasks. The Whatman 50 filter paper is available in a range of sizes to accommodate different laboratory needs.

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Lab products found in correlation

Casein hydrolysate, by Merck Group (2 mentions) Whatman 1 filter paper, by Cytiva (2 mentions)

6 protocols using whatman 50 filter paper

Qualitative Virus Preparation Analysis

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Example 3

For qualitative analysis of virus preparations, electron microscopy pictures were taken. For this, 5 μl virus suspension was added to a ready-to-use carbon-coated copper grid and incubated for 2 min. The grid was then washed with 5 μl bidest water and coated with 2% uranyl acetate for 30 s. The drops were absorbed from the grid with Whatman 50 filter paper and the grid was dried for approximately 1 min. Photos were taken with a Zeiss transmission electron microscope at 20,100× magnification.

US11324788B2. Parvovirus formulation for treating tumors (2022-05-10). DEUTSCHES KREBSFORSCHUNGSZENTRUM [DE], RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG [DE]. Inventors: Barbara Leuchs [DE], Karsten Geletneky [DE], Jean Rommelaere [DE], Christiane Dinsart [DE], Michael Dahm [DE], Ottheinz Krebs [DE].

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Trichoderma atroviride Inoculum Cultivation

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A Trichoderma atroviride inoculum was plated on the center of a PDYC (24 g l⁻¹ potato dextrose broth (Difco, UK), 1.2 g l⁻¹ casein hydrolysate (Sigma-Aldrich), 2 g l⁻¹ yeast extract (Difco) agar plate and incubated for twenty-four hours in dark conditions. 3 ml of PDYC media were subsequently added to a new 10 cm culture plate. In the center of the plate a Whatman 50 filter paper cut to a diameter of 9 cm was placed over an 8 cm Whatman 1 filter paper. On top of these, a 0.5 cm square from the periphery of the fungal growth on the agar plate was placed in the center and incubated in the dark at room temperature for 48 h.

Adir O., Albalak M.R., Abel R., Weiss L.E., Chen G., Gruber A., Staufer O., Kurman Y., Kaminer I., Shklover J., Shainsky-Roitman J., Platzman I., Gepstein L., Shechtman Y., Horwitz B.A, & Schroeder A. (2022). Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nature Communications, 13, 2328.

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Negative Staining of SVA Nanoparticles

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Samples from both the 20% and 35% sucrose fractions of PEG-precipitated LF-BK α_Vβ₆ propagated SVA were evaluated by negative staining on a Hitachi 7600 transmission electron microscope with a 2 k × 2 k AMT camera at 80 kV. Prior to application of sample, 300-mesh formvar/carbon coated grids were inverted on 1% alcian blue for 5 min followed by a rinse in ddH₂O. Samples were adhered at a 1:5 dilution for 5 min; 2% uranyl acetate was added while blotting across Whatman 50 filter paper.

Mason J., Primavera V., Martignette L., Clark B., Barrera J., Simmons J., Hurtle W., Neilan J.G, & Puckette M. (2022). Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK αVβ6 Cell Line for Senecavirus A Research. Viruses, 14(9), 1875.

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Electron Microscopy of Viral Preparations

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Example 3

US10624935B2. Parvovirus formulation for treating tumors (2020-04-21). DEUTSCHES KREBSFORSCHUNGSZENTRUM [DE], RUPRECHT-KARLS-UNIVERSITÄT HEIDELBERG [DE]. Inventors: Barbara Leuchs [DE], Karsten Geletneky [DE], Jean Rommelaere [DE], Christiane Dinsart [DE], Michael Dahm [DE], Ottheinz Krebs [DE].

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Quantifying DNA Synthesis via [3H]Thymidine

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The liver and kidneys were promptly removed, washed and 1 G of liver and kidney were homogenized in ice-cold water (10% w/v). The precipitate was incubated overnight at 4°C with cold PCA (10%) after rinsing with cold TCA (5%). It was centrifuged after incubation, and the precipitate was rinsed with 5% of cold PCA. All centrifugations had been done at 4°C up to this point. The precipitate was dissolved in warm PCA (10%), incubated for 30 min in a boiling water bath and then filtered using Whatman 50 filter paper. By adding the scintillation fluid to the filtrate, it was employed for [ 3 H]thymidine incorporation counting in a liquid scintillation counter. DPM/g DNA was used to calculate the quantity of [ 3 H]thymidine incorporated. 33, (link)34

Ahmed A.R., Vun-Sang S, & Iqbal M. (2022). Therapeutic role of nitroglycerin against copper-nitrilotriacetate induced hepatic and renal damage. Human & experimental toxicology, 41.

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Trichoderma atroviride Growth Protocol

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A Trichoderma atroviride inoculum, was plated on the center of a PDYC (24 g l -1 potato dextrose broth (Difco, UK), 1.2 g l -1 casein hydrolysate (Sigma-Aldrich), 2 g l -1 yeast extract (Difco) agar plate and incubated for twenty-four hours in dark conditions. 3 ml of PDYC media were subsequently added to a new 10 cm culture plate. In the center of the plate a Whatman 50 filter paper cut to a diameter of 9 cm was placed over an 8 cm Whatman 1 filter paper. On top of these, a 0.5 cm square from the periphery of the fungal growth on the agar plate was placed in the center and incubated in the dark at room temperature for 48 hours.

Adir O., Abel R., Albalak M.R., Weiss L.E., Chen G., Gruber A., Staufer O., Shklover J., Shainsky‐Roitman J., Platzman I., Gepstein L., Shechtman Y., Horwitz B.A., & Schroeder A. (2021). Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins.

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