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Prominence 20 system

Manufactured by Shimadzu
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The Prominence 20 System is a high-performance liquid chromatography (HPLC) instrument developed by Shimadzu. It is designed for efficient and reliable separation and analysis of a wide range of chemical compounds. The system features a modular design, allowing for customization to meet specific analytical requirements.

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Lab products found in correlation

Tsq quantum, by Thermo Fisher Scientific (2 mentions) Xcalibur qual browser, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Prominence system (67 citations) LC-20 Prominence HPLC system (11 citations) LC-20 Prominence system (11 citations) Prominence LC-20 AB HPLC system (4 citations) LC-20 Prominence BioInert HPLC system (2 citations) LC-20 AD Prominence Liquid Chromatograph system (2 citations) Prominence 20 UFLCXR system (2 citations) Prominence 20 A series UFLC System (2 citations) LC-20 Prominence Chromatographic System (2 citations)

4 protocols using prominence 20 system

1

Cardiac Metabolite Profiling by LC-MS/MS

Cited 1 time
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Heart powder was serially extracted in 80% methanol, evaporated under N2, and resuspended in 50% methanol. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was essentially as previously described (Nadtochiy et al., 2015 (link)). Metabolites were resolved by HPLC (Shimadzu Prominence 20 System) and identified by single-reaction monitoring on a triple-quadrupole mass spectrometer (Thermo Quantum TSQ) (see the Supplemental Experimental Procedures for details). Data were analyzed using MzRock (https://code.google.com/archive/p/mzrock/). Selected metabolites were also analyzed using XCalibur Qual Browser (Thermo Scientific). Data were normalized to protein content.
Zhang J., Wang Y.T., Miller J.H., Day M.M., Munger J.C, & Brookes P.S. (2018). Accumulation of Succinate in Cardiac Ischemia Primarily Occurs via Canonical Krebs Cycle Activity. Cell reports, 23(9), 2617-2628.
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2

Cardiac Metabolite Profiling by LC-MS/MS

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Heart powder was serially extracted in 80% methanol, evaporated under N2, and resuspended in 50% methanol. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was essentially as previously described (Nadtochiy et al., 2015 (link)). Metabolites were resolved by HPLC (Shimadzu Prominence 20 System) and identified by single-reaction monitoring on a triple-quadrupole mass spectrometer (Thermo Quantum TSQ) (see the Supplemental Experimental Procedures for details). Data were analyzed using MzRock (https://code.google.com/archive/p/mzrock/). Selected metabolites were also analyzed using XCalibur Qual Browser (Thermo Scientific). Data were normalized to protein content.
Zhang J., Wang Y.T., Miller J.H., Day M.M., Munger J.C, & Brookes P.S. (2018). Accumulation of Succinate in Cardiac Ischemia Primarily Occurs via Canonical Krebs Cycle Activity. Cell reports, 23(9), 2617-2628.
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3

Quantitative HPLC Analysis of Heart Metabolites

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Heart powder was serially extracted in 8% perchloric acid. Metabolites were resolved on high-performance liquid chromatography (HPLC) (Shimadzu Prominence 20 System) with photodiode array detection, quantified via bracket-calibration with a standard curve (see the Supplemental Experimental Procedures for details), and normalized to protein.
Zhang J., Wang Y.T., Miller J.H., Day M.M., Munger J.C, & Brookes P.S. (2018). Accumulation of Succinate in Cardiac Ischemia Primarily Occurs via Canonical Krebs Cycle Activity. Cell reports, 23(9), 2617-2628.
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4

Quantitative HPLC Analysis of Heart Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heart powder was serially extracted in 8% perchloric acid. Metabolites were resolved on high-performance liquid chromatography (HPLC) (Shimadzu Prominence 20 System) with photodiode array detection, quantified via bracket-calibration with a standard curve (see the Supplemental Experimental Procedures for details), and normalized to protein.
Zhang J., Wang Y.T., Miller J.H., Day M.M., Munger J.C, & Brookes P.S. (2018). Accumulation of Succinate in Cardiac Ischemia Primarily Occurs via Canonical Krebs Cycle Activity. Cell reports, 23(9), 2617-2628.
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