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2 protocols using alexfluor 488 anti rabbit

1

Immunofluorescence Antibody Validation

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The following primary antibodies were used: rabbit polyclonal hnRNP M (Abcam, #177957), rabbit polyclonal Histone 3 (Abcam, #1791), mouse monoclonal Beta-Actin (Abcam, #6276), mouse monoclonal hnRNP L (Abcam, #6106–100), rabbit polyclonal Beta-Tubulin (Abcam, #179513), mouse monoclonal hnRNP U (Santa-Cruz, sc-32315), DAPI nuclear staining (Thermo Fisher), and mouse monoclonal ANTI-FLAG M2 antibody (Sigma-Aldrich, F3165). Secondary antibodies used were asfollows: IR Dye CW 680 goat anti-rabbit, IR Dye CW800 goat anti-mouse (LI-COR), Alexfluor-488 anti-rabbit and Alexafluo-647 anti-mouse secondary antibodies for immunofluorescence (LI-COR).
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2

Immunofluorescence Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used: rabbit polyclonal hnRNP M (Abcam, #177957), rabbit polyclonal Histone 3 (Abcam, #1791), mouse monoclonal Beta-Actin (Abcam, #6276), mouse monoclonal hnRNP L (Abcam, #6106–100), rabbit polyclonal Beta-Tubulin (Abcam, #179513), mouse monoclonal hnRNP U (Santa-Cruz, sc-32315), DAPI nuclear staining (Thermo Fisher), and mouse monoclonal ANTI-FLAG M2 antibody (Sigma-Aldrich, F3165). Secondary antibodies used were asfollows: IR Dye CW 680 goat anti-rabbit, IR Dye CW800 goat anti-mouse (LI-COR), Alexfluor-488 anti-rabbit and Alexafluo-647 anti-mouse secondary antibodies for immunofluorescence (LI-COR).
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