To assay inhibition of mice PCSK9 by vaccine-induced antibodies, interaction of induced antibodies with PCSK9 was evaluated. For this purpose, the same kit CircuLex rat PCSK9 ELISA was used, but for HRP-conjugated anti-PCSK9 antibody, detection was performed with HRP-conjugated anti-mouse IgG (H + L) (Sigma Aldrich; dilution 1 : 5000) incubated for 1 h at RT, followed by the substrate reagent and stop solution provided by the supplier. The OD was detected at 450 nm with the Microwell plate reader.
Hrp conjugated anti mouse igg h l
Manufactured by Merck Group
HRP-conjugated anti-mouse IgG (H + L) is a laboratory reagent that consists of horseradish peroxidase (HRP) conjugated to antibodies specific to mouse immunoglobulin G (IgG), including both the heavy (H) and light (L) chains. This product is commonly used in various immunoassay techniques, such as enzyme-linked immunosorbent assay (ELISA), to detect and quantify the presence of mouse IgG in samples.
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Lab products found in correlation
2 protocols using hrp conjugated anti mouse igg h l
1
Evaluating PCSK9 Inhibition by Vaccine Antibodies
2
Evaluation of PCSK9 Inhibition by Vaccine-Induced Antibodies
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To evaluate inhibition of mice PCSK9 by vaccine-induced antibodies, interaction of generated-antibodies with PCSK9 was assayed. For this purpose, the same kit CircuLex rat PCSK9 ELISA was used, but in turn of HRP-conjugated anti-PCSK9 antibody, detection was performed with HRP-conjugated anti-mouse IgG (H+L) (Sigma Aldrich; dilution 1 : 5000) incubated for 1 h at RT, followed by the substrate reagent and stop solution provided by the supplier. The OD was detected at 450 nm with the Microwell plate reader.
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