Anti phospho akt ser473 mouse mab

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-phospho-AKT (Ser473) mouse MAb is a monoclonal antibody that specifically recognizes the phosphorylated form of the serine 473 residue of the AKT protein. AKT is a key regulator of cellular processes such as growth, proliferation, and survival.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated goat anti mouse and anti rabbit igg, by Santa Cruz Biotechnology (2 mentions) 12 o tetradecanoylphorbol 13 acetate tpa, by Merck Group (1 mentions) Doxycycline hyclate, by Merck Group (1 mentions) Anti akt rabbit pab, by Cell Signaling Technology (1 mentions) Anti his rabbit mab, by Cell Signaling Technology (1 mentions) Anti cxcr2 rabbit pab, by Santa Cruz Biotechnology (1 mentions) Sodium butyrate, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Anti flag m2 rabbit mab, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Mouse anti-phospho-Akt Ser473 Anti-phospho-Akt (Ser473) Phospho-Akt (Ser473) Mouse anti-phospho Akt (Ser473) Akt Anti-Akt-mAb Anti-phospho-Akt Mouse anti-Akt Phospho-Akt Anti-AKT

2 protocols using anti phospho akt ser473 mouse mab

Antibodies for KSHV Detection

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Anti-KSHV vIL-6 rabbit polyclonal antibody (PAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA). Anti-KSHV ORF65 mouse monoclonal antibody (MAb) was used to detect KSHV capsids [68 (link)]. Anti-KSHV RTA peptide antibodies were generated by immunization of rabbits with RTA peptide as previously described [69 (link)]. Anti-phospho-AKT (Ser473) mouse MAb, anti-AKT rabbit PAb, anti-GRK2 rabbit PAb, anti-CD31 mouse MAb and anti-His rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-CXCR2 rabbit PAb, anti-α-tubulin and GAPDH mouse MAbs, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-smooth muscle actin (SMA) rabbit PAb were obtained from AbbiotecTM (San Diego, CA, USA). 12-O-tetradecanoylphorbol-13-acetate (TPA; 20 ng/mL), doxycycline hyclate (1 μg/ml) and sodium butyrate (1 mM) were obtained from Sigma (St. Louis, MO, USA). 4′, 6′-diamidino-2-phenylindole (DAPI) was purchased from Beyotime Institute of Biotechnology (Nantong, China).

Li W., Jia X., Shen C., Zhang M., Xu J., Shang Y., Zhu K., Hu M., Yan Q., Qin D., Lee M.S., Zhu J., Lu H., Krueger B.J., Renne R., Gao S.J, & Lu C. (2016). A KSHV microRNA enhances viral latency and induces angiogenesis by targeting GRK2 to activate the CXCR2/AKT pathway. Oncotarget, 7(22), 32286-32305.

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Protein Expression Analysis by Western Blot

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Western blot was performed as previously described (17 (link)). Anti-vascular endothelial growth factor (VEGF) rabbit polyclonal antibody (pAb), anti-glyceraldehyde-3-phosphate dehydrogenase mouse monoclonal antibody (mAb), anti-α-Tubulin mouse mAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Anti-Flag M2 rabbit mAb, anti-phospho-PTEN (Ser380) rabbit pAb, anti-PTEN rabbit pAb, anti-phospho-phosphatidylinositol 3-kinase (PI3K) [p85(Tyr458)/p55(Tyr199)] rabbit pAb, anti-PI3K rabbit pAb, anti-phospho-AKT (Ser473) mouse mAb, anti-AKT mouse mAb, anti-phospho-mTOR (Ser2448) rabbit pAb and anti-His mouse mAb were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-smooth muscle actin (SMA) rabbit pAb was purchased from Abbiotec™ (San Diego, CA, USA). Anti-green fluorescent protein (GFP) mouse mAb was purchased from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China).

Xue M., Yao S., Hu M., Li W., Hao T., Zhou F., Zhu X., Lu H., Qin D., Yan Q., Zhu J., Gao S.J, & Lu C. (2014). HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway. Nucleic Acids Research, 42(15), 9862-9879.

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