35S (CaMV35S) promoter-driven expression clones were generated in the pSGFP vectors, resulting in N-terminal green fluorescent protein (GFP)-protein fusions. The CDS of V. sativa PCS1 gene amplified using the primers of PCS1F1 and PCS1R1. PCR products were cloned into pSGFP vector and confirmed by sequencing. 35S::VsPCS1-GFP plasmid were transiently expressed in V. sativa mesophyll protoplasts via polyethylene glycol (4000)-calcium transfection (Yoo et al., 2007 (link)). Incubating at room temperature for 16 h, transformed cells were observed with a uitraviewvox confocal microscope (PerkinElmer, United States).
Uitraviewvox confocal microscope
Manufactured by PerkinElmer
Sourced in United States
The Ultraviewvox confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It provides a core function of capturing high-resolution, real-time images of biological samples with minimized out-of-focus light, enabling detailed analysis of cellular and subcellular structures.
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Lab products found in correlation
2 protocols using uitraviewvox confocal microscope
1
Transient Expression of VsPCS1-GFP in Vicia sativa
2
In Vivo Cd Localization in Protoplasts
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The leaf protoplasts with or without Cd treatment from WT, atpcs1, 35S::VsPCS1, and 35S::VsPCS1/atpcs1 were loaded with 0.04% v/v Leadmium Green AM dye (Molecular Probes, Invitrogen, Carlsbad, CA, United States). Intracellular Cd localization was observed according to the method of Park et al. (2012) (link) using a uitraviewvox confocal microscope (PerkinElmer, United States). The intensity of green fluorescence signal was quantified by ImageJ software.
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