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1 2 distearoyl sn glycero 3 phosphoethanolamine n amino polyethylene glycol 2000 dspe peg

Manufactured by Lipoid
Sourced in Germany

1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(amino[polyethylene glycol]-2000) (DSPE-PEG) is a synthetic lipid compound. It consists of a phosphatidylethanolamine (PE) head group, two stearic acid (C18:0) acyl chains, and a polyethylene glycol (PEG) moiety attached to the PE head group.

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2 protocols using 1 2 distearoyl sn glycero 3 phosphoethanolamine n amino polyethylene glycol 2000 dspe peg

1

Lipid-based Nanocarrier Synthesis

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Medium-chain triglycerides (MCTs), 1,2-dioleoyl- sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-trimethylammonium propane (DOTAP), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(amino[polyethylene glycol]-2000) (DSPE-PEG) were purchased from Lipoid (Ludwigshafen, Germany). Glycerol and chloroform were obtained from Merck (Rio de Janeiro, Brazil). 4-Methylumbelliferylalpha-l-iduronide was purchased from Glycosynth (Warrington, UK), and TRIzol® Reagent was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Platinum SYBR Green qPCR SuperMix-UDG kit was obtained from Thermo Fisher Scientific, and High-Capacity cDNA Reverse Transcription Kit was purchased from Thermo Fisher Scientific. All other chemicals were of analytical grades.
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2

Purification and Characterization of His-Tagged Proteins

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Tris-base, imidazole, sodium phosphate and sodium chloride were obtained from Sigma-Aldrich. 1-AMA was purchased from TCI chemicals. Nickel Magnetic Beads for His Tag Protein Purification was available from Biotool, Bimake and Molecular weight Precision Plus Protein TM standards were purchased from BioRad. Culture medium was purchased from GRISP. 1,2-Dioleoylsn-glycero-3-phosphoethanolamine (DOPE), and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) were purchased from Lipoid. Cholesterol was acquired from Sigma-Aldrich. Fluorescein isothiocyanate (FITC) was acquired from Sigma-Aldrich and Alexa Fluor 467 was purchased from ThermoFisher Scientific. All other reagents were acquired from Sigma-Aldrich and used as received.
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