Cells (5000 cells/well) were seeded on poly-DL-ornithine (Sigma-Aldrich) coated 96-well plates (10 µg/mL) and allowed to grow for 24 h before treatment with BAL27862. Growth inhibition of cells was measured after 72 h by using a sulforhodamine B assay kit (Sigma-Aldrich) as described previously [19 (link)].
Sulforhodamine b assay kit
Manufactured by Merck Group
The Sulforhodamine B assay kit is a colorimetric assay used for the quantitative determination of cellular protein content. The assay relies on the ability of the sulforhodamine B dye to bind to proteins under acidic conditions. The intensity of the resulting colored complex is proportional to the protein content of the sample, which can then be measured using a spectrophotometer.
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3 protocols using sulforhodamine b assay kit
1
Cytotoxicity Assay of BAL27862
2
Cell Growth Quantification by SRB Assay
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For 2D assay, cells were seeded at 5000 cells/well in 96-well plates and grown for 1 to 6 days. Each assay was performed in five replicates. For indicated time points, cells were fixed with 50% trichloroacetic acid at 4°C for 1 hour, and processed using the SulfoRhodamine B assay kit (Sigma).
3
BAL27862 Growth Inhibition Assay
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Cells (5,000 cells/well) were seeded on poly-DL-ornithine (Sigma-Aldrich) coated 96-well plates (10 mg/mL) and allowed to grow for 24 hours before treatment with BAL27862. Growth inhibition of cells was measured after 72 hours by using a sulforhodamine B assay kit (Sigma-Aldrich) as described previously (21) .
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