U0126 hy 12031

Manufactured by MedChemExpress

Sourced in China, United States

U0126 (HY-12031) is a highly selective and potent inhibitor of the dual-specificity mitogen-activated protein kinase kinases MEK1 and MEK2. It functions by blocking the activation of the ERK/MAPK signaling pathway.

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Lab products found in correlation

Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Ab179461, by Abcam (1 mentions) 18 1 lpa l7260, by Merck Group (1 mentions) One step tunel apoptosis assay kit c1080, by Beyotime (1 mentions) Anti p erk1 2 antibody ap0472, by ABclonal (1 mentions) Hy 18075, by MedChemExpress (1 mentions) Sb203580 hy 10256, by MedChemExpress (1 mentions) Anti gapdh antibody 10494 1 ap, by Proteintech (1 mentions) Cell mitochondria isolation kit c3601, by Beyotime (1 mentions) Sybr green qpcr master mix, by Vazyme (1 mentions) Jnk sc 7345, by Santa Cruz Biotechnology (1 mentions) Dulbecco s modified eagle s med, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Chloroquine c6628, by Merck Group (1 mentions) Trpc6 acc 017, by Alomone (1 mentions) Erk1 2 4695, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated goat anti rabbit antibody, by Beyotime (1 mentions) Dab sa hrp tunel cell apoptosis detection kit, by Wuhan Servicebio Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions)

6 protocols using u0126 hy 12031

Molecular Mechanisms of Apoptosis Regulation

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RevertAid First Strand cDNA Synthesis Kit was purchased from Thermo Fisher, Inc. qPCR SYBR Green Master Mix (Q141) was purchased from Vazyme. 18:1 LPA (L7260) was purchased from Sigma-Aldrich. One step TUNEL apoptosis assay kit (C1080), cell mitochondria isolation kit (C3601), and Rhodamine123 (C2007) were purchased from Beyotime. Anti-Bcl2 antibody (ab182858), anti-Bax antibody (ab32503), anti-p-JNK antibody (ab124956), and anti-JNK antibody (ab179461) were purchased from Abcam. Anti-p-ERK1/2 antibody (AP0472), anti-ERK1/2 antibody (A10613), anti-p-p38 antibody (AP0056), and anti-p38 antibody (A10832) were purchased from ABclonal. Anti-GAPDH antibody (10494-1-AP), anti-cleaved caspase-3 antibody (66470-2-Ig), and anti-COXIV antibody (11242-1-AP) were purchased from Proteintech. Cell Counting Kit-8 (CCK-8, HY-K0301), LPA1 receptor antagonist (AM095, HY-16039; BMS986020, HY-100619), LPA2 receptor antagonist (HY-18075), ERK1/2 inhibitor (U0126, HY-12031), p38 inhibitor (SB203580, HY-10256), and JNK inhibitor (SP600125, HY-12041) were purchased from MedChemExpress.

Zhang J., Li Y., Wang C., Wang Y., Zhang Y., Huang L, & Zhang Z. (2020). Lysophosphatidic Acid Induces Apoptosis of PC12 Cells Through LPA1 Receptor/LPA2 Receptor/MAPK Signaling Pathway. Frontiers in Molecular Neuroscience, 13, 16.

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Antibody and Drug Reagents for Neurodegenerative Research

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The following antibodies were used: TH (AB152) and spectrin (#Mab 1622) from Millipore; Iba1 (#01919741) from Wako; HA (H6908) and GFAP (G3893) from Sigma; Ago2 (sc-32877), Drosha (sc-33778), DICER (sc-30226), ubquitin (sc-8017), phosphorylated JNK (sc-6254), and JNK (sc-7345) from Santa Cruz Biotechnology; Caspase-3 (9662), cleaved Caspase-3 (#9664 L), ERK1/2 (9102), phosphorylated ERK (9101S), phosphorylated p38 (9216S), and p38 (8690S) from Cell Signaling Technology; phospho Serine (37430) from Qiagen; CD11b-Alexa Fluor 488 (557672) and CD45-APC (559864) from BD Biosciences; and GLAST-APC (130-098-803) from MiltenyiBiotec.
The following drugs were used: lipopolysaccharide (LPS, L2630), MPP⁺ (D048), MPTP (M0898), calpeptin (C8999) or MDL28170 (M6690), N-carbobenzoxyl-l-leucyl-l-leucyl-norvalinal (MG115, C6706), and lactacystin (L6785) from Sigma; U0126 (HY-12031), SP600125 (HY-12041), and SB203580 (HY-10256A) from Medchemexpress; and Z-DEVD-FMK (S7312) from Selleck.

Wang Q., He Q., Chen Y., Shao W., Yuan C, & Wang Y. (2018). JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss. Journal of Neuroinflammation, 15, 184.

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Cell Line Authentication and Maintenance for Renal Cancer Research

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786‐O (RRID: CVCL_1051), A‐498 (RRID: CVCL_1056), OS‐RC‐2 (RRID: CVCL_1626) and Caki‐1 (RRID: CVCL_0234) cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China). A‐498 and OS‐RC‐2 cell lines authentication was performed using STR profiling within the last 3 years at Shanghai Biowing Applied Biotechnology Co., Ltd. (Shanghai, China). 786‐O and Caki‐1 cell lines authentication was performed using STR profiling within the last 3 years at Zhejiang University Forensic Science Center. All cell lines were routinely tested and shown to be mycoplasma‐free as determined by the polymerase chain reaction (PCR)‐based method. All experiments were performed with mycoplasma‐free cells. A‐498 and OS‐RC‐2 were cultured in Dulbecco's modified Eagle's Med (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Gibco, Australia); 786‐O and Caki‐1 were cultured in Roswell Park Memorial Institute (RPMI) 1640 (Invitrogen, Grand Island, NY) containing 10% FBS. All cells were grown as a monolayer on plastic cell culture dishes at 37°C in a humidified atmosphere containing 5% CO₂.
L‐2HG octyl esters (HY‐103641A), decitabine (HY‐A0004) and U0126 (HY‐12031) were purchased from the MedChemExpress (MCE, China). Diacetyl‐L‐tartaric anhydride (DATAN, 358924), L‐2HG (90790) and R‐2HG (H8378) were purchased from Sigma‐Aldrich (St. Louis).

Wang H., Wang L., Zheng Q., Lu Z., Chen Y., Shen D., Xue D., Jiang M., Ding L., Zhang J., Wu H., Xia L., Qian J., Li G, & Lu J. (2021). Oncometabolite L‐2‐hydroxyglurate directly induces vasculogenic mimicry through PHLDB2 in renal cell carcinoma. International Journal of Cancer, 148(7), 1743-1755.

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Neuronal Protein Interaction Assays

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Rabbit anti‐NOS1AP (sc‐9138) and mouse antigreen fluorescent protein (sc‐101525) were purchased from Santa Cruz (USA, Dallas). Rabbit anti‐nNOS (#4231), β‐amyloid (1‐42 Specific) (D9A3A) rabbit mAb #14974, Synapsin‐1 (D12G5) XP^® Rabbit mAb (#5297), rabbit anti‐CREB (#9197), rabbit anti‐ERK1/2 (#4695), mouse anti‐pERK1/2 (#5726), and mouse anti‐pCREB (#9196) were purchased from CST (Danvers, MA). Rabbit anti‐BDNF (ab108319), mouse anti‐HIV1 tat antibody [N3] (ab63957), rabbit anti‐TrkB (ab187041), and rabbit anti‐pTrkB (ab109684) were purchased from Abcam (Cambridge MA). Rabbit anti‐Dexras1 (ab15794) and mouse anti‐PSD95 (MAB1596) were purchased from Millipore (Danvers, MA). Zlc002, a small‐molecule inhibitor of nNOS–CAPON interaction, was designed and synthesized in our laboratory (Zhu et al., 2014). H89 (HY‐15979) and U0126 (HY‐12031) were purchased from MedChem Express. TrkB‐Fc was purchased from Sino Biological. Other chemical reagents were purchased from Sigma‐Aldrich and Thermo Fisher. TAT‐CAPONi (YGRKKRRQRRRELGDSLDDEIAV), TAT‐CAPONi/A22D (YGRKKRRQRRRELGDSLDDEIDV), and amyloid‐β_1‐42 (purity >95%) were purchased from GL Biochem (Shanghai) Ltd.

Zhang Y., Zhu Z., Liang H., Zhang L., Zhou Q., Ni H., Luo C, & Zhu D. (2018). nNOS–CAPON interaction mediates amyloid‐β‐induced neurotoxicity, especially in the early stages. Aging Cell, 17(3), e12754.

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Signaling Pathways Regulation Protocol

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The primary antibodies against Akt (9272), p-Akt (Ser473) (4060 P), cleaved caspase-3 (9661), p-p70S6K (9205), p70S6K (9202), p-ERK1/2 (4370), and ERK1/2 (4695) were purchased from Cell Signaling Technology. The primary antibodies against TRPC3 (ACC-016) and TRPC6 (ACC-017) were purchased from Alomone. The primary antibody against LC3B (L7543) was purchased from Sigma-Aldrich. The primary antibody against β-actin (TA-09) was purchased from the ZhongShan Biotechnology Company. The secondary antibodies HRP-conjugated anti-rabbit IgG (110777) and anti-mouse IgG (117228) were purchased from the KeRui Biotechnology Company. H₂O₂ (107298) was purchased from Millipore. Chloroquine (C6628) and t-BOOH (458139) were purchased from Sigma-Aldrich. SAR7334 (HY-15699), bafilomycin A1 (HY-100558), MK2206 (HY-10358), and U0126 (HY-12031) were purchased from MedChemExpress.

Hou X., Xiao H., Zhang Y., Zeng X., Huang M., Chen X., Birnbaumer L, & Liao Y. (2018). Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. Cell Death & Disease, 9(10), 1015.

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Investigating Cisplatin-Induced Apoptosis

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IQC (HY-N0768) and U0126 (HY-12031) were purchased from MedChemExpress (MJ, NJ, United States). Cisplatin was procured from Sigma Aldrich (St. Louis, MO, United States). Periodic Acid-Schiff (PAS) stain kit was ordered from Servicebio (G1008, Wuhan, China). Antibodies against Bax, Bcl-2, Caspase-3, β-actin, GAPDH and Horseradish peroxidase-conjugated Goat Anti-Mouse antibody were obtained from Proteintech Group (Rosemont, IL, United States). MDA assay Kit (S0131), GSH and GSSG Assay Kit (S0053), Reactive Oxygen Species Assay Kit (S0033S) and Horseradish peroxidase-conjugated Goat Anti-Rabbit antibody were provided by Beyotime Biotech (Nantong, China). p44/42 MAPK (ERK1/2) (4695S), Phospho-p44/42 MAPK (p-ERK1/2) (8544S), NF-κB p65 (p65) (8242S) and Phospho-NF-κB p65 (P-p65) (4025S) were obtained from Cell Signaling Technology (Beverly, MA, United States). NGAL antibody is provided by Abcam (Cambridge, MA, United States). Dimethyl sulfoxide (DMSO) was provided from Sigma-Aldrich Chemicals (St. Louis, MO, United States). DAB (SA-HRP) TUNEL Cell Apoptosis Detection Kit (G1507) was from Servicebio (Wuhan, China). All other chemicals used in the study were commercially available and of analytical grade.

Wang H., Xia W., Long G., Pei Z., Li Y., Wu M., Wang Q., Zhang Y., Jia Z, & Chen H. (2020). Isoquercitrin Ameliorates Cisplatin-Induced Nephrotoxicity Via the Inhibition of Apoptosis, Inﬂammation, and Oxidative Stress. Frontiers in Pharmacology, 11, 599416.

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