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3 protocols using rabbit anti h3k14ac

1

Antibody Validation for Toxoplasma Research

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The following primary antibodies were used in the immunofluorescence, immunoblotting and/or ChIP assays: rabbit home-made anti-BCLA, rabbit anti-TgHDAC3 (RRID: AB_2713903), rabbit anti-H4K31ac (RRID: AB_2811024), rabbit anti-H4K31me1 (RRID: AB_2811025), rabbit anti-TgGAP45 (gift from Pr. Dominique Soldati, University of Geneva), rat anti-CC2 (gift from W. Bohne and U. Gross), mouse anti-TgBAG1, mouse anti-G1/19 (gift from Pr. Gereon Schares, Friedrich-Loeffler-Institut), mouse anti-HA tag (Roche, RRID: AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology, RRID: AB_1549585), rabbit anti-mCherry (Cell Signaling Technology, RRID: AB_2799246), rabbit anti-FLAG (Cell Signaling Technology, RRID: AB_2798687), rabbit anti-H3K14ac (Diagenode, RRID:AB_2713906), H3K9me3 (Millipore, RRID:AB_916348), H3K4me3 (Diagenode, RRID:AB_2616052), rabbit Anti-acetyl-Histone H4, pan (Lys 5,8,12) (Millipore, RRID:AB_310270). Immunofluorescence secondary antibodies were coupled with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific). Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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2

Antibody Validation for Toxoplasma Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used in the immunofluorescence, immunoblotting and/or ChIP assays: rabbit home-made anti-BCLA, rabbit anti-TgHDAC3 (RRID: AB_2713903), rabbit anti-H4K31ac (RRID: AB_2811024), rabbit anti-H4K31me1 (RRID: AB_2811025), rabbit anti-TgGAP45 (gift from Pr. Dominique Soldati, University of Geneva), rat anti-CC2 (gift from W. Bohne and U. Gross), mouse anti-TgBAG1, mouse anti-G1/19 (gift from Pr. Gereon Schares, Friedrich-Loeffler-Institut), mouse anti-HA tag (Roche, RRID: AB_2314622), rabbit anti-HA Tag (Cell Signaling Technology, RRID: AB_1549585), rabbit anti-mCherry (Cell Signaling Technology, RRID: AB_2799246), rabbit anti-FLAG (Cell Signaling Technology, RRID: AB_2798687), rabbit anti-H3K14ac (Diagenode, RRID:AB_2713906), H3K9me3 (Millipore, RRID:AB_916348), H3K4me3 (Diagenode, RRID:AB_2616052), rabbit Anti-acetyl-Histone H4, pan (Lys 5,8,12) (Millipore, RRID:AB_310270). Immunofluorescence secondary antibodies were coupled with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific). Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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3

Histone Modifications and HDAC3 Detection

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Primary antibodies: rabbit homemade anti-TgHDAC3 described in Bougdour et al. (2009 (link)); RRID:AB_2713903), mouse anti-HA (Roche, RRID:AB_2314622), rabbit anti-H4K8ac (Millipore, RRID:AB_310524), rabbit anti-H4K12ac (Millipore, RRID:AB_11215637), rabbit anti-H3K4ac (Diagenode, RRID:AB_2713904), rabbit anti-H3K9ac (Diagenode RRID:AB_2713905), rabbit anti-H3K14ac (Diagenode, RRID:AB_2713906), rabbit anti-H3K18ac (Diagenode, RRID:AB_2713907), rabbit anti-H3K14ac (Millipore, RRID:AB_1977241) and mouse anti-H3K27ac (Diagenode, RRID:AB_2713908), anti-H4K20me3 (Diagenode, RRID:AB_2713909), anti-H3K9me3 (Millipore, RRID:AB_916348), anti-H3K4me1 (Diagenode, RRID:AB_2637078) and anti-H3K4me3 (Diagenode, RRID:AB_2616052). Western blot secondary antibodies were conjugated to alkaline phosphatase (Promega), whereas immunofluorescence secondary antibodies were coupled with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific). We also raised homemade H4K31acetylation- and H4K31monomethylation-specific antibodies in rabbit against linear peptides corresponding to amino acid residues 23–35 of histone H4 and carrying modified residue K31: C-DNIQGITKme1PAIR; C-DNIQGITKacPAIR and C-RDNIQGITKacPAIR. They were produced by Eurogentec and used for immunofluorescence, immunoblotting and chromatin immunoprecipitation.
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