Winmdi version 2

Manufactured by BD

Sourced in United States

WinMDI version 2.9 is a software application designed for the analysis and visualization of flow cytometry data. It provides a user-friendly interface for displaying and interpreting cytometry measurements.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions) Facscalibur cytometer, by BD (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Facsdiva software, by BD (1 mentions)

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WinMDI 2.9 software (29 citations) WinMDI (3 citations)

4 protocols using winmdi version 2

Annexin V Apoptosis Assay in Liver Cells

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Apoptosis levels were determined by Annexin V fluorescence assay. Hep3B and L02 cells were seeded in 6‐well plates at 2 × 10⁵ cells/well and following 0, 6‐, and 12‐hr incubation with 10 mg/ml HP. Levels of apoptosis were measured using the Annexin V‐FITC and PI detection Kit (Solarbio life sciences); signal was detected by fluorescence microscopy (EVOS^®xl core cell culture microscope, Advanced Microscopy Group, Paisley) and flow cytometry (FACSCalibur, BD Biosciences); data were analyzed using WinMDI version 2.9 (BD Biosciences).

Wei L., Dong Y., Sun Y., Mei X., Ma X., Shi J., Yang Q., Ji Y., Zhang Z., Sun H., Sun X, & Song S. (2021). Anticancer property of Hemp Bioactive Peptides in Hep3B liver cancer cells through Akt/GSK3β/β‐catenin signaling pathway. Food Science & Nutrition, 9(4), 1833-1841.

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Annexin V-FITC Apoptosis Assay

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The cells were first seeded in six-well culture plates (Orange Scientific, Braine-l’Alleud, Belgium). After treatment with SFN for 4 h, the cells were harvested and centrifuged. The cell pellet was then resuspended and incubated with 1× annexin-binding buffer [5 μL of annexin V-FITC (BD Pharmingen, Franklin Lakes, NJ, USA) and 1 μL of 100 μg/mL propidium iodide (PI) working solution] for 15 min at room temperature. After incubation, the stained cells were detected by a FACSCalibur flow cytometer (BD Pharmingen) and analyzed using WinMDI version 2.9 (BD Pharmingen).

Hung C.M., Tsai T.H., Lee K.T, & Hsu Y.C. (2023). Sulforaphane-Induced Cell Mitotic Delay and Inhibited Cell Proliferation via Regulating CDK5R1 Upregulation in Breast Cancer Cell Lines. Biomedicines, 11(4), 996.

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Cell Cycle Analysis with Propidium Iodide

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Cell cycle analysis was carried out using the fluorescent intercalating agent propidium iodide (PI) method. Briefly, HT-29 cells were seeded on 6-cm plates and treated with auransterol (0–50 μM) or paclitaxel (0.01 μM) for 24 h. Cells washed with phosphate-buffered saline (PBS) were treated with trypsin, and re-suspended in cold water. Then, cells were fixed with ethanol (70%), washed, and re-suspended in PBS. The RNAse (20 μg/mL) was then added to cells and incubated for 1 h to add PI (1.0 μg/mL) prior reading. The analysis was made using a BD FACS Calibur cytometer at an excitation wavelength of 488 nm and an emission wavelength of 617 nm. The cellular DNA profile was analyzed using the software WinMDI version 2.8 (BD, USA) [11 (link)].

Anaya-Eugenio G.D., Tan C.Y., Rakotondraibe L.H, & Carcache de Blanco E. (2020). Tumor suppressor p53 independent apoptosis in HT-29 cells by auransterol from Penicillium aurantiacobrunneum. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 127, 110124.

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Lipid Droplet Quantification in Lung Cells

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Uninfected and H37Rv-infected lungs were finely chopped and digested in 5 ml RPMI with 5% FBS containing 150 U/ml Collagenase IV (HiMedia) and 50 U/ml DNaseI (Thermo Fisher Scientific) at 37°C in shaking condition for 1 h. The cell suspension was passed through 40 μm cell strainer and pelleted at 1500 rpm for 10 min. The cells were resuspended in RBC lysis buffer. Post lysis, the cells were washed with PBS. Obtained cell suspension was fixed using 3.6% formaldehyde solution for 30 min. After 3 washes with PBS, cells were resuspended in PBS containing 2 μg/ml BODIPY 493/503 and incubated for 30 min at room temperature in dark under mild rocking condition. After thorough washes with PBS, cells were analysed by flow cytometry wherein 1 lakh events were recorded for each sample acquired in BD FACSCanto II. The data was analyzed using FACSDiva software (BD Biosciences) and WinMDI Version 2.8.

Holla S., Prakhar P., Singh V., Karnam A., Mukherjee T., Mahadik K., Parikh P., Singh A., Rajmani R.S., Ramachandra S.G, & Balaji K.N. (2016). MUSASHI-Mediated Expression of JMJD3, a H3K27me3 Demethylase, Is Involved in Foamy Macrophage Generation during Mycobacterial Infection. PLoS Pathogens, 12(8), e1005814.

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