The largest database of trusted experimental protocols

Sab4301803

Manufactured by Santa Cruz Biotechnology

The SAB4301803 is an analytical instrument designed for performing various laboratory procedures. It is a high-precision tool that can be utilized for a range of applications within the scientific research and development field. The core function of this product is to provide reliable and accurate data acquisition and analysis capabilities to support the needs of modern laboratory settings.

Automatically generated - may contain errors

2 protocols using sab4301803

1

Protein Detection Using Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used the following reagents to detect proteins: monoclonal anti-G3BP (Sigma-Aldrich WH0010146M1), polyclonal anti-TIA1 produced in rabbit (Sigma-Aldrich SAB4301803), anti-GAPDH (sc-47724, Santa Cruz Biotechnology), eIF2α (9722, Cell Signaling Technology), Phospho-eIF2α (Ser51; 9721, Cell Signaling Technology).
Secondary antibodies: anti-Rabbit IgG Cy3-conjugated (Sigma-Aldrich C2306), anti-Mouse IgG Cy3-conjugated (Sigma-Aldrich C2181), anti-rabbit IgG Cy5 conjugated (Invitrogen A10523), Goat Anti-Mouse IgG H&L (20 nm Gold) preadsorbed (ab27242).
+ Open protocol
+ Expand
2

Protein Detection Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used the following reagents to detect proteins: monoclonal anti-G3BP (Sigma-Aldrich WH0010146M1), polyclonal anti-TIA1 produced in rabbit (Sigma-Aldrich SAB4301803), anti-GAPDH (sc-47724, Santa Cruz Biotechnology), anti-PPARA (sc-398394, Santa Cruz Biotechnology), anti-PPARG (sc-7273X, Santa Cruz Biotechnology), anti-PPARD (sc-74517, Santa Cruz Biotechnology), antiphospho-4EBP (Ser65, sc-293124), and anti-4EBP (sc-9977, Santa Cruz Biotechnology).
Secondary antibodies for immunofluorescence: anti-rabbit IgG Cy3-conjugated (Sigma-Aldrich C2306), anti-mouse IgG Cy3 conjugated (Sigma-Aldrich C2181), and anti-rabbit IgG Cy5 conjugated (Invitrogen A10523).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!