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Aktpos473 4

Manufactured by Cell Signaling Technology
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The AKTpoS473/4 is a laboratory reagent that detects phosphorylation of the AKT protein at serine residues 473 and 474. It is used in research applications to measure AKT activity and signaling pathways.

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Lab products found in correlation

α tubulin sc 5286, by Santa Cruz Biotechnology (2 mentions) Pras40, by Cell Signaling Technology (2 mentions) Pi3k p110α, by Cell Signaling Technology (2 mentions) Gapdh sc 32233, by Santa Cruz Biotechnology (2 mentions) Aktpos473, by Cell Signaling Technology (2 mentions) Akt2pos474, by Cell Signaling Technology (2 mentions) Aktpot308, by Cell Signaling Technology (2 mentions) Smad4 ab 40759, by Abcam (2 mentions) β actin sc 47778, by Santa Cruz Biotechnology (2 mentions) Rxxs t, by Cell Signaling Technology (1 mentions)

2 protocols using aktpos473 4

1

Western Blot Analysis of Protein Signaling

Cited 1 time
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Tissue was homogenized, or cultured cells washed, and then lysed in complete RIPA buffer as described previously [50 (link)]. Primary antibodies (Ab) used: AR (sc-816), PI3K p110β (sc-376641), β-actin (sc-47778), GAPDH (sc-32233), and α-tubulin (sc-5286) from Santa Cruz Biotechnology (Santa Cruz, CA); AKT1 (#2938), AKT2 (#3063), AKT3 (#8018), AKT3 (#14982), AKTpoS473 (#9018), AKTpoS473/4 (#9271), AKT2poS474 (#8599), AKTpoT308 (#4056S), phospho-AKT substrate RXXS*/T* (#9614S), PRAS40 (#2691S), PRAS40poT346 (#2997T), PTEN (#9552), and PI3K p110α (#4249) from Cell Signaling Technologies (Beverly, MA); SMAD4 (ab-40759) from Abcam (Cambridge, MA). Between 15 and 35 μg of total protein per sample was separated by SDS-PAGE for IB.
Miller K., Degan S., Wang Y., Cohen J., Ku S.Y., Goodrich D, & Gelman I. (2023). PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression. Research Square.
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2

Protein Extraction and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was homogenized, or cultured cells washed, and then lysed in complete RIPA buffer as described previously [50 (link)]. Primary antibodies (Ab) used: AR (sc-816), PI3K p110β (sc-376641), β-actin (sc-47778), GAPDH (sc-32233), and α-tubulin (sc-5286) from Santa Cruz Biotechnology (Santa Cruz, CA); AKT1 (#2938), AKT2 (#3063), AKT3 (#8018), AKT3 (#14982), AKTpoS473 (#9018), AKTpoS473/4 (#9271), AKT2poS474 (#8599), AKTpoT308 (#4056S), phospho-AKT substrate RXXS*/T* (#9614S), PRAS40 (#2691S), PRAS40poT46 (#2997T), PTEN (#9552), and PI3K p110α (#4249) from Cell Signaling Technologies (Beverly, MA); SMAD4 (ab-40759) from Abcam (Cambridge, MA); SSeCKS/Akap12 [12 (link)]. Between 15 and 35 µg of total protein per sample was separated by SDS-PAGE for IB.
Miller K.A., Degan S., Wang Y., Cohen J., Ku S.Y., Goodrich D.W, & Gelman I.H. (2023). PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression. Oncogene, 43(1), 22-34.
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