All images were obtained with a Leica SP5 Spectral Confocal Microscope or Zeiss LSM880 Airy Scan Fast Confocal System with 10× or 20× objectives. Multifield large images were taken with 10% overlap and stitched with the Leica Application Suite or Zeiss Zen Microscope Software. Z-stacks were obtained using 1 μm or 2 μm step sizes. Images were processed with ImageJ. Figures and anatomical drawings were made using Adobe Illustrator 2022.
Sp5 spectral confocal microscope
Manufactured by Zeiss
The SP5 Spectral Confocal Microscope is a high-performance imaging system designed for advanced microscopy applications. It features a spectral detection system that enables the simultaneous acquisition of multiple fluorescence signals within a single image. The microscope provides high resolution, sensitivity, and flexibility for a wide range of sample types and research areas.
Automatically generated - may contain errors
2 protocols using sp5 spectral confocal microscope
1
Confocal Microscopy Imaging Protocol
2
Confocal Microscopy Imaging Protocol
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All images were obtained with a Leica SP5 Spectral Confocal Microscope or Zeiss LSM880 Airy Scan Fast Confocal System with 10× or 20× objectives. Multifield large images were taken with 10% overlap and stitched with the Leica Application Suite or Zeiss Zen Microscope Software. Z‐stacks were obtained using 1 μm or 2 μm step sizes. Images were processed with ImageJ. Figures and anatomical drawings were made using Adobe Illustrator 2022.
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