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6 protocols using polyethyleneimine pei

1

Polysulfone Membrane for Dye Removal

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The polysulfone (PSF) ultrafiltration (UF) substrate, with a MWCO of 30 kDa, were provided by Pureach Tech Ltd (Beijing, China). Polyethyleneimine (PEI, MW: 70 000 Da, 50 wt%), trimesoyl chloride (TMC, 98.0%), and isophthaloyl chloride (IPC, 98.0%) were purchased from Shanghai Macklin Biochemical Co., Ltd., China. n-Hexane and all inorganic electrolytes with AR grade, including ZnCl2, MgCl2, CaCl2, CuCl2, MgSO4, NaCl, and Na2SO4, were purchased from SINOPHARM, China. The dyes, including cationic red X-GTL, rhodamine B, cationic gold yellow X-GL, and reactive brilliant blue KN-R, were obtained from Tianjin Longshengxiang Science and Technology Ltd., China. Fig. 1 shows the molecular structures of different dyes. Milli-Q water was used for the preparation and the permeation tests. All chemicals were used without further purification.
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2

TEOS-PEI Nanoparticles for Curcumin Delivery

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Tetraethylorthosilicate (TEOS), and polyethyleneimine (PEI) were obtained from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Ammonia water (NH3·H2O, 14.84 M), triethanolamine, 3-triethoxysilylpropylamine (APTE), anhydrous sodium carbonate (Na2CO3), curcumin (Cur), glutaraldehyde (GA), and phosphate buffered saline (PBS), were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dialysis membrane and cetyltrimethylammonium bromide (CTAB) were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). Tryptone, yeast extract was obtained from Oxoid Ltd. (Basingstoke, New Hampshire, UK). Agar was obtained from Beijing Jin Ming Biotechnology Co., Ltd. (Beijing, China) Trypticase Soy Broth was obtained from Qingdao Hope Bio-Technology Co., Ltd. (Qindao, China) S. aureus (ATCC29213) is a laboratory collection standard strain. All other chemicals in the experiment are analytical reagents and can be used without further purification.
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3

Diclofenac Sodium Ocular Delivery System

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Diclofenac sodium (DS) was acquired from the Wuhan Dongkangyuan Technology Co., Ltd. (Wuhan, Hubei, China). Polyethyleneimine (PEI, Mw = 10 kDa) was purchased from Macklin® (Shanghai, China). Carboxymethyl chitosan (CMC, Mw = 197.17 kDa) was obtained from the Eisie Chemical Co., Ltd. (Jiaxing, Zhejiang, China). Hyaluronic acid (HA, MW < 10 kDa) was purchased from Freda (Jinan, Shandong, China). Poloxamer 407 (F407) and Poloxamer 188 (F68) were acquired from BASF (Ludwigshafen, Rhineland-palatinate, Germany). Hydroxypropyl methylcellulose E50 (HPMC E50) was a gift from the Anhui Shanhe Pharmaceutical Excipients Co., Ltd. (Huainan, Anhui, China). Other reagents involved in this work were all of analytical grade.
New Zealand albino rabbits (weighing 2.0–2.5 kg), having eyes without injury, were obtained from the Shenyang Pharmaceutical University Animal Center (Shenyang, Liaoning, China). All animal procedures in this study were conducted according to the principles of laboratory animal care and approved by the Animal Ethical Committee of Shenyang Pharmaceutical University.
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4

Synthesis of Ti3AlC2 Composite Materials

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Ti3AlC2 powder and silver nitrate (AgNO3) were provided by Macklin Co., Ltd. Polyethyleneimine (PEI, Mw = 10 000 g mol−1), lithium fluoride (LiF), hydrochloric acid (HCl), acetone, and ethyl alcohol were purchased from Sinopharm Chemical Reagent Co., Ltd. Ethylene glycol (EG), polyvinylpyrrolidone (PVP), and cellulose were obtained from Sigma-Aldrich. All the commercial chemicals were used as received.
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5

Synthesis of SiO2 Nanoparticle-Coated PPS Filters

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Silicon dioxide (SiO2) nanoparticles and polytetrafluoroethylene (PTFE, 60 wt.% dispersion) were purchased from Aladdin (Shanghai, China). Polyethyleneimine (PEI, typical MW = 70,000, 50 wt.% aqueous solution) was bought from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Triethanolamine, sodium dodecylbenzene sulfonate, silane coupling agent (KH-550) and ethanol were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Polyphenylene sulfide (PPS, grammage: 550 g m−2) and PTFE membrane-coated PPS filter medium (grammage: 550 g m−2) were purchased from Liaoning Xinhongyuan Environmental Protection Material Co. Ltd. (Yingkou, Liaoning, China). All the chemicals were guaranteed reagents and used without further purification. The water used was purified by a Milli-Q Advantage A10 system (Millipore, Bedford, MA, USA) with a resistivity higher than 18.2 MΩ cm−1.
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6

Milk-based Luminescent Nanomaterials Synthesis

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Pure milk was purchased from Liaocheng University campus supermarket. The metal salts were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). They included Tb(CH 3 COO) 3 , Nd(CH 3 COO) 3 , Pr(CH 3 COO) 3 , Er(CH 3 COO) 3 , NaCl, and NaOH. Polyethyleneimine (PEI, MW=10000, 99%) was ordered from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Acetaldehyde (AA), dimethylformamide (DMF), acetone, methanol, chloroform, acetonitrile, ether, ethyl acetate (EA), formaldehyde (FA), propionaldehyde (PA), and butyraldehyde (BA) were provided by Sinopharm Chemical Rea-gent Co., Ltd. (Shanghai, China). All chemicals used in relevant experiments were analytical grade and without further puri cation. Deionized water supplied by the Milli-Q water Puri ed system was used in all experiments.
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