Recombinant human IL1B (200-01B) and IL-1R antagonist (IL1-RA, 200-01R) were purchased from Peprotech (Rocky Hill, NJ). p38 MAPK (8690), phospho-p38 MAPK (Thr180/Tyr182; 4511), p42/44 MAPK (9107S), phospho-p42/44 MAPK (Thr202/Tyr204; 9101) antibodies were purchased from Cell Signaling Technology (Beverly, MA). MAPK inhibitors SP600125, SB202190 and SB203580 were purchased from Sigma Aldrich (St Louis, MO), U0126 and BAY869766 were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA).
The human breast cancer lines: MDA-MB-231, MDA-MB-436, BT549, SKBR3, ZR75-1, HCC1954 were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA), 2 mM L-glutamine, and 50 μg/mL gentamicin (Life Technologies, Carlsbad, CA). THP-1 monocyte cells were cultured in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, and 1% antibiotic/antimycotic solution (15240062, Life Technologies). All cell lines were recently acquired from the ATCC (Manassas, VA). Cell lines were incubated in a humidified atmosphere of 5% CO2 at 37 °C.
The human breast cancer lines: MDA-MB-231, MDA-MB-436, BT549, SKBR3, ZR75-1, HCC1954 were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA), 2 mM L-glutamine, and 50 μg/mL gentamicin (Life Technologies, Carlsbad, CA). THP-1 monocyte cells were cultured in RPMI 1640 supplemented with 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, and 1% antibiotic/antimycotic solution (15240062, Life Technologies). All cell lines were recently acquired from the ATCC (Manassas, VA). Cell lines were incubated in a humidified atmosphere of 5% CO2 at 37 °C.