2 7 dichlorodihydrofluorescein diacetate h2dcfda d399

All media and supplements for cell culture were supplied by Hyclone™ (GE Healthcare Life Sciences, Logan, UT, USA). Dimethyl sulfoxide (DMSO), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), RIPA buffer, protease [glutathione (GSH)] and phosphatase (ascorbic acid) inhibitors, Hoechst 33342 dye and propidium iodide (PI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA; D399) and rhodamine 123 were purchased from Molecular Probes (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Rabbit anti-GAPDH polyclonal antibody (cat. no. ab9485; dilution 1:2,000) was supplied by Abcam (Cambridge, UK); mouse anti-poly ADP-ribose polymerase (PARP) polyclonal antibody (cat. no. AM30; dilution 1:200) was obtained from Merck Millipore (Darmstadt, Germany); and goat anti-epidermal growth factor receptor (EGFR) polyclonal antibody (cat. no. sc-03-G; dilution 1:200) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

The HN-3 cell line was obtained from the Asan Medical Center (Seoul, South Korea) (27 (link)). Hoechst 33342 dye, MTT and propidium iodide (PI) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). CBDCA was purchased from Selleck Chemicals (Houston, TX, USA). Antibodies against cleaved caspase-3 (rabbit, cat. no. AB3623), cleaved poly(ADP-ribose) polymerase (PARP; rabbit, cat. AB3620) were purchased from Merck KGaA (Darmstadt, Germany); goat anti-epidermal growth factor receptor (EGFR; cat. no. sc-03-G; dilution 1:200) and phosphorylated c-Jun (p-c-Jun; cat. no. sc-99) were from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); rabbit anti-GAPDH polyclonal antibody (cat. no. ab9485; dilution 1:2,000) was supplied by Abcam (Cambridge, UK). 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA; D399) was purchased from Molecular Probes (Thermo Fisher Scientific Inc., Waltham, MA, USA).