Fsl 1 pam2cgdpkhpksf

Manufactured by InvivoGen

Sourced in United States

FSL-1 (Pam2CGDPKHPKSF) is a synthetic lipoprotein that activates Toll-like receptor 2 (TLR2). It consists of a lipid moiety (Pam2C) and a peptide sequence (GDPKHPKSF). The core function of FSL-1 is to serve as a TLR2 agonist, which can be used in research applications involving the study of TLR2-mediated immune responses.

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Lab products found in correlation

Human keratinocyte growth supplement, by Thermo Fisher Scientific (1 mentions) Collagen type 4, by Merck Group (1 mentions) Epilife growth medium, by Thermo Fisher Scientific (1 mentions) Ps 1145 dihydrochloride, by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Muramyl dipeptide mdp, by InvivoGen (1 mentions) Hek blue selection, by InvivoGen (1 mentions) Quanti blue, by InvivoGen (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Ibuprofen, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Blasticidin, by InvivoGen (1 mentions) Cell proliferation reagent wst 1, by Roche (1 mentions) Mtt assay, by Thermo Fisher Scientific (1 mentions) Pam3csk4 p3c, by InvivoGen (1 mentions)

3 protocols using fsl 1 pam2cgdpkhpksf

Cell Culture and Treatment Protocols

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HEKs cells (catalog no. C‐005‐5C; Gibco) were cultured in EpiLife growth medium (catalog no. MEPI500CA; Gibco) supplemented with human keratinocyte growth supplement (catalog no. S‐001‐K; Gibco). Cells were seeded on collagen type IV (catalog no. C5533; Sigma‐Aldrich) precoated plates. Human SCC cells (A431; ATCC, VA) were cultured in Dulbecco's modified Eagle's medium (catalog no.11995‐065; Gibco) supplemented with 10% fetal bovine serum (catalog no. 098105; Multicel). PS‐1145 dihydrochloride (catalog no. P6624; Sigma‐Aldrich) was dissolved in dimethyl sulfoxide (DMSO; catalog no. D5879; Sigma‐Aldrich) and diluted at least 1000‐fold in culture medium before treatment. Tumor necrosis factor (TNF; catalog no.210‐TA‐020; R&D Systems) was dissolved in phosphate‐buffered saline (PBS) containing 0.1% bovine serum albumin (BSA; catalog no. A3095; Sigma‐Aldrich). FSL1 (Pam2CGDPKHPKSF, catalog no. tlr‐fsl; InvivoGen).

Altonsy M.O., Kurwa H.A., Lauzon G.J., Amrein M., Gerber A.N., Almishri W, & Mydlarski P.R. (2020). Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor‐κB inflammatory signaling pathway in human skin cells. Immunity, Inflammation and Disease, 8(1), 62-79.

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Characterization of HEK-Blue Cell Lines

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HEK-Blue™-2, HEK-Blue™-4, and nucleotide oligomerization domain (NOD)2-Wild Type (NOD2-WT) embryonic kidney cell lines were selected due to their accessibility and relatively high expression of matrix metalloproteinase (MMP)-1, -2, and -9. These were obtained from the Auckland Cancer Society Research Centre (Auckland NZ). Dulbecco’s Modified Eagle’s Medium (DMEM), an antibiotic mixture (penicillin, streptomycin, L-glutamine), and fetal calf serum (FCS) were obtained from Life Technologies Corp (Carlsbad CA, USA). Phorbol 12-myristate 13-acetate (PMA) and ibuprofen were purchased from Sigma-Aldrich Corp (St Louis, MO, USA). Lipopolysaccharide (LPS), Pam3CysSerLys4 (Pam3CSK4), and FSL-1 (Pam2CGDPKHPKSF, a synthetic diacylated lipoprotein), Muramyl dipeptide (MDP), Blasticidin, Zeocin™, HEK-Blue™ Selection, and QUANTI-Blue™ were from InvivoGen (San Diego, CA, USA). Cell Proliferation Reagent WST-1 was obtained from Roche Applied Science (Penzberg, Germany).

Tomblin V., Ferguson L.R., Han D.Y., Murray P, & Schlothauer R. (2014). Potential pathway of anti-inflammatory effect by New Zealand honeys. International Journal of General Medicine, 7, 149-158.

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TLR Ligands Viability Assay

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GC cell lines were grown in 12-well plates in triplicate (1 × 10 5 cells/well). After serum-free starvation overnight, cells were treated with phosphate-buffered saline (PBS control), Pam3CSK4 (P3C, InvivoGen) 10 µg/ml, Lipopolysaccharides from Escherichia coli O111:B4 (LPS, Sigma) 10 ng/ml, FSL-1 (Pam2CGDPKHPKSF, InvivoGen) 1 µg/ml, Heat Killed Listeria monocytogenes (HKLM, InvivoGen) 10 7 -10 8 cells/ml, ODN2006 (ODN7909, Stimulatory CpG ODN, Class B, InvivoGen) 2 µM for 24 h. Cell viability assay in response to TLR ligands for cell number normalization was also conducted using the MTT assay (Invitrogen) in 96-well plate following the manufacturer's instructions.

li y., Ji C., liu y., Gu Q., Jenkins B.J., li j., & Yu L. (2020). Activation of Toll-like receptor 2 via specific agonists promotes OXPHOS and glycolysis in gastric cancer cells rather than other Toll-like receptors.

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