Unless stated otherwise, chemicals and reagents were from Sigma (St
Louis, MO, USA). The antibody for cyclin D1 was from Cell Signaling Technology
Inc. (Beverly, MA, USA), for ALDH1A1 from Abcam (Cambridge, MA, USA), for
β-catenin from ECM Biosciences (Versailles, KY, USA), and for GAPDH from
Biodesign International (Saco, ME, USA). Secondary HRP-conjugated antibodies
were from Amersham Biosciences (San Francisco, CA, USA) and Santa Cruz
Biotechnology Inc (Santa Cruz, CA, USA). The Aldefluor kit assay was from
StemCell Technologies (Vancouver, BC Canada). The ALDH1A1 inhibitor (A37) was
from ChemDiv (San Diego, CA, USA), having >95% purity.
Louis, MO, USA). The antibody for cyclin D1 was from Cell Signaling Technology
Inc. (Beverly, MA, USA), for ALDH1A1 from Abcam (Cambridge, MA, USA), for
β-catenin from ECM Biosciences (Versailles, KY, USA), and for GAPDH from
Biodesign International (Saco, ME, USA). Secondary HRP-conjugated antibodies
were from Amersham Biosciences (San Francisco, CA, USA) and Santa Cruz
Biotechnology Inc (Santa Cruz, CA, USA). The Aldefluor kit assay was from
StemCell Technologies (Vancouver, BC Canada). The ALDH1A1 inhibitor (A37) was
from ChemDiv (San Diego, CA, USA), having >95% purity.