Total RNA in cells and tissue samples was extracted by using TRIZOL reagent (Thermo). Superscript III reverse transcriptase (Thermo) was used to synthesize cDNA templates. RT-qPCR was carried out using a SYBR ExScript qPCR kit (Takara, Dalian, China) under the following conditions: pre-denaturation at 95°C for 2 min, degeneration at 94°C for 15 s, annealing at 55°C for 25 s, and extension at 72°C for 15 s with 35 cycles.
GAPDH was used as the internal reference for GATA2 and LINC00891, and
U6 was used as the internal reference for miR-128-3p. The relative expression of the candidate genes was calculated according to the 2
–ΔΔCT method. Primer sequences are as follows:
U6 forward 5′-CTCGCTTCGGCAGCACA-3′, and reverse 5′-AACGCTTCACGAATTTGCGT-3′;
GAPDH forward 5′-AAGAAGGTGGCCTGCGCAGGC-3′, and reverse 5′-TCCACCACCCGGTTGTTGCGC-3′;
miR-128-3p forward 5′-GCCGGCGCCCGAGCTCTGGCTC-3′, and reverse 5′-TCACAGTGAACCGGTCTCTTT-3′;
LINC00891 forward 5′-AAGGCACCTGACATCACCTG-3′, and reverse 5′-GGGTCATGAGACACCTGTGG-3′;
GAPDH was used as the internal reference for GATA2 and LINC00891, and
U6 was used as the internal reference for miR-128-3p. The relative expression of the candidate genes was calculated according to the 2
–ΔΔCT method. Primer sequences are as follows:
U6 forward 5′-CTCGCTTCGGCAGCACA-3′, and reverse 5′-AACGCTTCACGAATTTGCGT-3′;
GAPDH forward 5′-AAGAAGGTGGCCTGCGCAGGC-3′, and reverse 5′-TCCACCACCCGGTTGTTGCGC-3′;
miR-128-3p forward 5′-GCCGGCGCCCGAGCTCTGGCTC-3′, and reverse 5′-TCACAGTGAACCGGTCTCTTT-3′;
LINC00891 forward 5′-AAGGCACCTGACATCACCTG-3′, and reverse 5′-GGGTCATGAGACACCTGTGG-3′;