Tissue sections were incubated with primary antibody to Drd5, F4/80, Inos, and Arg1 sections at 4 °C overnight, and then incubated with secondary antibody as indicated. The nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) (sigma). Slides were dried and mounted using ProLong Antifade mounting medium (Beyotime Biotechnology). At last, slides were visualized using a Nikon 50i fluorescent microscope. The number of macrophages from three images that were randomly selected from each tissue section were quantified using Image pro plus. The cell number shown in Fig. 6A (y) is derived from this formula. y = N/S (y, number of cells per mm2; N, the total number of cells per tissue section; S, area).
Prolong antifade mounting medium
Manufactured by Beyotime
ProLong Antifade mounting medium is a specialized reagent designed for use in fluorescence microscopy. It is formulated to reduce photobleaching of fluorescent dyes, preserving the signal intensity and enabling longer observation of samples.
Automatically generated - may contain errors
4 protocols using prolong antifade mounting medium
1
Quantitative Analysis of Macrophage Markers
2
Multiparametric Immunofluorescence Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections were incubated at 4°C overnight with primary antibodies to IBA1, GFAP, MBP, and CD4. Slides were then incubated with indicated secondary antibodies. The nuclei were counterstained with DAPI (Sigma-Aldrich). Slides were dried and mounted using ProLong Antifade mounting medium (Beyotime Biotechnology). Slides were visualized using a Nikon 50i fluorescent microscope.
3
Immunofluorescence Analysis of IBA1, APOE, and CD11b
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections were incubated at 4°C overnight with primary antibody to IBA1, APOE, and CD11b. Slides were then incubated with indicated secondary antibodies. The nuclei were counterstained with DAPI (Sigma-Aldrich). Slides were dried and mounted using ProLong Antifade mounting medium (Beyotime Biotechnology). Slides were visualized by a Nikon 50i fluorescent microscope.
4
Immunofluorescence Staining of MUC2, GSDMD, E-cadherin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue sections were incubated at 4°C overnight with primary antibody to MUC2, GSDMD, and E-cadherin. Slides were then incubated with indicated secondary antibodies. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich). Slides were dried and mounted using ProLong Antifade mounting medium (Beyotime Biotechnology). Slides were visualized using a Nikon 50i fluorescence microscope or a Zeiss LSM 700 META laser scanning confocal microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!