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4 mup alkaline phosphatase substrate

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4-MUP alkaline phosphatase substrate is a fluorogenic substrate used for the detection and quantification of alkaline phosphatase enzyme activity in biological samples. It generates a fluorescent product upon enzymatic cleavage, allowing for sensitive and specific measurement of alkaline phosphatase levels.

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Lab products found in correlation

Amplex ultra red soluble hrp fluorophore, by Thermo Fisher Scientific (2 mentions) Rpepo rpe23 16086 1 ap, by Proteintech (2 mentions) Amplex ultrared soluble fluorophore hrp substrate, by Thermo Fisher Scientific (2 mentions) Rpl23 16086 1 ap, by Proteintech (2 mentions) Ab64193, by Abcam (1 mentions) Ab89780, by Abcam (1 mentions) Ab10148, by Abcam (1 mentions) Ab109390, by Abcam (1 mentions) Ab7260, by Abcam (1 mentions)

4 protocols using 4 mup alkaline phosphatase substrate

1

Multiparametric Immunohistochemistry of Neurodegenerative Markers

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Antibodies to tau (ab64193), S396 (ab 109390) and T205 (ab4841) phospho-tau, ubiquitin (UBI-1 ab7780), myelin-associated glycoprotein (MAG-1; ab89780), glial fibrillary acidic protein (GFAP; ab7260), 3-nitrotyrosine (NTyR; ab61392), Hu neuronal marker (ab78467), Complex IV (abl4744), ATP synthase (abl81243), 8-hydroxyguanosine (8-OHdG; abl0802), common leukocyte antigen (CD45; abl0558), and 4-hydroxynonenal (HNE; ab46545), and the EDH cytotoxicity assay were purchased from Abeam (Cambridge, MA). Rabbit polyclonal antibody to RPEPO (RPE23 16086-1-AP) was purchased from Proteintech Inc (Chicago, IL). Amplex Ultra Red soluble HRP fluorophore and 4-MUP alkaline phosphatase substrate were purchased from Invitrogen (Carlsbad, CA). The L165, 041 PPAR-δ agonist and F-L-Leu PPAR-γ agonist were purchased from Cal Biochem (San Diego, CA).
Reich D., Gallucci G., Tong M, & de la Monte S. (2018). Therapeutic Advantages of Dual Targeting of PPAR-δ and PPAR-γ in an Experimental Model of Sporadic Alzheimer’s Disease. Journal of Parkinson's disease and Alzheimer's disease, 5(1), 10.13188/2376-922X.1000025.
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2

Immunostaining of Alzheimer's Markers

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Mouse monoclonal or rabbit polyclonal antibodies to tau (ab64193), phospho-Tau (S396) (ab109390), phospho-Tau (T205) (ab4841), ubiquitin (Ubi-1), AβPP (ab15272), Aβ42 (ab10148), SNAP-25 (ab66066), and synaptophysin (ab14692) were purchased from Abeam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was obtained from Proteintech Inc (Chicago, IL). The Amplex UltraRed soluble fluorophore HRP-substrate and 4-MUP alkaline phosphatase substrate were from Invitrogen (Carlsbad, CA). T3D-959 was obtained from T3D Therapeutics, Inc. (Research Triangle Park, NC).
de la Monte S.M., Tong M., Schiano I, & Didsbury J. (2017). Improved Brain Insulin/IGF Signaling and Reduced Neuroinflammation with T3D-959 in an Experimental Model of Sporadic Alzheimer’s Disease. Journal of Alzheimer's disease : JAD, 55(2), 849-864.
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3

Immunohistochemistry Panel for Neurodegenerative Markers

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Antibodies to tau (ab64193), phospho S396-tau (ab109390), phospho T205-tau (ab4841), ubiquitin (Ubi-1), myelin-associated glycoprotein (MAG-1; ab89780), glial fibrillary acidic protein (GFAP; ab7260), and AβPP-Aβ 1→42 (ab10148) were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibody to RPLPO (RPL23 16086-1-AP) was purchased from Proteintech Inc (Chicago, IL). Amplex UltraRed soluble fluorophore HRP-substrate, and 4-MUP alkaline phosphatase substrate were purchased from Invitrogen (Carlsbad, CA). T3D-959 was provided by T3D Therapeutics, Inc. (Research Triangle Park, NC).
Tong M., Deochand C., Didsbury J, & de la Monte S.M. (2016). T3D-959: A Multi-Faceted Disease Remedial Drug Candidate for the Treatment of Alzheimer’s Disease. Journal of Alzheimer's disease : JAD, 51(1), 123-138.
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4

Multiparametric Immunohistochemistry of Neurodegenerative Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to tau (ab64193), S396 (ab 109390) and T205 (ab4841) phospho-tau, ubiquitin (UBI-1 ab7780), myelin-associated glycoprotein (MAG-1; ab89780), glial fibrillary acidic protein (GFAP; ab7260), 3-nitrotyrosine (NTyR; ab61392), Hu neuronal marker (ab78467), Complex IV (abl4744), ATP synthase (abl81243), 8-hydroxyguanosine (8-OHdG; abl0802), common leukocyte antigen (CD45; abl0558), and 4-hydroxynonenal (HNE; ab46545), and the EDH cytotoxicity assay were purchased from Abeam (Cambridge, MA). Rabbit polyclonal antibody to RPEPO (RPE23 16086-1-AP) was purchased from Proteintech Inc (Chicago, IL). Amplex Ultra Red soluble HRP fluorophore and 4-MUP alkaline phosphatase substrate were purchased from Invitrogen (Carlsbad, CA). The L165, 041 PPAR-δ agonist and F-L-Leu PPAR-γ agonist were purchased from Cal Biochem (San Diego, CA).
Reich D., Gallucci G., Tong M, & de la Monte S. (2018). Therapeutic Advantages of Dual Targeting of PPAR-δ and PPAR-γ in an Experimental Model of Sporadic Alzheimer’s Disease. Journal of Parkinson's disease and Alzheimer's disease, 5(1), 10.13188/2376-922X.1000025.
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