Amorphous silica gel (K60) with average particle size of 0.040–0.063 mm was purchased from Sigma-Aldrich. Gold(iii ) chloride 64.4% min was purchased from Alfa Aesar. Analytical grade solvents including acetone and ethanol, as well as 0.1 M HCl used for adsorption studies and ICP-OES sample preparation, were purchased from Fisher Scientific. All chemicals were used as received.
0.1 m hcl
Manufactured by Thermo Fisher Scientific
Sourced in Germany
0.1 M HCl is a hydrochloric acid solution with a concentration of 0.1 moles per liter. It is a common laboratory reagent used for various analytical and experimental purposes.
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Lab products found in correlation
3 protocols using 0.1 m hcl
1
Synthesis of Gold Nanoparticles
2
Quantifying Cell Viability and Proliferation
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24 hours post transfection, 30 × 103 cells per well were re-seeded into 12-well plates. After additional 48 h of incubation, cells were incubated for 1.5 h with MTT-containing medium prepared by dissolving thiazolyl blue (Carl Roth, Karlsruhe, Germany). The cells were then solubilised using 10% Triton X-100 (Carl Roth)/0.1 M HCl (Fisher Scientific, Schwerte, Germany) in isopropanol (Sigma-Aldrich, Taufkirchen, Germany) and the extinction was then measured at 570 nm on a plate reader. For the cell survival assay, 10 × 103 cells were seeded per well in seven 24-well plates and transfected with siRNAs next day. Over a period of 7 days, MTT assays were performed and cell survival was plotted as relative cell number by measuring the MTT extinction coefficient for each day.
3
Whey Protein Isolate Fibril Formation
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Whey protein isolate (Arla Food
Ingredients) was dissolved in 0.1 M HCl (Fisher Scientific) to a concentration
of ca. 200 g/L. The WPI solution was dialyzed against
0.01 M HCl for 24 h at room temperature using a membrane (Spectrum
Laboratories) with a 6–8 kDa molecular weight cutoff to remove
salts and small molecules. The HCl solution was changed three times
during the dialysis. The dialyzed WPI solution (pH 2) was then diluted
to ca. 80 or 40 g/L and incubated at 90 °C for
3 days to form curved and straight PNFs, respectively.6 (link),17 (link) The obtained PNFs were purified by dialysis against 0.01 M HCl for
3 days using a 100 kDa molecular weight cutoff membrane. Furthermore,
the solution of straight fibrils was concentrated by centrifugal membrane
filtration with a 100 kDa cutoff. The concentration of the final PNF
solution before spinning was determined by measuring the dry weight
of the lyophilized materials and was found to be ca. 20 g/L. Different concentrations of genipin (Zhixin Biotechnology,
China), 1, 2, 5, and 10 wt % with respect to the dry mass of PNFs,
were used to prepare the spinning suspensions for the production of
a cross-linked microfiber. The genipin was ground before adding it
to the PNF samples, and the suspension was then vortexed for 1 min
before spinning.
Ingredients) was dissolved in 0.1 M HCl (Fisher Scientific) to a concentration
of ca. 200 g/L. The WPI solution was dialyzed against
0.01 M HCl for 24 h at room temperature using a membrane (Spectrum
Laboratories) with a 6–8 kDa molecular weight cutoff to remove
salts and small molecules. The HCl solution was changed three times
during the dialysis. The dialyzed WPI solution (pH 2) was then diluted
to ca. 80 or 40 g/L and incubated at 90 °C for
3 days to form curved and straight PNFs, respectively.6 (link),17 (link) The obtained PNFs were purified by dialysis against 0.01 M HCl for
3 days using a 100 kDa molecular weight cutoff membrane. Furthermore,
the solution of straight fibrils was concentrated by centrifugal membrane
filtration with a 100 kDa cutoff. The concentration of the final PNF
solution before spinning was determined by measuring the dry weight
of the lyophilized materials and was found to be ca. 20 g/L. Different concentrations of genipin (Zhixin Biotechnology,
China), 1, 2, 5, and 10 wt % with respect to the dry mass of PNFs,
were used to prepare the spinning suspensions for the production of
a cross-linked microfiber. The genipin was ground before adding it
to the PNF samples, and the suspension was then vortexed for 1 min
before spinning.
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