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Ab 217787

Manufactured by Abcam
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Sourced in United Kingdom, United States

Ab 217787 is a primary antibody produced in rabbit and directed against human alpha-Synuclein. It is designed for use in various immunoassays.

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Lab products found in correlation

Bond polymer refine dab detection kit, by Leica (1 mentions) Bond max autostainer, by Leica (1 mentions) Ab52771, by Abcam (1 mentions)

2 protocols using ab 217787

1

Immunohistochemical Analysis of RNF43 and LRP1B

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Immunohistochemical staining was performed on tissue sections of the primary tumor using the Bondmax Autostainer (Leica Biosystems, Germany) and polyclonal antibodies directed against RNF43 (ab 217787, Abcam, Cambridge, UK) and LRP1B (HPA069094, Sigma-Aldrich, St. Louis, MO, USA) in 1:100 dilutions27 ,28 . All tissue sections were pretreated for 20 min with ER1-antigen retrieval solution (Leica Biosystems, Germany). For visualization the Bond Polymer Refine Detection Kit DAB (Leica Biosystems, Germany) was used. Counterstaining was performed with hematoxylin.
Holm B., Barsuhn S., Behrens H.M., Krüger S, & Röcken C. (2023). The tumor biological significance of RNF43 and LRP1B in gastric cancer is complex and context-dependent. Scientific Reports, 13, 3191.
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2

Immunohistochemical Analysis of RNF43 and YAP

Cited 1 time
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IHC experiments were performed as described in our study [17 (link)]. IHC assays were performed using an UltraSensitive Streptavidin Peroxidase Kit (KIT-9710; Maixin Biotechnologies, Beijing, China) according to the manufacturer’s instructions. The following primary antibodies were used: rabbit anti-RNF43 antibody (ab217787; Abcam, Cambridge, MA, USA) and rabbit anti-YAP antibody (ab52771). RNF43 staining was scored using an H-score of 0–3 intensity multiplied by the percentage of positive cells (range 0–300), which was described in our study [17 (link)].
ZHU D., ZHANG L., SHI X., GAO S., YUE C., ZHANG L., BAI Y., WANG Q., OKADA A., YASUI T., WANG C., CUI X, & ZUO L. (2022). RNF43 is a novel tumor-suppressor and prognostic indicator in clear cell renal cell carcinoma. Oncology Research, 29(3), 159-174.
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