(i) the 40% m/v acrylamide (Amersham Biosciences, Uppsala, Sweden) solution was prepared in DEI and stored at 4 °C; (ii) 2% m/v bis-acrylamide (N,N-methylenebisacrylamide, Amersham Biosciences, Uppsala, Sweden) was prepared in DEI and stored at 4 °C; (iii) the phosphate-buffered saline stock solution (stock PBS) was prepared by dissolving 8.0 g NaCl (Lachema, Brno, Czechia), 0.2 g KCl (Lachema, Brno, Czechia), 1.44 g Na2HPO4 (Sigma-Aldrich, Steinheim, Germany), and 0.24 g KH2PO4 (Sigma-Aldrich, Steinheim, Germany) in 100 mL of DEI and the pH of the solutions was adjusted to 7.4 by 1 M HCl; iv) the 10% m/v paraformaldehyde (Lachema, Brno, Czechia) solution was dissolved in hot DEI and stored at 4 °C.
Acrylamide
Manufactured by Cytiva
Sourced in Sweden
Acrylamide is a lab equipment product used for electrophoresis applications. It is a chemical compound commonly used as a gel matrix material in polyacrylamide gel electrophoresis (PAGE) techniques to separate and analyze biological macromolecules such as proteins and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
N n methylenebisacrylamide, by Cytiva (2 mentions)
Milli q water, by Merck Group (2 mentions)
Thiourea, by Cytiva (2 mentions)
Kh2po4, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Pbs solution, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal antibody to human β actin, by Merck Group (1 mentions)
Mitotracker mitochondria red cmxros probe, by Thermo Fisher Scientific (1 mentions)
Protein a g plus agarose sc 2003, by Santa Cruz Biotechnology (1 mentions)
4 protocols using acrylamide
1
Preparation of Electrophoresis Solutions
2
Prohibitin Protein Detection Protocol
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All chemicals were purchased from Sigma Chemical Co. (St Louis, MO, USA) unless otherwise stated. PBS solution was purchased from Ambion Inc. (Austin, TX, USA). All fluorescent probes were purchased from Molecular Probes Inc. (Eugene, OR, USA). Rabbit polyclonal antibody to human Prohibitin was from Abcam Inc. (Cambridge, MA, USA) while mouse monoclonal antibody to human β actin from Sigma Chemical Co. Horseradish peroxidase‐conjugated secondary antibody goat anti‐rabbit/mouse IgG was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). 3‐[(3‐Cholamidopropyl) dimethylammonio]‐1‐propane sulfonate (CHAPS), urea, thiourea, dithiothuretiol (DTT), and acrylamide were purchased from Amersham Biosciences (Uppsala, Sweden). All buffers were prepared with Milli‐Q water (Millipore, Bedford, MA, USA).
3
Mitochondrial Characterization Protocol
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All chemicals were purchased from Sigma Chemical Co. (St. Louis, MO, USA) unless otherwise stated. Phosphate-buffered saline (PBS) solution was purchased from Ambion Inc. (Austin, TX, USA). MitoTracker mitochondria Red CMXRos probe (M7512) was purchased from Molecular Probes Inc. (Eugene, OR, USA). Normal goat serum (NGS), mouse immunoglobulin G (IgG), rabbit IgG, and VECTASHIELD Mounting Medium with 4',6-diamidino-2-phenylindole (DAPI; H1200) were from Vector Laboratories, Inc. (Burlingame, CA, USA). The PI3K inhibitor (wortmannin; #9951) was from Cell Signaling Technology, Inc. (Danvers, MA, USA). Protein A/G PLUS-Agarose (sc-2003) was from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). 3-([3-Cholamidopropyl] dimethylammonio)-1-propane sulfonate (CHAPS), urea, thiourea, dithiothreitol (DTT), and acrylamide were purchased from Amersham Biosciences (Uppsala, Sweden). All buffers were prepared using Milli-Q water (Millipore, Bedford, MA, USA).
4
Casein-Starch Composite Characterization
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Casein (sodium salt, from bovine milk, cat.no. C-8654) and starch (from rice, cat.no. S-7260) were purchased from Sigma-Aldrich, USA. Quick Start 1 × Bradford dye reagent (cat.no. 5000205) was from Bio-Rad, Bio-Rad laboratories, Hercules, California. Acrylamide (cat.no. 17-1302-02) and N, N′-methylenebisAcrylamide (cat.no. 17-1304-02) were purchased from Amersham Biosciences, USA. Sodium dodecylsulfate (cat.no. DB0485), soluble starch (cat.no.SB0904) and β-mercaptoethanol (cat.no. MB0338) were from Bio Basic Canada Inc. 3,5-Dinitrosalicylic acid was purchased from Fluka, USA. Chemical reagents for preparation of buffers were purchased from Carlo Erba (France), Scharlau (Barcelona, Spain), and Ajax Finechem (MA, USA).
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