Apex ft icr mass spectrometer

Manufactured by Bruker

The Apex FT/ICR mass spectrometer is a high-resolution mass spectrometer that utilizes Fourier Transform Ion Cyclotron Resonance (FT-ICR) technology. It is designed to provide precise and accurate mass measurements of a wide range of molecular species.

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Lab products found in correlation

Keap1, by Santa Cruz Biotechnology (1 mentions) Primary antibodies against nrf2, by Santa Cruz Biotechnology (1 mentions) Trxr1, by Santa Cruz Biotechnology (1 mentions) Secondary antibodies conjugated with horseradish peroxidase hrp, by Merck Group (1 mentions) Hydrogen peroxide h2o2, by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Polyethylene glycol 400 peg 400, by Merck Group (1 mentions) Finnigan surveyor hplc system, by Thermo Fisher Scientific (1 mentions) Luna rp c18 column, by Phenomenex (1 mentions) Dd2 spectrometer, by Agilent Technologies (1 mentions) P 2000 digital polarimeter, by Jasco (1 mentions) 515 hplc pump, by Waters Corporation (1 mentions) Evolution 201 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Ultraflextreme maldi tof tof mass spectrometer, by Bruker (1 mentions) Sciex 4000 qtrap mass spectrometer, by AB Sciex (1 mentions)

3 protocols using apex ft icr mass spectrometer

Quantitative Analysis of Bixin

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Analytical grade cis-bixin (9-cis-6,6′-diapo-ψ,ψ-carotenedioic acid, 6-methyl ester) was purchased from Spectrum (CAS number: 6983-79-5). LC/MS confirmation of purity (>98% by weight) was performed using electrospray mass spectrometry of bixin [dissolved in tetrahydrofuran and diluted 10-fold in acetonitrile/NH₄OH (0.1 N); ESI-MS (negative ion mode) m/z 393.21 (M - 1)^-] employing a Bruker Apex FT/ICR mass spectrometer, as specified before (Tao et al., 2015 (link)). Polyethylene glycol 400 (PEG400) was from EMD Millipore. Xanthotoxin (8-methoxypsoralen, 8-MOP), and hydrogen peroxide (H₂O₂) were from Sigma. Primary antibodies against NRF2, KEAP1, TRXR1, GCLM, NQO1, HO1, OGG1, GAPDH, and actin were purchased from Santa Cruz Biotechnology. Primary antibody against p62 was from Abnova. Secondary antibodies conjugated with horseradish peroxidase (HRP) were purchased from Sigma. Antibody against 8-oxo-deoxyguanosine (8-oxo-dG) was from Trevigen.

Rojo de la Vega M., Zhang D.D, & Wondrak G.T. (2018). Topical Bixin Confers NRF2-Dependent Protection Against Photodamage and Hair Graying in Mouse Skin. Frontiers in Pharmacology, 9, 287.

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Bixin Analysis by Mass Spectrometry

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Electrospray mass spectrometry of bixin [dissolved in tetrahydrofuran and diluted tenfold in acetonitrile/NH₄OH (0.1 N); ESI-MS (negative ion mode) m/z 393.21 (M-1)⁻] was performed using a Bruker Apex FT/ICR mass spectrometer. For determination of bixin plasma levels, mouse samples were subjected to chloroform extraction followed by analysis using a Thermo Finnigan Surveyor HPLC system with photodiode array detector (300-580 nm) using a Luna RP-C18 column (3 μ; 100 × 4.6 mm; Phenomenex, Torrance, CA) with mobile phase A (water, 0.1 % formic acid) and mobile phase B (acetonitrile, 0.1 % formic acid); gradient: 0 min: 20% A; 10 min: 5% A; 15 min 0% A.

Tao S., Park S.L., de la Vega M.R., Zhang D.D, & Wondrak G.T. (2015). Systemic administration of the apocarotenoid bixin protects skin against solar UV-induced damage through activation of NRF2. Free radical biology & medicine, 89, 690-700.

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Detailed Analytical Characterization of Compounds

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Optical rotations were acquired on a JASCO P-2000 Digital polarimeter. UV spectra were measured using a Thermo Evolution 201 spectrophotometer. All NMR spectra were acquired in 1:2 pyridine-d5/MeOH-d4 on a 600 MHz Agilent DD2 spectrometer with a 5 mm OneNMR probe. NMR data were analyzed using MestReNova 8.1. High-resolution mass spectra (HRMS) were obtained using a Bruker Apex FT-ICR mass spectrometer with an ESI interface (compound 1) and a Bruker ultrafleXtreme MALDI-TOF/TOF mass spectrometer with HCCA matrix (compound 2). MS/MS experiments were conducted on an AB SCIEX 4000 QTRAP mass spectrometer, with a collision energy of 35 eV. Semi-preparative HPLC was accomplished using a system with two Waters 515 HPLC pumps, a gradient controller, and a Waters 2996 diode array UV detector.

Cai P., He S., Zhou C., Place A.R., Haq S., Ding L., Chen H., Jiang Y., Guo C., Xu Y., Zhang J, & Yan X. (2016). Two new karlotoxins found in Karlodinium veneficum (strain GM2) from the East China Sea. Harmful algae, 58, 66-73.

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